1887

Abstract

Oropouche virus (OROV) is a public health threat in South America, and in particular in northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches, we determined the complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil, in 2009. We also report the complete genome sequences of two OROV reassortants isolatd from two marmosets in Minas Gerais state, south-east Brazil, in 2012 that contained a novel M genome segment. Interestingly, all 10 isolates possessed a 947 nt S segment that lacked 11 residues in the S-segment 3′ UTR compared with the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated, and the identification of yet another reassortant highlights the importance of bunyavirus surveillance in South America.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.000108
2015-07-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/7/1636.html?itemId=/content/journal/jgv/10.1099/vir.0.000108&mimeType=html&fmt=ahah

References

  1. Acrani G. O., Tilston-Lunel N. L., Spiegel M., Weidemann M., Dilcher M., Andrade da Silva D. E., Nunes M. R. T., Elliott R. M.. ( 2014; ). Establishment of a minigenome system for Oropouche virus reveals the S genome segment to be significantly longer than reported previously. . J Gen Virol 96:, 513–523. [CrossRef] [PubMed]
    [Google Scholar]
  2. Aguilar P. V., Barrett A. D., Saeed M. F., Watts D. M., Russell K., Guevara C., Ampuero J. S., Suarez L., Cespedes M. et al. ( 2011; ). Iquitos virus: a novel reassortant Orthobunyavirus associated with human illness in Peru. . PLoS Negl Trop Dis 5:, e1315. [CrossRef] [PubMed]
    [Google Scholar]
  3. Anderson C. R., Spence L., Downs W. G., Aitken T. H.. ( 1961; ). Oropouche virus: a new human disease agent from Trinidad, West Indies. . Am J Trop Med Hyg 10:, 574–578.[PubMed]
    [Google Scholar]
  4. Azevedo R. S. S., Nunes M. R. T., Chiang J. O., Bensabath G., Vasconcelos H. B., Pinto A. Y. N., Martins L. C., Monteiro H. A. O., Rodrigues S. G., Vasconcelos P. F.. ( 2007; ). Reemergence of Oropouche fever, northern Brazil. . Emerg Infect Dis 13:, 912–915. [CrossRef] [PubMed]
    [Google Scholar]
  5. Baisley K. J., Watts D. M., Munstermann L. E., Wilson M. L.. ( 1998; ). Epidemiology of endemic Oropouche virus transmission in upper Amazonian Peru. . Am J Trop Med Hyg 59:, 710–716.[PubMed]
    [Google Scholar]
  6. Borborema C. A., Pinheiro F. P., Albuquerque B. C., da Rosa A. P., da Rosa J. F., Dourado H. V.. ( 1982; ). [1st occurrence of outbreaks caused by Oropouche virus in the State of Amazonas]. . Rev Inst Med Trop Sao Paulo 24:, 132–139 (in Portuguese).[PubMed]
    [Google Scholar]
  7. Briese T., Calisher C. H., Higgs S.. ( 2013; ). Viruses of the family Bunyaviridae: are all available isolates reassortants?. Virology 446:, 207–216. [CrossRef] [PubMed]
    [Google Scholar]
  8. Buchholz U. J., Finke S., Conzelmann K. K.. ( 1999; ). Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. . J Virol 73:, 251–259.[PubMed]
    [Google Scholar]
  9. Darriba D., Taboada G. L., Doallo R., Posada D.. ( 2012; ). jModelTest 2: more models, new heuristics and parallel computing. . Nat Methods 9:, 772. [CrossRef] [PubMed]
    [Google Scholar]
  10. Dixon K. E., Travassos da Rosa A. P., Travassos da Rosa J. F., Llewellyn C. H.. ( 1981; ). Oropouche virus. II. Epidemiological observations during an epidemic in Santarém, Pará, Brazil in 1975. . Am J Trop Med Hyg 30:, 161–164.[PubMed]
    [Google Scholar]
  11. Dunn E. F., Pritlove D. C., Elliott R. M.. ( 1994; ). The S RNA genome segments of Batai, Cache Valley, Guaroa, Kairi, Lumbo, Main Drain and Northway bunyaviruses: sequence determination and analysis. . J Gen Virol 75:, 597–608. [CrossRef] [PubMed]
    [Google Scholar]
  12. Elliott R. M.. ( 2014; ). Orthobunyaviruses: recent genetic and structural insights. . Nat Rev Microbiol 12:, 673–685. [CrossRef] [PubMed]
    [Google Scholar]
  13. Elliott R. M., Blakqori G.. ( 2011; ). Molecular biology of orthobunyaviruses. . In Bunyaviridae: Molecular and Cellular Biology, pp. 1–39. Edited by Plyusnin A., Elliott R. M... Norfork, UK:: Caister Academic Press;.
    [Google Scholar]
  14. Figueiredo L. T. M., Da Rosa A. P. A. T.. ( 1988; ). Jatobal virus antigenic characterization by ELISA and neutralization test using EIA as indicator, on tissue culture. . Mem Inst Oswaldo Cruz 83:, 161–164. [CrossRef] [PubMed]
    [Google Scholar]
  15. Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O.. ( 2010; ). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. . Syst Biol 59:, 307–321. [CrossRef] [PubMed]
    [Google Scholar]
  16. Ladner J. T., Savji N., Lofts L., Travassos da Rosa A., Wiley M. R., Gestole M. C., Rosen G. E., Guzman H., Vasconcelos P. F. et al. ( 2014; ). Genomic and phylogenetic characterization of viruses included in the Manzanilla and Oropouche species complexes of the genus Orthobunyavirus, family Bunyaviridae. . J Gen Virol 95:, 1055–1066. [CrossRef] [PubMed]
    [Google Scholar]
  17. LeDuc J. W., Hoch A. L., Pinheiro F. P., da Rosa A. P.. ( 1981; ). Epidemic Oropouche virus disease in northern Brazil. . Bull Pan Am Health Organ 15:, 97–103.[PubMed]
    [Google Scholar]
  18. Lole K. S., Bollinger R. C., Paranjape R. S., Gadkari D., Kulkarni S. S., Novak N. G., Ingersoll R., Sheppard H. W., Ray S. C.. ( 1999; ). Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. . J Virol 73:, 152–160.[PubMed]
    [Google Scholar]
  19. Lowen A. C., Elliott R. M.. ( 2005; ). Mutational analyses of the nonconserved sequences in the Bunyamwera Orthobunyavirus S segment untranslated regions. . J Virol 79:, 12861–12870. [CrossRef] [PubMed]
    [Google Scholar]
  20. Margulies M., Egholm M., Altman W. E., Attiya S., Bader J. S., Bemben L. A., Berka J., Braverman M. S., Chen Y.-J. et al. ( 2005; ). Genome sequencing in microfabricated high-density picolitre reactors. . Nature 437:, 376–380.[PubMed]
    [Google Scholar]
  21. Martin D. P., Lemey P., Lott M., Moulton V., Posada D., Lefeuvre P.. ( 2010; ). rdp3: a flexible and fast computer program for analyzing recombination. . Bioinformatics 26:, 2462–2463. [CrossRef] [PubMed]
    [Google Scholar]
  22. Mazel-Sanchez B., Elliott R. M.. ( 2012; ). Attenuation of bunyamwera orthobunyavirus replication by targeted mutagenesis of genomic untranslated regions and creation of viable viruses with minimal genome segments. . J Virol 86:, 13672–13678. [CrossRef] [PubMed]
    [Google Scholar]
  23. Nunes M. R. T., Martins L. C., Rodrigues S. G., Chiang J. O., Azevedo R. S. S., Travassos da Rosa A. P. A., Vasconcelos P. F. C.. ( 2005; a). Oropouche virus isolation, southeast Brazil. . Emerg Infect Dis 11:, 1610–1613. [CrossRef] [PubMed]
    [Google Scholar]
  24. Nunes M. R. T., Travassos da Rosa A. P. A., Weaver S. C., Tesh R. B., Vasconcelos P. F. C.. ( 2005; b). Molecular epidemiology of group C viruses (Bunyaviridae, Orthobunyavirus) isolated in the Americas. . J Virol 79:, 10561–10570. [CrossRef] [PubMed]
    [Google Scholar]
  25. Pinheiro F. P., Pinheiro M., Bensabath G., Causey O. R., Shope R. E.. ( 1962; ). Epidemia de vírus Oropouche em Belém. . Rev Serv Especial Saude 12:, 15–23.
    [Google Scholar]
  26. Pinheiro F. P., Travassos da Rosa A. P., Travassos da Rosa J. F., Bensabath G.. ( 1976; ). An outbreak of Oropouche virus diease in the vicinity of Santarem, Para, Brazil. . Tropenmed Parasitol 27:, 213–223.[PubMed]
    [Google Scholar]
  27. Pinheiro F. P., Hoch A. L., Gomes M. L., Roberts D. R.. ( 1981; ). Oropouche virus. IV. Laboratory transmission by Culicoides paraensis. . Am J Trop Med Hyg 30:, 172–176.[PubMed]
    [Google Scholar]
  28. Pinheiro F. P., Travassos da Rosa A. P., Gomes M. L., LeDuc J. W., Hoch A. L.. ( 1982; ). Transmission of Oropouche virus from man to hamster by the midge Culicoides paraensis. . Science 215:, 1251–1253. [CrossRef] [PubMed]
    [Google Scholar]
  29. Roberts D. R., Pinheiro F. P., Hoch A. L., LeDuc J. W., Peterson N. E., Santos M. A. V., Western K. A..Pan American Health Organization Washington DC. ( 1977; ). Vectors and natural reservoirs of Oropouche virus in the Amazon region. Tech. Rep. NTIS 7815, US Dep. Commer Washington, DC:: access. no. AD-A053;.
    [Google Scholar]
  30. Ronquist F., Huelsenbeck J. P.. ( 2003; ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19:, 1572–1574. [CrossRef] [PubMed]
    [Google Scholar]
  31. Rothberg J. M., Hinz W., Rearick T. M., Schultz J., Mileski W., Davey M., Leamon J. H., Johnson K., Milgrew M. J. et al. ( 2011; ). An integrated semiconductor device enabling non-optical genome sequencing. . Nature 475:, 348–352. [CrossRef] [PubMed]
    [Google Scholar]
  32. Saeed M. F., Wang H., Nunes M., Vasconcelos P. F., Weaver S. C., Shope R. E., Watts D. M., Tesh R. B., Barrett A. D.. ( 2000; ). Nucleotide sequences and phylogeny of the nucleocapsid gene of Oropouche virus. . J Gen Virol 81:, 743–748.[PubMed]
    [Google Scholar]
  33. Saeed M. F., Wang H., Suderman M., Beasley D. W., Travassos da Rosa A., Li L., Shope R. E., Tesh R. B., Barrett A. D.. ( 2001; ). Jatobal virus is a reassortant containing the small RNA of Oropouche virus. . Virus Res 77:, 25–30. [CrossRef] [PubMed]
    [Google Scholar]
  34. Sievers F., Wilm A., Dineen D., Gibson T. J., Karplus K., Li W., Lopez R., McWilliam H., Remmert M. et al. ( 2011; ). Fast, scalable generation of high-quality protein multiple sequence alignments using . Clustal Omega. . Mol Syst Biol 7:, 539. [CrossRef] [PubMed]
    [Google Scholar]
  35. Suyama M., Torrents D., Bork P.. ( 2006; ). pal2nal: robust conversion of protein sequence alignments into the corresponding codon alignments. . Nucleic Acids Res 34: (Web Server issue), W609–W612. [CrossRef] [PubMed]
    [Google Scholar]
  36. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013; ). mega6: Molecular Evolutionary Genetics Analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef] [PubMed]
    [Google Scholar]
  37. Vasconcelos P. F. C., Travassos Da Rosa J. F. S., Guerreiro S. C., Dégallier N., Travassos Da Rosa E. S., Travassos Da Rosa A. P. A.. ( 1989; ). [1st register of an epidemic caused by Oropouche virus in the states of Maranhão and Goiás, Brazil]. . Rev Inst Med Trop Sao Paulo 31:, 271–278 (in Portuguese). [CrossRef] [PubMed]
    [Google Scholar]
  38. Vasconcelos H. B., Azevedo R. S. S., Casseb S. M., Nunes-Neto J. P., Chiang J. O., Cantuária P. C., Segura M. N. O., Martins L. C., Monteiro H. A. O. et al. ( 2009; ). Oropouche fever epidemic in Northern Brazil: epidemiology and molecular characterization of isolates. . J Clin Virol 44:, 129–133. [CrossRef] [PubMed]
    [Google Scholar]
  39. Vasconcelos H. B., Nunes M. R., Casseb L. M., Carvalho V. L., Pinto da Silva E. V., Silva M., Casseb S. M., Vasconcelos P. F.. ( 2011; ). Molecular epidemiology of Oropouche virus, Brazil. . Emerg Infect Dis 17:, 800–806. [CrossRef] [PubMed]
    [Google Scholar]
  40. Weber F., Dunn E. F., Bridgen A., Elliott R. M.. ( 2001; ). The Bunyamwera virus nonstructural protein NSs inhibits viral RNA synthesis in a minireplicon system. . Virology 281:, 67–74. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.000108
Loading
/content/journal/jgv/10.1099/vir.0.000108
Loading

Data & Media loading...

Supplements

Supplementary Data



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error