1887

Abstract

Over recent decades, metagenomic studies have expanded the number of newly described, often unclassified, viruses within the family . Using broad-spectrum circovirus and cyclovirus PCRs, we characterized a novel circo-like virus in mosquitoes from Germany whose main putative ORFs shared very low amino acid identity with those of previously characterized circoviruses and cycloviruses. Phylogenetic and genetic distance analysis revealed that this new virus species defined, together with previously described mosquito- and bat faeces-derived circo-like viruses, a different genus, tentatively called , within the family . We further demonstrated that viruses of the putative genus all shared a genomic organization that was unique among the family . Further investigations are needed to determine the host range, tissue tropism and transmission route(s). This report increases the current knowledge of the genetic diversity and evolution of the members of the family .

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.000036
2015-04-01
2024-10-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/96/4/915.html?itemId=/content/journal/jgv/10.1099/vir.0.000036&mimeType=html&fmt=ahah

References

  1. Becker N., Krüger A., Kuhn C., Plenge-Bönig A., Thomas S. M., Schmidt-Chanasit J., Tannich E. 2014; [Mosquitoes as vectors for exotic pathogens in Germany]. Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz 57:531–540 (in German) [View Article][PubMed]
    [Google Scholar]
  2. Biagini P., Bendinelli M., Hino S., Kakkola L., Mankertz A., Niel C. 2011; Family Circoviridae. In Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses pp. 343–346 Edited by King A. M. Q., Adams M. J., Carstens E. B., Lefkowitz E. J. London: Academic Press;
    [Google Scholar]
  3. Blinkova O., Rosario K., Li L., Kapoor A., Slikas B., Bernardin F., Breitbart M., Delwart E. 2009; Frequent detection of highly diverse variants of cardiovirus, cosavirus, bocavirus, and circovirus in sewage samples collected in the United States. J Clin Microbiol 47:3507–3513 [View Article][PubMed]
    [Google Scholar]
  4. Cheung A. K., Ng T. F., Lager K. M., Alt D. P., Delwart E. L., Pogranichniy R. M. 2014; Identification of a novel single-stranded circular DNA virus in pig feces. Genome Announc 2:e00347-14 [View Article][PubMed]
    [Google Scholar]
  5. Drummond A. J., Suchard M. A., Xie D., Rambaut A. 2012; Bayesian phylogenetics with BEAUti and the beast 1.7. Mol Biol Evol 29:1969–1973 [View Article][PubMed]
    [Google Scholar]
  6. Ge X., Li J., Peng C., Wu L., Yang X., Wu Y., Zhang Y., Shi Z. 2011; Genetic diversity of novel circular ssDNA viruses in bats in China. J Gen Virol 92:2646–2653 [View Article][PubMed]
    [Google Scholar]
  7. International Committee on Taxonomy of Viruses 2014; Taxonomy proposal code 2014.006cV. http://talk.ictvonline.org/files/proposals/taxonomy_proposals_vertebrate1/m/vert01/4936.aspx
  8. Li L., Kapoor A., Slikas B., Bamidele O. S., Wang C., Shaukat S., Masroor M. A., Wilson M. L., Ndjango J.-B. N.other authors 2010a; Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. J Virol 84:1674–1682 [View Article][PubMed]
    [Google Scholar]
  9. Li L., Victoria J. G., Wang C., Jones M., Fellers G. M., Kunz T. H., Delwart E. 2010b; Bat guano virome: predominance of dietary viruses from insects and plants plus novel mammalian viruses. J Virol 84:6955–6965 [View Article][PubMed]
    [Google Scholar]
  10. Mankertz A., Caliskan R., Hattermann K., Hillenbrand B., Kurzendoerfer P., Mueller B., Schmitt C., Steinfeldt T., Finsterbusch T. 2004; Molecular biology of porcine circovirus: analyses of gene expression and viral replication. Vet Microbiol 98:81–88 [View Article][PubMed]
    [Google Scholar]
  11. Muhire B. M., Varsani A., Martin D. P. 2014; sdt: a virus classification tool based on pairwise sequence alignment and identity calculation. PLoS One 9:e108277 [View Article][PubMed]
    [Google Scholar]
  12. Ng T. F., Willner D. L., Lim Y. W., Schmieder R., Chau B., Nilsson C., Anthony S., Ruan Y., Rohwer F., Breitbart M. 2011; Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes. PLoS One 6:e20579 [View Article][PubMed]
    [Google Scholar]
  13. Phan T. G., Luchsinger V., Avendaño L. F., Deng X., Delwart E. 2014; Cyclovirus in nasopharyngeal aspirates of Chilean children with respiratory infections. J Gen Virol 95:922–927 [View Article][PubMed]
    [Google Scholar]
  14. Rosario K., Duffy S., Breitbart M. 2012; A field guide to eukaryotic circular single-stranded DNA viruses: insights gained from metagenomics. Arch Virol 157:1851–1871 [View Article][PubMed]
    [Google Scholar]
  15. Smits S. L., Zijlstra E. E., van Hellemond J. J., Schapendonk C. M., Bodewes R., Schürch A. C., Haagmans B. L., Osterhaus A. D. 2013; Novel cyclovirus in human cerebrospinal fluid, Malawi, 2010–2011. Emerg Infect Dis 19:1511–1513 [View Article][PubMed]
    [Google Scholar]
  16. Smits S. L., Schapendonk C. M., van Beek J., Vennema H., Schürch A. C., Schipper D., Bodewes R., Haagmans B. L., Osterhaus A. D., Koopmans M. P. 2014; New viruses in idiopathic human diarrhea cases, The Netherlands. Emerg Infect Dis 20:1218–1222 [View Article][PubMed]
    [Google Scholar]
  17. Stewart M. E., Perry R., Raidal S. R. 2006; Identification of a novel circovirus in Australian ravens (Corvus coronoides) with feather disease. Avian Pathol 35:86–92 [View Article][PubMed]
    [Google Scholar]
  18. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  19. Tan V., van Doorn H. R., Nghia H. D., Chau T. T., Tu T. P., de Vries M., Canuti M., Deijs M., Jebbink M. F.other authors 2013; Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections. MBio 4:e00231–13 [View Article][PubMed]
    [Google Scholar]
  20. Zhang W., Li L., Deng X., Kapusinszky B., Delwart E. 2014; What is for dinner? Viral metagenomics of US store bought beef, pork, and chicken. Virology 468-470:303–310 [View Article][PubMed]
    [Google Scholar]
/content/journal/jgv/10.1099/vir.0.000036
Loading
/content/journal/jgv/10.1099/vir.0.000036
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error