RT Journal Article SR Electronic(1) A1 Khan, Haris Ahmed A1 Shamsi, Wajeeha A1 Jamal, Atif A1 Javaied, Memoona A1 Sadiq, Mashal A1 Fatma, Tehsin A1 Ahmed, Aqeel A1 Arshad, Maleeha A1 Waseem, Mubashra A1 Babar, Samra A1 Dogar, Midhat Mustafa A1 Virk, Nasar A1 Janjua, Hussnain Ahmed A1 Kondo, Hideki A1 Suzuki, Nobuhiro A1 Bhatti, Muhammad FarazYR 2021 T1 Assessment of mycoviral diversity in Pakistani fungal isolates revealed infection by 11 novel viruses of a single strain of Fusarium mangiferae isolate SP1 JF Journal of General Virology, VO 102 IS 12 OP SP 001690 DO https://doi.org/10.1099/jgv.0.001690 PB Microbiology Society, SN 1465-2099, AB An extensive screening survey was conducted on Pakistani filamentous fungal isolates for the identification of viral infections. A total of 396 fungal samples were screened, of which 36 isolates were found double-stranded (ds) RNA positive with an overall frequency of 9% when analysed by a classical dsRNA isolation method. One of 36 dsRNA-positive strains, strain SP1 of a plant pathogenic fungus Fusarium mangiferae, was subjected to virome analysis. Next-generation sequencing and subsequent completion of the entire genome sequencing by a classical Sanger sequencing method showed the SP1 strain to be co-infected by 11 distinct viruses, at least seven of which should be described as new taxa at the species level according to the ICTV (International Committee on Taxonomy of Viruses) species demarcation criteria. The newly identified F. mangiferae viruses (FmVs) include two partitivirids, one betapartitivirus (FmPV1) and one gammapartitivirus (FmPV2); six mitovirids, three unuamitovirus (FmMV2, FmMV4, FmMV6), one duamitovirus (FmMV5), and two unclassified mitovirids (FmMV1, FmMV3); and three botourmiavirids, two magoulivirus (FmBOV1, FmBOV3) and one scleroulivirus (FmBOV2). The number of coinfecting viruses is among the largest ones of fungal coinfections. Their molecular features are thoroughly described here. This represents the first large virus survey in the Indian sub-continent., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001690