1887

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Volume 102, Issue 3

Different potato virus Y strains frequently co-localize in single epidermal leaf cells and in the aphid stylet Free

Abstract

Single aphids can simultaneously or sequentially acquire and transmit multiple potato virus Y (PVY) strains. Multiple PVY strains are often found in the same field and occasionally within the same plant, but little is known about how PVY strains interact in plants or in aphid stylets. Immuno-staining and confocal microscopy were used to examine the spatial and temporal dynamics of PVY strain mixtures (PVY and PVY or PVY and PVY) in epidermal leaf cells of ‘Samsun NN’ tobacco and ‘Goldrush’ potato. Virus binding and localization was also examined in aphid stylets following acquisition. Both strains systemically infected tobacco and co-localized in cells of all leaves examined; however, the relative amounts of each virus changed over time. Early in the tobacco infection, when mosaic symptoms were observed, PVY dominated the infection although PVY was detected in some cells. As the infection progressed and vein necrosis developed, PVY was prevalent. Co-localization of PVY and PVY was also observed in epidermal cells of potato leaves with most cells infected with both viruses. Furthermore, two strains could be detected binding to the distal end of aphid stylets following virus acquisition from a plant infected with a strain mixture. These data are in contrast with the traditional belief of spatial separation of two closely related potyviruses and suggest apparent non-antagonistic interaction between PVY strains that could help explain the multitude of emerging recombinant PVY strains discovered in potato in recent years.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): confocal microscopy , Myzus persicae stylet , potato , PVY , recombinant strain and strain interaction
Funding
This study was supported by the:
  • USDA-NSF-BBSRC Ecology and Evolution of Infectious Diseases program grant (Award 2013-04567)
    • Principle Award Recipient: StewartM. Gray
  • USDA-SCRI (Award 2014-51181-22373)
    • Principle Award Recipient: StewartM. Gray
  • USDA-SCRI (Award 2009-51181-05894)
    • Principle Award Recipient: StewartM. Gray
© Microbiology Society
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2021-03-12
2024-04-25
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Different potato virus Y strains frequently co-localize in single epidermal leaf cells and in the aphid stylet
J. Gen. Virol. 102, 001576 (2021); https://doi.org/10.1099/jgv.0.001576
/content/journal/jgv/10.1099/jgv.0.001576
/content/journal/jgv/10.1099/jgv.0.001576
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