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Abstract

Influenza A viruses circulate in a wide range of animals. H3N8 equine influenza virus (EIV) is an avian-origin virus that has established in dogs as canine influenza virus (CIV) and has also been isolated from camels and pigs. Previous work suggests that mutations acquired during EIV evolution might have played a role in CIV emergence. Given the potential role of pigs as a source of human infections, we determined the ability of H3N8 EIVs to replicate in pig cell lines and in respiratory explants. We show that phylogenetically distinct EIVs display different infection phenotypes along the pig respiratory tract, but not in cell lines. Our results suggest that EIV displays a dynamic host range along its evolutionary history, supporting the view that evolutionary processes play important roles in host range and tropism and also underscoring the utility of using explant cultures to study influenza pathogenesis.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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/content/journal/jgv/10.1099/vir.0.000049
2015-05-01
2022-01-16
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References

  1. Chan R. W. Y., Karamanska R., Van Poucke S., Van Reeth K., Chan I. W. W., Chan M. C. W., Dell A., Peiris J. S. M., Haslam S. M. et al. 2013; Infection of swine ex vivo tissues with avian viruses including H7N9 and correlation with glycomic analysis. Influenza Other Respi Viruses 7:1269–1282 [View Article][PubMed]
    [Google Scholar]
  2. Crawford P. C., Dubovi E. J., Castleman W. L., Stephenson I., Gibbs E. P. J., Chen L., Smith C., Hill R. C., Ferro P. et al. 2005; Transmission of equine influenza virus to dogs. Science 310:482–485 [View Article][PubMed]
    [Google Scholar]
  3. Ferrari M., Scalvini A., Losio M. N., Corradi A., Soncini M., Bignotti E., Milanesi E., Ajmone-Marsan P., Barlati S. et al. 2003; Establishment and characterization of two new pig cell lines for use in virological diagnostic laboratories. J Virol Methods 107:205–212 [View Article][PubMed]
    [Google Scholar]
  4. Gonzalez G., Marshall J. F., Morrell J., Robb D., McCauley J. W., Perez D. R., Parrish C. R., Murcia P. R. 2014; Infection and pathogenesis of canine, equine, and human influenza viruses in canine tracheas. J Virol 88:9208–9219 [View Article][PubMed]
    [Google Scholar]
  5. Guo Y., Wang M., Kawaoka Y., Gorman O., Ito T., Saito T., Webster R. G. 1992; Characterization of a new avian-like influenza A virus from horses in China. Virology 188:245–255 [View Article][PubMed]
    [Google Scholar]
  6. Guo Y., Wang M., Zheng G. S., Li W. K., Kawaoka Y., Webster R. G. 1995; Seroepidemiological and molecular evidence for the presence of two H3N8 equine influenza viruses in China in 1993-94. J Gen Virol 76:2009–2014 [View Article][PubMed]
    [Google Scholar]
  7. Hillaire M. L. B., Haagsman H. P., Osterhaus A. D. M. E., Rimmelzwaan G. F., van Eijk M. 2013; Pulmonary surfactant protein D in first-line innate defence against influenza A virus infections. J Innate Immun 5:197–208 [View Article][PubMed]
    [Google Scholar]
  8. Karlsson E. A., Ip H. S., Hall J. S., Yoon S. W., Johnson J., Beck M. A., Webby R. J., Schultz-Cherry S. 2014; Respiratory transmission of an avian H3N8 influenza virus isolated from a harbour seal. Nat Commun 5:4791 [View Article][PubMed]
    [Google Scholar]
  9. Löndt B. Z., Brookes S. M., Nash B. J., Núñez A., Stagg D. A., Brown I. H. 2012; The infectivity of pandemic 2009 H1N1 and avian influenza viruses for pigs: an assessment by ex vivo respiratory tract organ culture. Influenza Other Respi Viruses 7:339–402[PubMed]
    [Google Scholar]
  10. Ma W., Kahn R. E., Richt J. A. 2008; The pig as a mixing vessel for influenza viruses: Human and veterinary implications. J Mol Genet Med 3:158–166[PubMed]
    [Google Scholar]
  11. Matrosovich M., Klenk H.-D. 2003; Natural and synthetic sialic acid-containing inhibitors of influenza virus receptor binding. Rev Med Virol 13:85–97 [View Article][PubMed]
    [Google Scholar]
  12. Murcia P. R., Wood J. L. N., Holmes E. C. 2011; Genome-scale evolution and phylodynamics of equine H3N8 influenza A virus. J Virol 85:5312–5322 [View Article][PubMed]
    [Google Scholar]
  13. Nunes S. F., Murcia P. R., Tiley L. S., Brown I. H., Tucker A. W., Maskell D. J., Wood J. L. N. 2010; An ex vivo swine tracheal organ culture for the study of influenza infection. Influenza Other Respir Viruses 4:7–15 [View Article][PubMed]
    [Google Scholar]
  14. Smith G. J., Vijaykrishna D., Bahl J., Lycett S. J., Worobey M., Pybus O. G., Ma S. K., Cheung C. L., Raghwani J. et al. 2009; Origins and evolutionary genomics of the 2009 swine-origin H1N1 influenza A epidemic. Nature 459:1122–1125 [View Article][PubMed]
    [Google Scholar]
  15. Tu J., Zhou H., Jiang T., Li C., Zhang A., Guo X., Zou W., Chen H., Jin M. 2009; Isolation and molecular characterization of equine H3N8 influenza viruses from pigs in China. Arch Virol 154:887–890 [View Article][PubMed]
    [Google Scholar]
  16. Van Poucke S. G. M., Nicholls J. M., Nauwynck H. J., Van Reeth K. 2010; Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution. Virol J 7:38 [View Article][PubMed]
    [Google Scholar]
  17. Vincent A., Awada L., Brown I., Chen H., Claes F., Dauphin G., Donis R., Culhane M., Hamilton K. et al. 2014; Review of influenza A virus in swine worldwide: a call for increased surveillance and research. Zoonoses Public Health 61:4–17 [View Article][PubMed]
    [Google Scholar]
  18. Worobey M., Han G.-Z., Rambaut A. 2014; A synchronized global sweep of the internal genes of modern avian influenza virus. Nature 508:254–257 [View Article][PubMed]
    [Google Scholar]
  19. Yondon M., Zayat B., Nelson M. I., Heil G. L., Anderson B. D., Lin X., Halpin R. A., McKenzie P. P., White S. K. et al. 2014; Equine influenza A(H3N8) virus isolated from Bactrian camel, Mongolia. Emerg Infect Dis 20:2144–2147 [View Article][PubMed]
    [Google Scholar]
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