1887

Abstract

RNA-remodelling proteins, including RNA helicases and chaperones, function to remodel structured RNAs and/or RNA–protein interactions and play indispensable roles in viral life cycles. Guaico Culex virus (GCXV) is the first uncovered animal-infected multicomponent virus with segmented positive-sense genomic RNAs. GCXV belongs to the Jingmenvirus group, a diverse clade of segmented viruses that are related to the prototypically unsegmented . However, little is known about the exact functions of the GCXV-encoded proteins. Here, we show that the putative non-structural protein (NSP) 2 on segment 2 of GCXV functions as an RNA helicase that unwinds RNA helix bidirectionally in an adenosine triphosphate (ATP)-dependent manner, and an RNA chaperone that remodels structured RNAs and facilitates RNA strand annealing independently of ATP. Together, our findings are the first demonstration of RNA-remodelling activity encoded by Jingmenvirus and highlight the functional significance of NSP2 in the GCXV life cycle.

Funding
This study was supported by the:
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences (Award 2020332)
    • Principle Award Recipient: YangQiu
  • National Natural Science Foundation of China (Award 31970169)
    • Principle Award Recipient: xizhou
  • National Natural Science Foundation of China (Award 81873964)
    • Principle Award Recipient: YangQiu
  • Strategic Priority Research Program of CAS (Award XDB29010300)
    • Principle Award Recipient: xizhou
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2021-04-23
2021-05-15
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