1887

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Volume 103, Issue 2

Role of gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus No Access

Abstract

Avian leukosis virus (ALV) is a retrovirus that induces tumours in infected birds; ALV is divided into different subgroups according to the gene and cellular tropism. In general, ALV subgroup J (ALV-J) is considered to be the most pathogenic and prevalent subgroup while subgroup K (ALV-K), a newly identified subgroup, only causes mild symptoms. To illuminate the roles of the viral gene and LTR sequence in pathogenic differences between ALV-J and ALV-K, rescued ALV-J strain rSDAU1005, rescued ALV-K strain rJS11C1, and recombinant strains rENV(J)-LTR(K) and rENV(K)-LTR(J) were characterized and investigated in this study. Among rescued viruses, rSDAU1005 had the highest replication efficiency while rJS11C1 replicated the slowest (replication efficiency rankings were rSDAU1005 >rENV(K)-LTR(J)>rENV(J)-LTR(K)>rJS11 C1). The luciferase reporter gene assay results showed that the promoter activity of ALV-K LTR was lower than that of the ALV-J LTR promoter, which may have accounted for the slower replication efficiency of ALV-K. Pathogenicity of the four rescued viruses was determined via inoculating the yolk sacs of specific-pathogen-free chickens. The results demonstrated that all four viruses were pathogenic; rSDAU1005 caused the most severe growth retardation and immunosuppression. rENV(J)-LTR(K) was more pathogenic when compared to rENV(K)-LTR(J), indicating that and the LTR sequence play important roles in pathogenicity between ALV-K and ALV-J. Additionally, seemed to especially play a role in ALV-K pathogenesis. This study provided scientific data and insight to improve detection methods and judgement criteria in ALV clearance and surveillance.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): envgene , LTR , pathogenicity , recombinant chimeric virus , replication and subgroup K avian leukosis virus
Funding
This study was supported by the:
  • Shandong “Double Tops” Program (Award 2017003)
    • Principle Award Recipient: YixinWang
  • Postdoctoral Research Foundation of China (Award 2018M642686)
    • Principle Award Recipient: YixinWang
  • Natural Science Foundation of Shandong Province (Award ZR2018BC047)
    • Principle Award Recipient: YixinWang
© 2022 The Authors
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/content/journal/jgv/10.1099/jgv.0.001719
2022-02-07
2024-04-20
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Role of env gene and LTR sequence in the pathogenesis of subgroup K avian leukosis virus
J. Gen. Virol. 103, 001719 (2022); https://doi.org/10.1099/jgv.0.001719
/content/journal/jgv/10.1099/jgv.0.001719
/content/journal/jgv/10.1099/jgv.0.001719
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