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Abstract

The RNA-dependent RNA polymerase (1E) is involved in replication of grapevine fanleaf virus (GFLV, , ) and causes vein clearing symptoms in . Information on protein 1E interaction with other viral and host proteins is scarce. To study protein 1E biology, three GFLV infectious clones, i.e. GHu (a symptomatic wild-type strain), GHu-1E (an asymptomatic GHu mutant) and F13 (an asymptomatic wild-type strain), were engineered with protein 1E fused to a V5 epitope tag at the C-terminus. Following -mediated delivery of GFLV clones in and protein extraction at seven dpi, when optimal 1E:V5 accumulation was detected, two viral and six plant putative interaction partners of V5-tagged protein 1E were identified for the three GFLV clones by affinity purification and tandem mass spectrometry. This study provides insights into the protein interactome of 1E during GFLV systemic infection in and lays the foundation for validation work.

Funding
This study was supported by the:
  • USDA-ARS-CRIS (Award 8062-22410-007-00-D)
    • Principle Award Recipient: MichelleHeck
  • Cornell AgriTech Venture Capital Funds
    • Principle Award Recipient: MarcF Fuchs
  • USDA-AFRI-NIFA (Award 2018-67011-28107)
    • Principle Award Recipient: LarissaJ. Osterbaan
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-05-27
2022-01-24
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