RT Journal Article SR Electronic(1) A1 Mattila, R. K. A1 Harila, K. A1 Kangas, S. M. A1 Paavilainen, H. A1 Heape, A. M. A1 Mohr, I. J. A1 Hukkanen, V.YR 2015 T1 An investigation of herpes simplex virus type 1 latency in a novel mouse dorsal root ganglion model suggests a role for ICP34.5 in reactivation JF Journal of General Virology, VO 96 IS 8 SP 2304 OP 2313 DO https://doi.org/10.1099/vir.0.000138 PB Microbiology Society, SN 1465-2099, AB After a primary lytic infection at the epithelia, herpes simplex virus type 1 (HSV-1) enters the innervating sensory neurons and translocates to the nucleus, where it establishes a quiescent latent infection. Periodically, the virus can reactivate and the progeny viruses spread back to the epithelium. Here, we introduce an embryonic mouse dorsal root ganglion (DRG) culture system, which can be used to study the mechanisms that control the establishment, maintenance and reactivation from latency. Use of acyclovir is not necessary in our model. We examined different phases of the HSV-1 life cycle in DRG neurons, and showed that WT HSV-1 could establish both lytic and latent form of infection in the cells. After reactivating stimulus, the WT viruses showed all markers of true reactivation. In addition, we showed that deletion of the γ134.5 gene rendered the virus incapable of reactivation, even though the virus was clearly able to replicate and persist in a quiescent form in the DRG neurons., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.000138