@article{mbs:/content/journal/jgv/10.1099/jgv.0.001706, author = "Tochetto, Caroline and Cibulski, Samuel Paulo and Muterle Varela, Ana Paula and Cerva, Cristine and Alves de Lima, Diane and Fumaco Teixeira, Thais and Quoos Mayer, Fabiana and Roehe, Paulo Michel", title = "A variety of highly divergent eukaryotic ssDNA viruses in sera of pigs", journal= "Journal of General Virology", year = "2021", volume = "102", number = "12", pages = "", doi = "https://doi.org/10.1099/jgv.0.001706", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001706", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "CRESS DNA", keywords = "high-throughput sequencing", keywords = "virome", keywords = "metagenomics", keywords = "swine", eid = "001706", abstract = "Over the last decade, viral metagenomics has been established as a non-targeted approach for identifying viruses in stock animals, including pigs. This has led to the identification of a vast diversity of small circular ssDNA viruses. The present study focuses on the investigation of eukaryotic circular Rep-encoding single-stranded (CRESS) DNA viral genomes present in serum of commercially reared pigs from southern Brazil. Several CRESS DNA viral genomes were detected, including representatives of the families Smacoviridae (n=5), Genomoviridae (n=3), Redondoviridae (n=1), Nenyaviridae (n=1) and other yet unclassified genomes (n=9), plus a circular DNA molecule, which probably belongs to the phylum Cressdnaviricota. A novel genus within the family Smacoviridae, tentatively named ‘Suismacovirus’, comprising 21 potential new species, is proposed. Although the reported genomes were recovered from pigs with clinical signs of respiratory disease, further studies should examine their potential role as pathogens. Nonetheless, these findings highlight the diversity of circular ssDNA viruses in serum of domestic pigs, expand the knowledge on CRESS DNA viruses’ genetic diversity and distribution and contribute to the global picture of the virome of commercially reared pigs.", }