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Journal of General Virology header logo Journal of General Virology

Volume 106, Issue 5

Functional analysis of in modulating infection of BmNPV through apoptosis pathways in domestic silkworm () Open Access

Abstract

The silkworm, , is a crucial model insect in agriculture and biological research. Tetraspanins, known for their effects in regulating cellular functions like cell signalling, adhesion, migration and diffusion, take on a crucial role in viral dynamics, influencing both viral spread and entry into host cells. In this study, a tetraspanin gene called from the silkworm genome was identified and investigated. Tissue profiles showed that has the highest transcription level in midgut, with a marked increase following viral infection. The immunofluorescence localization suggested that BmTsp.C is primarily distributed on the cell membrane. Additionally, overexpression of in BmN cells facilitated the proliferation of BmNPV. Meanwhile, siRNA-mediated knockdown of could inhibit viral proliferation. In addition, knockdown of .C at the individual level further validated the remarkable effect of during viral infestation. Furthermore, overexpression of could regulate the expression of apoptosis-related genes. Results from flow cytometry indicated a decrease in the number of apoptotic cells after overexpression of . Taken together, our results demonstrated that , as an important factor in the Tetraspanin-enriched microdomains, exerts a significant influence on the proliferation of BmNPV, most likely through the cellular apoptosis pathway.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): apoptosis , BmNPV , bombyx mori and tetraspanins
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31972616)
    • Principle Award Recipient: QiaolingZhao
  • Jiangsu Provincial Key Research and Development Program (Award BE2021346)
    • Principle Award Recipient: QiaolingZhao
  • Basic Research Program of Jiangsu Province (Award BK20190959)
    • Principle Award Recipient: DondxuShen
© 2025 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. The Microbiology Society waived the open access fees for this article.
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2025-05-09
2025-05-18
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Functional analysis of BmTsp.C in modulating infection of BmNPV through apoptosis pathways in domestic silkworm (Bombyx mori)
J. Gen. Virol. 106, 002098 (2025); https://doi.org/10.1099/jgv.0.002098
/content/journal/jgv/10.1099/jgv.0.002098
/content/journal/jgv/10.1099/jgv.0.002098
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