1887

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Volume 103, Issue 4

The role of hepatitis B virus surface proteins in regulating the maturation and secretion of complete and incomplete virions Open Access

Abstract

The expression of various forms of hepatitis B virus (HBV) surface proteins regulates the release of mature virion, but whether they affect the release of other incomplete viral particles, such as naked capsid, is not clear. Here, by stable overexpression of large or middle/small hepatitis B surface proteins (LHBs, M/SHBs) in HepAD38 cells, we evaluated their effects on the release of complete and incomplete viral particles. Overproduction of LHBs inhibited the release of all surface proteins, which increased the ratio of naked capsids/virions. This effect was accompanied by the elevated extracellular HBV RNA. On the other hand, overexpression of M/SHBs greatly improved the secretion of enveloped viral and subviral particles. visualization of viral DNA and LHBs revealed intracellular retention of mature virions when LHBs were overexpressed. These results indicate that the molecular decision on secretion of enveloped or unenveloped viral particles is modulated by the intracellular ratio of large, middle and small surface antigens. This mechanism may be relevant in the progression and resolution of HBV-induced chronic liver disease.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): HBV RNA , HBV virions , LHBs , naked capsids and SHBs
Funding
This study was supported by the:
  • Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program (Award 2017BT01S131)
    • Principle Award Recipient: ZhenghongYuan
  • National Natural Science Foundation (Award 91842309)
  • National Natural Science Foundation (Award 91542207)
    • Principle Award Recipient: XiaonanZhang
  • National Natural Science Foundation (Award 81671998)
    • Principle Award Recipient: XiaonanZhang
  • National Natural Science Foundation (Award 81873962)
    • Principle Award Recipient: XiaonanZhang
  • National SCience and Technology Major project of China (Award 2017ZX10302201001005)
    • Principle Award Recipient: XiaonanZhang
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-04-19
2024-04-25
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The role of hepatitis B virus surface proteins in regulating the maturation and secretion of complete and incomplete virions
J. Gen. Virol. 103, 001733 (2022); https://doi.org/10.1099/jgv.0.001733
/content/journal/jgv/10.1099/jgv.0.001733
/content/journal/jgv/10.1099/jgv.0.001733
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