RT Journal Article SR Electronic(1) A1 Wilson, Garrick K. A1 Farquhar, Michelle J. A1 Meredith, Luke A1 Dhawan, Anil A1 Mitry, Ragai A1 Balfe, Peter A1 McKeating, Jane A.YR 2015 T1 Permissivity of primary hepatocytes and hepatoma cell lines to support hepatitis C virus infection JF Journal of General Virology, VO 96 IS 6 SP 1369 OP 1373 DO https://doi.org/10.1099/vir.0.000085 PB Microbiology Society, SN 1465-2099, AB The major cell type supporting hepatitis C virus (HCV) infection is the hepatocyte; however, most reports studying viral entry and replication utilize transformed hepatoma cell lines. We demonstrate that HCV pseudoparticles (HCVpp) infect primary hepatocytes with comparable rates to hepatoma cells, demonstrating the limited variability in donor hepatocytes to support HCV receptor-glycoprotein-dependent entry. In contrast, we observed a 2-log range in viral replication between the same donor hepatocytes. We noted that cell proliferation augments pseudoparticle reporter activity and arresting hepatoma cells yields comparable levels of infection to hepatocytes. This study demonstrates comparable rates of HCVpp entry into primary hepatocytes and hepatoma cells, validating the use of transformed cells as a model system to study HCV entry., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.000085