1887

Abstract

Members of the genus , which belongs to the family , can cause great economic loss in fish culture. In the present report, a vesiculovirus [named snakehead fish vesiculovirus (SHVV)] was isolated from diseased hybrid snakehead fish. SHVV shared 94 % nucleotide sequence identity at the genomic level with rhabdovirus (SCRV), which infects mandarin fish (). We showed that SHVV was able to replicate and proliferate well in SSN-1 cells, which originate from striped snakehead fish (). Furthermore, mandarin fish was susceptible to SHVV by bath exposure, as well as by intraperitoneal injection. The infected fish showed typical clinical signs of rhabdovirus infection, including haemorrhage and oedema. Histopathological analysis revealed that extensive inflammation and necrosis were observed in the spleen, kidney, liver, heart and brain of the moribund mandarin fish. These results will shed new light on the epidemic of vesiculovirus infections among fish.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.000037
2015-04-01
2020-09-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/4/775.html?itemId=/content/journal/jgv/10.1099/vir.0.000037&mimeType=html&fmt=ahah

References

  1. Ahne W., Jorgensen P. E. V., Olesen N. J., Wattanavijarn W. 1988; Serological examination of a rhabdovirus isolated from snakehead fish (Ophicephalus striatus) in Thailand with ulcerative syndrome. J Appl Ichthyol 4:194–196 [CrossRef]
    [Google Scholar]
  2. Boudinot P., Bernard D., Boubekeur S., Thoulouze M. I., Bremont M., Benmansour A. 2004; The glycoprotein of a fish rhabdovirus profiles the virus-specific T-cell repertoire in rainbow trout. J Gen Virol 85:3099–3108 [CrossRef][PubMed]
    [Google Scholar]
  3. Frerichs G. N., Rodger H. D., Peric Z. 1996; Cell culture isolation of piscine neuropathy nodavirus from juvenile sea bass, Dicentrarchus labrax . J Gen Virol 77:2067–2071 [CrossRef][PubMed]
    [Google Scholar]
  4. Iverson L. E., Rose J. K. 1981; Localized attenuation and discontinuous synthesis during vesicular stomatitis virus transcription. Cell 23:477–484 [CrossRef][PubMed]
    [Google Scholar]
  5. King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J. editors 2012 Virus Taxonomy: Classification and Nomenclature of Viruses. Ninth Report of the International Committee on Taxonomy of Viruses San Diego: Elsevier Academic Press;
    [Google Scholar]
  6. Kuzmin I. V., Novella I. S., Dietzgen R. G., Padhi A., Rupprecht C. E. 2009; The rhabdoviruses: biodiversity, phylogenetics, and evolution. Infect Genet Evol 9:541–553 [CrossRef][PubMed]
    [Google Scholar]
  7. LaPatra S. E., Evilia C., Winston V. 2008; Positively selected sites on the surface glycoprotein (G) of infectious hematopoietic necrosis virus. J Gen Virol 89:703–708 [CrossRef][PubMed]
    [Google Scholar]
  8. Lyles S. D., Rupprecht E. C. 2006; Rhabdoviridae . In Fields Virology, 5th edn. pp. 1364–1386 Edited by Knipe M. D., Howley M. P. Philadelphia: Lippincott Williams & Wilkins;
    [Google Scholar]
  9. Ou T., Zhu R. L., Chen Z. Y., Zhang Q. Y. 2013; Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus . Dis Aquat Organ 106:197–206 [CrossRef][PubMed]
    [Google Scholar]
  10. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497
    [Google Scholar]
  11. Tao J. J., Zhou G. Z., Gui J. F., Zhang Q. Y. 2008; Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF. Virus Res 132:86–96 [CrossRef][PubMed]
    [Google Scholar]
  12. Villarreal L. P., Breindl M., Holland J. J. 1976; Determination of molar ratios of vesicular stomatitis virus induced RNA species in BHK21 cells. Biochemistry 15:1663–1667 [CrossRef][PubMed]
    [Google Scholar]
  13. Wertz G. W., Perepelitsa V. P., Ball L. A. 1998; Gene rearrangement attenuates expression and lethality of a nonsegmented negative strand RNA virus. Proc Natl Acad Sci U S A 95:3501–3506 [CrossRef][PubMed]
    [Google Scholar]
  14. Zeng W. W., Wang Q., Wang Y. Y. 2013; Isolation and characterization of a rhabdovirus from snakehead fish (Ophicephalus striatus). J Fish China 37:1416–1424 [CrossRef]
    [Google Scholar]
  15. Zeng W. W., Wang Q., Wang Y. Y., Liu C., Liang H. R., Fang X., Wu S. Q. 2014; Genomic characterization and taxonomic position of a rhabdovirus from a hybrid snakehead. Arch Virol 159:2469–2473 [CrossRef][PubMed]
    [Google Scholar]
  16. Zhang Q. Y., Li Z. Q., Gui J. F. 2000; Isolation of a lethal rhabdovirus from the cultured Chinese sucker Myxocyprinus asiaticus . Dis Aquat Organ 42:1–9 [CrossRef][PubMed]
    [Google Scholar]
  17. Zhang Q. Y., Tao J. J., Gui L., Zhou G. Z., Ruan H. M., Li Z. Q., Gui J. F. 2007; Isolation and characterization of Scophthalmus maximus rhabdovirus. Dis Aquat Organ 74:95–105 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.000037
Loading
/content/journal/jgv/10.1099/vir.0.000037
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error