@article{mbs:/content/journal/jgv/10.1099/jgv.0.001656, author = "Pérez-Cañamás, Miryam and Hevia, Elizabeth and Katsarou, Konstantina and Hernández, Carmen", title = "Genetic evidence for the involvement of Dicer-like 2 and 4 as well as Argonaute 2 in the Nicotiana benthamiana response against Pelargonium line pattern virus", journal= "Journal of General Virology", year = "2021", volume = "102", number = "10", pages = "", doi = "https://doi.org/10.1099/jgv.0.001656", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001656", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "antiviral RNA silencing", keywords = "DCL", keywords = "Nicotiana benthamiana", keywords = "RNA virus", keywords = "PLPV", keywords = "AGO2", eid = "001656", abstract = "In plants, RNA silencing functions as a potent antiviral mechanism. Virus-derived double-stranded RNAs (dsRNAs) trigger this mechanism, being cleaved by Dicer-like (DCL) enzymes into virus small RNAs (vsRNAs). These vsRNAs guide sequence-specific RNA degradation upon their incorporation into an RNA-induced silencing complex (RISC) that contains a slicer of the Argonaute (AGO) family. Host RNA dependent-RNA polymerases, particularly RDR6, strengthen antiviral silencing by generating more dsRNA templates from RISC-cleavage products that, in turn, are converted into secondary vsRNAs by DCLs. Previous work showed that Pelargonium line pattern virus (PLPV) is a very efficient inducer and target of RNA silencing as PLPV-infected Nicotiana benthamiana plants accumulate extraordinarily high amounts of vsRNAs that, strikingly, are independent of RDR6 activity. Several scenarios may explain these observations including a major contribution of dicing versus slicing for defence against PLPV, as the dicing step would not be affected by the RNA silencing suppressor encoded by the virus, a protein that acts via vsRNA sequestration. Taking advantage of the availability of lines of N. benthamiana with DCL or AGO2 functions impaired, here we have tried to get further insights into the components of the silencing machinery that are involved in anti-PLPV-silencing. Results have shown that DCL4 and, to lesser extent, DCL2 contribute to restrict viral infection. Interestingly, AGO2 apparently makes even a higher contribution in the defence against PLPV, extending the number of viruses that are affected by this particular slicer. The data support that both dicing and slicing activities participate in the host race against PLPV.", }