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Abstract

RNA structures that are functionally important are defined as -acting RNA elements because their functions cannot be compensated for in trans. The -acting RNA elements in the 3′ UTR of coronaviruses are important for replication; however, the mechanism linking the -acting RNA elements to their replication function remains to be established. In the present study, a comparison of the biological processes of the interactome and the replication efficiency between the 3′ UTR -acting RNA elements in coronaviruses, including severe acute respiratory syndrome coronavirus 2, suggests that (i) the biological processes, including translation, protein folding and protein stabilization, derived from the analysis of the -acting RNA element interactome and (ii) the architecture of the -acting RNA elements and their interactomes are highly correlated with coronavirus replication. In addition, alteration of the interactome using the compound 5-benzyloxygramine can cause reduced coronavirus replication, reinforcing the connection between -acting RNA elements and replication by interactome. Together, these results link -acting RNA elements to the coronavirus replication and establish a model to analyse the -acting RNA elements in the replication of RNA viruses by interactome.

Funding
This study was supported by the:
  • Chung Shan Medical University (Award NCHU-CSMU 11306)
    • Principle Award Recipient: WangMeilin
  • National Chung-Hsing University (Award NCHU-CSMU 11306)
    • Principle Award Recipient: WuHung-Yi
  • National Science and Technology Council (Award 111-2327-B-005-003 and 113-2313-B-005-009-MY3)
    • Principle Award Recipient: WuHung-Yi
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/content/journal/jgv/10.1099/jgv.0.002070
2025-01-30
2025-02-15
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