1887

Abstract

multiple nucleopolyhedrovirus (AcMNPV)-encoded microRNAs (miRNAs) that regulate viral genes to achieve infection have been reported previously. Here, we report another AcMNPV encoded miRNA, AcMNPV-miR-4 (Ac-miR-4), which downregulated the host gene, apoptosis-linked gene (). This regulation was verified by dual-luciferase reporter assays. The effects of Ac-miR-4 on virus infection were assessed. The results showed that the production of infectious budded virions (BV) was decreased and the occlusion-derived virion (ODV) embedding into polyhedra was delayed when Sf9 cells were administered an overdose of Ac-miR-4. All these findings suggest that Ac-miR-4 prolongs cell lifespan and reduces virus virulence at a relatively early stage but increases ODV at a very late stage. This finding may be attributed to the downregulation effects of , which lead to weakened ALG-2 related functions, such as cell apoptosis, vesicle budding and protein transport.

Keyword(s): AcMNPV , host gene , miRNA , regulation and target
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31872024)
    • Principle Award Recipient: JinwenWang
  • Natural Science Foundation of Guangdong Province (Award 2015A030313100)
    • Principle Award Recipient: JinwenWang
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/content/journal/jgv/10.1099/jgv.0.001769
2022-07-13
2024-10-09
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