1887

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Volume 102, Issue 8

A virulent and pathogenic infectious clone of Senecavirus A No Access

Abstract

Senecavirus A (SVA) is a picornavirus that circulates in swine populations worldwide causing vesicular disease (VD) in affected animals. Here we developed a reverse genetics system for SVA based on the well-characterized wild-type SVA strain SD15-26 (wt SVA SD15-26). The full-length cDNA genome of SVA was cloned into a plasmid under a T7 RNA polymerase promoter. Following transcription, the genomic viral RNA was transfected into BHK-21 cells and rescue of infectious virus (rSVA SD15-26) was shown by inoculation of highly susceptible H1299 cells. characterization of the rSVA SD15-26 showed similar replication properties and protein expression levels as the wt SVA SD15-26. A pathogenesis study was conducted in 15-week-old finishing pigs to evaluate the pathogenicity and infection dynamics of the rSVA SD15-26 virus in comparison to the wt SVA SD15-26. Animals from both rSVA- and wt SVA SD15-26-inoculated groups presented characteristic SVA clinical signs (lethargy and lameness) followed by the development of vesicular lesions on the snout and/or feet. The clinical outcome of infection, including disease onset, severity and duration was similar in rSVA- and the wt SVA SD15-26-inoculated animals. All animals inoculated with rSVA or with wt SVA SD15-26 presented a short-term viremia, and animals from both groups shed similar amounts of virus in oral and nasal secretion, and faeces. Our data demonstrates that the rSVA SD5-26 clone is fully virulent and pathogenic in pigs, presenting comparable pathogenesis and infection dynamics to the wt SVA SD15-26 strain. The infectious clone generated here is a useful platform to study virulence determinants of SVA, and to dissect other aspects of SVA infection biology, pathogenesis and persistence.

  • Received:
  • Accepted:
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Keyword(s): infectious clone , recombinant , reverse genetics , Seneca Valley virus , SVA , SVV and vesicular disease
Funding
This study was supported by the:
  • National Institute of Food and Agriculture (Award 2019-67015-29830)
    • Principle Award Recipient: G. DielDiego
© 2021 The Authors
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/content/journal/jgv/10.1099/jgv.0.001643
2021-08-23
2024-04-19
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References

  1. Bracht AJ, O’Hearn ES, Fabian AW, Barrette RW, Sayed A. Real-Time reverse transcription PCR assay for detection of Senecavirus A in swine Vesicular diagnostic specimens. PLoS One 2016; 11:e0146211 [View Article] [PubMed]
     [Google Scholar]
  2. Joshi LR, Mohr KA, Clement T, Hain KS, Myers B et al. Detection of the emerging picornavirus Senecavirus A in Pigs. J Clin Microbiol 2016; 54:1536–1545 [View Article]
     [Google Scholar]
  3. Hause BM, Myers O, Duff J, Hesse RA. Senecavirus A in pigs, United States, 2015. Emerg Infect Dis 2016; 22:1323–1325 [View Article] [PubMed]
     [Google Scholar]
  4. Wu Q, Zhao X, Bai Y, Sun B, Xie Q et al. The first identification and complete genome of Senecavirus A affecting pig with idiopathic vesicular disease in China. Transbound Emerg Dis 2017; 64:1633–1640 [View Article] [PubMed]
     [Google Scholar]
  5. Saeng-Chuto K, Stott CJ, Wegner M, Kaewprommal P, Piriyapongsa J et al. The full-length genome characterization, genetic diversity and evolutionary analyses of Senecavirus A isolated in Thailand in 2016. Infect Genet Evol 2018; 64:32–45 [View Article] [PubMed]
     [Google Scholar]
  6. Sun D, Vannucci F, Knutson TP, Corzo C, Marthaler DG. Emergence and whole-genome sequence of Senecavirus A in Colombia. Transbound Emerg Dis 2017; 64:1346–1349 [View Article] [PubMed]
     [Google Scholar]
  7. Arzt J, Bertram MR, Vu LT, Pauszek SJ, Hartwig EJ et al. First detection and genome sequence of Senecavirus A in Vietnam. Microbiol Resour Announc 2019; 8: [View Article]
     [Google Scholar]
  8. Leme RA, Zotti E, Alcântara BK, Oliveira MV, Freitas LA et al. Senecavirus A: An emerging vesicular infection in Brazilian pig herds. Transbound Emerg Dis 2015; 62:603–611 [View Article] [PubMed]
     [Google Scholar]
  9. Pasma T, Davidson S, Shaw SL. Idiopathic vesicular disease in swine in Manitoba. Can Vet J = La Rev Vet Can 2008; 49:84–85
     [Google Scholar]
  10. Knight-Jones TJD, Rushton J. The economic impacts of foot and mouth disease - what are they, how big are they and where do they occur?. Prev Vet Med 2013; 112:161–173 [View Article] [PubMed]
     [Google Scholar]
  11. Joshi LR, Fernandes MHV, Clement T, Lawson S, Pillatzki A et al. Pathogenesis of Senecavirus A infection in finishing pigs. J Gen Virol 2016; 97:3267–3279 [View Article] [PubMed]
     [Google Scholar]
  12. Maggioli MF, Lawson S, de Lima M, Joshi LR, Faccin TC et al. Adaptive immune responses following Senecavirus A infection in Pigs. J Virol 2018; 92: [View Article] [PubMed]
     [Google Scholar]
  13. Fernandes MHV, Maggioli MF, Joshi LR, Clement T, Faccin TC et al. Pathogenicity and cross-reactive immune responses of a historical and a contemporary Senecavirus A strains in pigs. Virology 2018; 522:147–157 [View Article] [PubMed]
     [Google Scholar]
  14. Maggioli MF, Fernandes MHV, Joshi LR, Sharma B, Tweet MM et al. Persistent infection and transmission of Senecavirus A from carrier sows to contact piglets. J Virol 2019; 93: [View Article] [PubMed]
     [Google Scholar]
  15. Hales LM, Knowles NJ, Reddy PS, Xu L, Hay C et al. Complete genome sequence analysis of Seneca Valley virus-001, a novel oncolytic picornavirus. J Gen Virol 2008; 89:1265–1275 [View Article] [PubMed]
     [Google Scholar]
  16. Venkataraman S, Reddy SP, Loo J, Idamakanti N, Hallenbeck PL et al. Structure of Seneca Valley Virus-001: an oncolytic picornavirus representing a new genus. Structure 2008; 16:1555–1561 [View Article] [PubMed]
     [Google Scholar]
  17. Willcocks MM, Locker N, Gomwalk Z, Royall E, Bakhshesh M et al. Structural features of the Seneca Valley virus internal ribosome entry site (IRES) element: a picornavirus with a pestivirus-like IRES. J Virol 2011; 85:4452–4461 [View Article] [PubMed]
     [Google Scholar]
  18. Zoll J, Heus HA, van Kuppeveld FJM, Melchers WJG. The structure-function relationship of the enterovirus 3’-UTR. Virus Res 2009; 139:209–216 [View Article] [PubMed]
     [Google Scholar]
  19. Rohll JB, Moon DH, Evans DJ, Almond JW. The 3’ untranslated region of picornavirus RNA: features required for efficient genome replication. J Virol 1995; 69:7835–7844 [View Article]
     [Google Scholar]
  20. Kloc A, Rai DK, Rieder E. The roles of picornavirus untranslated regions in infection and innate immunity. Front Microbiol 2018; 9:485 [View Article] [PubMed]
     [Google Scholar]
  21. Lin JY, Chen TC, Weng KF, Chang SC, Chen L-L et al. Viral and host proteins involved in picornavirus life cycle. J Biomed Sci 2009; 16:103 [View Article] [PubMed]
     [Google Scholar]
  22. Porter AG. Picornavirus nonstructural proteins: emerging roles in virus replication and inhibition of host cell functions. J Virol 1993; 67:6917–6921 [View Article] [PubMed]
     [Google Scholar]
  23. Wang Y, Ma L, Stipkovits L, Szathmary S, Li X. The strategy of picornavirus evading host antiviral responses: Nonstructural proteins suppress the production of IFNs. Front Microbiol 2018; 9:2943 [View Article] [PubMed]
     [Google Scholar]
  24. Stobart CC, Moore ML. RNA virus reverse genetics and vaccine design. Viruses 2014; 6:2531–2550 [View Article] [PubMed]
     [Google Scholar]
  25. Sharma B, Fernandes MH, De Lima M, Joshi LR, Lawson S et al. A Novel Live Attenuated Vaccine Candidate Protects Against Heterologous Senecavirus A Challenge. Front Immunol 2019; 10:2660 [View Article] [PubMed]
     [Google Scholar]
  26. Chen Z, Yuan F, Li Y, Shang P, Schroeder R et al. Construction and characterization of a full-length cDNA infectious clone of emerging porcine Senecavirus A. Virology 2016; 497:111–124 [View Article] [PubMed]
     [Google Scholar]
  27. Liu F, Huang Y, Wang Q, Shan H. Construction of eGFP-Tagged senecavirus a for facilitating virus neutralization test and antiviral assay. Viruses 2020; 12: [View Article] [PubMed]
     [Google Scholar]
  28. Wang M, Mou C, Chen Z, Chen Z. Infectious recombinant Senecavirus A expressing novel reporter proteins. Appl Microbiol Biotechnol 2021; 105:2385–2397 [View Article] [PubMed]
     [Google Scholar]
  29. Li C, Wang H, Shi J, Yang D, Zhou G et al. Senecavirus-Specific recombination assays reveal the intimate link between Polymerase fidelity and RNA recombination. J Virol 2019; 93: [View Article]
     [Google Scholar]
  30. Hierholzer JC, Killington RA. Virus isolation and quantitation. Mahy B, Kangro H. eds In Virology Methods manual San Diego: Academic Press; 1996 pp 25–47
     [Google Scholar]
  31. Fernandes MHV, Maggioli MF, Otta J, Joshi LR, Lawson S et al. Senecavirus A 3C protease mediates host cell apoptosis late in infection. Front Immunol 2019; 10:363 [View Article] [PubMed]
     [Google Scholar]
  32. Maggioli MF, Lawson S, de Lima M, Joshi LR, Faccin TC et al. Adaptive Immune Responses following Senecavirus A Infection in Pigs. J Virol 2018; 92:e01717 [View Article]
     [Google Scholar]
  33. Knowles NJ, Hales LM, Jones BH, Landgraf JG, House JA et al. Epidemiology of Seneca valley virus: identification and characterization of isolates from pigs in the United States. In XIVth Meeting of the European Study Group on Molecular Biology of Picornaviruses Saariselka: 2006
     [Google Scholar]
  34. Sun C, Yang D, Gao R, Liang T, Wang H. Modification of the internal ribosome entry site element impairs the growth of foot-and-mouth disease virus in porcine-derived cells. J Gen Virol 2016; 97:901–911 [View Article] [PubMed]
     [Google Scholar]
  35. García-Nuñez S, Gismondi MI, König G, Berinstein A, Taboga O et al. Enhanced IRES activity by the 3’UTR element determines the virulence of FMDV isolates. Virology 2014; 448:303–313 [View Article] [PubMed]
     [Google Scholar]
  36. Li R, Zou Q, Chen L, Zhang H, Wang Y. Molecular analysis of virulent determinants of enterovirus 71. PLOS One 2011; 6:26237
     [Google Scholar]
  37. Guo B, Piñeyro PE, Rademacher CJ, Zheng Y, Li G. Novel senecavirus a in swine with vesicular disease, United States, July 2015. Emerg Infect Dis 2016; 22:1325–1327 [View Article] [PubMed]
     [Google Scholar]
  38. Montiel N, Buckley A, Guo B, Kulshreshtha V, VanGeelen A et al. Vesicular disease in 9-Week-Old pigs experimentally infected with Senecavirus A. Emerg Infect Dis 2016; 22:1246–1248 [View Article] [PubMed]
     [Google Scholar]
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A virulent and pathogenic infectious clone of Senecavirus A
J. Gen. Virol. 102, 001643 (2021); https://doi.org/10.1099/jgv.0.001643
/content/journal/jgv/10.1099/jgv.0.001643
/content/journal/jgv/10.1099/jgv.0.001643
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