1887

Abstract

Thermostable Newcastle disease virus (NDV) vaccines have been used widely to control Newcastle disease for village poultry flocks, due to their independence of cold chains for delivery and storage. To explore the potential use of thermostable NDV as a vaccine vector, an infectious clone of thermostable avirulent NDV strain TS09-C was developed using reverse genetics technology. The GFP gene, along with the self-cleaving 2A gene of foot-and-mouth disease virus and ubiquitin monomer (2AUbi), were inserted immediately upstream of the NP (nucleocapsid protein), M (matrix protein) or L (large polymerase protein) gene translation start codon in the TS09-C infectious clone. Detection of GFP expression in the recombinant virus-infected cells showed that the recombinant virus, rTS-GFP/M, with the GFP gene inserted into the M gene expressed the highest level of GFP. The rTS-GFP/M virus retained the same thermostability, growth dynamics and pathogenicity as its parental rTS09-C virus. Vaccination of specific-pathogen-free chickens with the rTS-GFP/M virus conferred complete protection against virulent NDV challenge. Taken together, the data suggested that the rTS09-C virus could be used as a vaccine vector to develop bivalent thermostable vaccines against Newcastle disease and the target avian diseases for village chickens, especially in the developing and least-developed countries.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.000067
2015-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/6/1219.html?itemId=/content/journal/jgv/10.1099/vir.0.000067&mimeType=html&fmt=ahah

References

  1. Aini I., Ibrahim A. L., Spradbrow P. B. (1990).; Field trials of a food-based vaccine to protect village chickens against Newcastle disease. Res Vet Sci 49:216–219[PubMed]
    [Google Scholar]
  2. Alexander D. J. (1998).; Newcastle disease virus and other avian paramyxoviruses. In A Laboratory Manual for the Isolation and Identification of Avian Pathogens pp. 156–163 Edited by Swayne D., Glisson J. R., Jackwood M. W., Pearson J. E., Reed W. M. Kennett Square, PA: American Association of Avian Pathologists;
    [Google Scholar]
  3. Bancroft B. J., Spradbrow P. B. (1978).; The spread of the V4 strain of Newcastle disease virus between chickens vaccinated by drinking water administration. Aust Vet J 54:500–501 [View Article][PubMed]
    [Google Scholar]
  4. Bell J. G., Fotzo T. M., Amara A., Agbede G. (1995).; A field trial of the heat resistant V4 vaccine against Newcastle disease by eye-drop inoculation in village poultry in Cameroon. Prev Vet Med 25:19–25 [View Article]
    [Google Scholar]
  5. Bensink Z., Spradbrow P. (1999).; Newcastle disease virus strain I2 – a prospective thermostable vaccine for use in developing countries. Vet Microbiol 68:131–139 [View Article][PubMed]
    [Google Scholar]
  6. Carnero E., Li W., Borderia A. V., Moltedo B., Moran T., García-Sastre A. (2009).; Optimization of human immunodeficiency virus Gag expression by Newcastle disease virus vectors for the induction of potent immune responses. J Virol 83:584–597 [View Article][PubMed]
    [Google Scholar]
  7. Das P. (2004).; Revolutionary vaccine technology breaks the cold chain. Lancet Infect Dis 4:719 [View Article][PubMed]
    [Google Scholar]
  8. de Leeuw O., Peeters B. (1999).; Complete nucleotide sequence of Newcastle disease virus: evidence for the existence of a new genus within the subfamily Paramyxovirinae . J Gen Virol 80:131–136[PubMed] [CrossRef]
    [Google Scholar]
  9. DiNapoli J. M., Nayak B., Yang L., Finneyfrock B. W., Cook A., Andersen H., Torres-Velez F., Murphy B. R., Samal S. K. et al. (2010).; Newcastle disease virus-vectored vaccines expressing the hemagglutinin or neuraminidase protein of H5N1 highly pathogenic avian influenza virus protect against virus challenge in monkeys. J Virol 84:1489–1503 [View Article][PubMed]
    [Google Scholar]
  10. Echeonwu G. O. N., Iroegbu G. U., Ngene A., Junaid S. A., Ndako J., Echeonwu I. E., Okoye J. O. (2008a).; Survival of Newcastle disease virus (NDV) strain V4-UPM coated on three grains offal and exposed to room temperature. Afr J Biotechnol 15:2688–2692
    [Google Scholar]
  11. Engel-Herbert I., Werner O., Teifke J. P., Mebatsion T., Mettenleiter T. C., Römer-Oberdörfer A. (2003).; Characterization of a recombinant Newcastle disease virus expressing the green fluorescent protein. J Virol Methods 108:19–28 [View Article][PubMed]
    [Google Scholar]
  12. Garten W., Berk W., Nagai Y., Rott R., Klenk H. D. (1980).; Mutational changes of the protease susceptibility of glycoprotein F of Newcastle disease virus: effects on pathogenicity. J Gen Virol 50:135–147 [View Article][PubMed]
    [Google Scholar]
  13. Ge J., Wang X., Tao L., Wen Z., Feng N., Yang S., Xia X., Yang C., Chen H., Bu Z. (2011).; Newcastle disease virus-vectored rabies vaccine is safe, highly immunogenic, and provides long-lasting protection in dogs and cats. J Virol 85:8241–8252 [View Article][PubMed]
    [Google Scholar]
  14. Hosaka M., Nagahama M., Kim W. S., Watanabe T., Hatsuzawa K., Ikemizu J., Murakami K., Nakayama K. (1991).; Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway. J Biol Chem 266:12127–12130[PubMed]
    [Google Scholar]
  15. Hu H., Roth J. P., Estevez C. N., Zsak L., Liu B., Yu Q. (2011).; Generation and evaluation of a recombinant Newcastle disease virus expressing the glycoprotein (G) of avian metapneumovirus subgroup C as a bivalent vaccine in turkeys. Vaccine 29:8624–8633 [View Article][PubMed]
    [Google Scholar]
  16. Huang Z., Elankumaran S., Yunus A. S., Samal S. K. (2004).; A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Virol 78:10054–10063 [View Article][PubMed]
    [Google Scholar]
  17. Ideris A., Ibrahim A. L., Spradbrow P. B. (1990).; Vaccination of chickens against Newcastle disease with a food pellet vaccine. Avian Pathol 19:371–384 [View Article][PubMed]
    [Google Scholar]
  18. Jayawardane G. W., Spradbrow P. B. (1995).; Cell-mediated immunity in chickens vaccinated with the V4 strain of Newcastle disease virus. Vet Microbiol 46:37–41 [View Article][PubMed]
    [Google Scholar]
  19. Jayawardane G. W., Spradbrow P. B. (1995).; Mucosal immunity in chickens vaccinated with the V4 strain of Newcastle disease virus. Vet Microbiol 46:69–77 [View Article][PubMed]
    [Google Scholar]
  20. Kim S. H., Samal S. K. (2010).; Role of untranslated regions in regulation of gene expression, replication, and pathogenicity of Newcastle disease virus expressing green fluorescent protein. J Virol 84:2629–2634 [View Article][PubMed]
    [Google Scholar]
  21. King D. J. (1993).; Newcastle disease virus passage in MDBK cells as an aid in detection of a virulent subpopulation. Avian Dis 37:961–969 [View Article][PubMed]
    [Google Scholar]
  22. Kolakofsky D., Roux L., Garcin D., Ruigrok R. W. (2005).; Paramyxovirus mRNA editing, the ‘rule of six’ and error catastrophe: a hypothesis. J Gen Virol 86:1869–1877 [View Article][PubMed]
    [Google Scholar]
  23. Lamb R. A., Collins P. L., Kolakofsky D., Melero J. A., Nagai Y., Oldstone M. B. A., Pringle C. R., Rima B. K. (2005).; Family Paramyxoviridae . In Virus Taxonomy: Eighth Report of the International Committee on Taxonomy of Viruses pp. 655–668 Edited by Fauquet C. M., Mayo M. A., Maniloff J., Desselberger U., Ball L. A. London: Academic Press;
    [Google Scholar]
  24. Lomniczi B. (1975).; Thermostability of Newcastle disease virus strains of different virulence. Arch Virol 47:249–255 [View Article][PubMed]
    [Google Scholar]
  25. Mazija H., Cajavec S., Ergotić N., Ciglar-Grozdanić I., Gottstein Z., Ragland W. L. (2010).; Immunogenicity and safety of Queensland V4 and Ulster 2C strains of Newcastle disease virus given to maternally immune, newly hatched chickens by nebulization. Avian Dis 54:99–103 [View Article][PubMed]
    [Google Scholar]
  26. Miller P. J., Guus K. (2013).; Newcastle disease. In Diseases of Poultry, 13th edn. pp. 98–107 Edited by Swayne D. E., Glisson J. R., McDougald L. R., Nolan L. K., Suarez D. L., Nair V. Ames, IA: Wiley-Blackwell;
    [Google Scholar]
  27. Nagai Y., Klenk H. D., Rott R. (1976).; Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72:494–508 [View Article][PubMed]
    [Google Scholar]
  28. Nakaya T., Cros J., Park M. S., Nakaya Y., Zheng H., Sagrera A., Villar E., García-Sastre A., Palese P. (2001).; Recombinant Newcastle disease virus as a vaccine vector. J Virol 75:11868–11873 [View Article][PubMed]
    [Google Scholar]
  29. Pedersen J. C., Senne D. A., Woolcock P. R., Kinde H., King D. J., Wise M. G., Panigrahy B., Seal B. S. (2004).; Phylogenetic relationships among virulent Newcastle disease virus isolates from the 2002–2003 outbreak in California and other recent outbreaks in North America. J Clin Microbiol 42:2329–2334 [View Article][PubMed]
    [Google Scholar]
  30. Peeters B. P., Gruijthuijsen Y. K., de Leeuw O. S., Gielkens A. L. (2000).; Genome replication of Newcastle disease virus: involvement of the rule-of-six. Arch Virol 145:1829–1845 [View Article][PubMed]
    [Google Scholar]
  31. Pritzer E., Kuroda K., Garten W., Nagai Y., Klenk H. D. (1990).; A host range mutant of Newcastle disease virus with an altered cleavage site for proteolytic activation of the F protein. Virus Res 15:237–242 [View Article][PubMed]
    [Google Scholar]
  32. Reed L. J., Muench H. (1938).; A simple method for estimating fifty percent endpoints. Am J Hyg 27:493–497
    [Google Scholar]
  33. Simmons G. C. (1967).; The isolation of Newcastle disease virus in Queensland. Aust Vet J 43:29–30 [View Article][PubMed]
    [Google Scholar]
  34. Spradbrow P. B. (1993/94).; Newcastle disease in village chickens. Poult Sci Rev 5:57–96
    [Google Scholar]
  35. Steward M., Vipond I. B., Millar N. S., Emmerson P. T. (1993).; RNA editing in Newcastle disease virus. J Gen Virol 74:2539–2547 [View Article][PubMed]
    [Google Scholar]
  36. Toro H., Zhao W., Breedlove C., Zhang Z., van Santen V., Yu Q. (2014).; Infectious bronchitis virus S2 expressed from recombinant virus confers broad protection against challenge. Avian Dis 58:83–89 [View Article][PubMed]
    [Google Scholar]
  37. Tscherne D. M., Jones C. T., Evans M. J., Lindenbach B. D., McKeating J. A., Rice C. M. (2006).; Time- and temperature-dependent activation of hepatitis C virus for low-pH-triggered entry. J Virol 80:1734–1741 [View Article][PubMed]
    [Google Scholar]
  38. Tu T. D., Phuc K. V., Dinh N. T. K., Quoc D. N., Spradbrow P. B. (1998).; Vietnamese trials with a thermostable Newcastle disease vaccine (strain I2) in experimental and village chickens. Prev Vet Med 34:205–214 [View Article][PubMed]
    [Google Scholar]
  39. Wen G., Shang Y., Guo J., Chen C., Shao H., Luo Q., Yang J., Wang H., Cheng G. (2013).; Complete genome sequence and molecular characterization of thermostable Newcastle disease virus strain TS09-C. Virus Genes 46:542–545 [View Article][PubMed]
    [Google Scholar]
  40. Wu C., Zhan A., Liu H., Zhang R. (1999).; Studies on effects of trypsin on Newcastle disease virus LaSota strain. J Yangzhou Univ 2:33–36
    [Google Scholar]
  41. Zhao H., Peeters B. P. (2003).; Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication. J Gen Virol 84:781–788 [View Article][PubMed]
    [Google Scholar]
  42. Zhao W., Spatz S., Zhang Z., Wen G., Garcia M., Zsak L., Yu Q. (2014).; Newcastle disease virus (NDV) recombinants expressing infectious laryngotracheitis virus (ILTV) glycoproteins gB and gD protect chickens against ILTV and NDV challenges. J Virol 88:8397–8406 [View Article][PubMed]
    [Google Scholar]
  43. Zhao W., Zhang Z., Zsak L., Yu Q. (2015).; P and M gene junction is the optimal insertion site in Newcastle disease virus vaccine vector for foreign gene expression. J Gen Virol 96:40–45 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.000067
Loading
/content/journal/jgv/10.1099/vir.0.000067
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error