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Abstract

Over the last decade, viral metagenomics has been established as a non-targeted approach for identifying viruses in stock animals, including pigs. This has led to the identification of a vast diversity of small circular ssDNA viruses. The present study focuses on the investigation of eukaryotic circular Rep-encoding single-stranded (CRESS) DNA viral genomes present in serum of commercially reared pigs from southern Brazil. Several CRESS DNA viral genomes were detected, including representatives of the families (=5), (=3), (=1), (=1) and other yet unclassified genomes (=9), plus a circular DNA molecule, which probably belongs to the phylum . A novel genus within the family tentatively named ‘’, comprising 21 potential new species, is proposed. Although the reported genomes were recovered from pigs with clinical signs of respiratory disease, further studies should examine their potential role as pathogens. Nonetheless, these findings highlight the diversity of circular ssDNA viruses in serum of domestic pigs, expand the knowledge on CRESS DNA viruses’ genetic diversity and distribution and contribute to the global picture of the virome of commercially reared pigs.

Funding
This study was supported by the:
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 309024/2020-0)
    • Principle Award Recipient: PauloMichel Roehe
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award 88887.364979/2019-00)
    • Principle Award Recipient: CarolineTochetto
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award 88882.181808/2018-01)
    • Principle Award Recipient: CarolineTochetto
  • Financiadora de Estudos e Projetos (Award 01.12.0113.00)
    • Principle Award Recipient: NotApplicable
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 421104/2018-0)
    • Principle Award Recipient: PauloMichel Roehe
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/content/journal/jgv/10.1099/jgv.0.001706
2021-12-20
2022-01-28
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