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Volume 102, Issue 3

Zebrafish as an animal model for the antiviral RNA interference pathway Free

Abstract

The zebrafish () possesses evolutionarily conserved innate and adaptive immunity as a mammal and has recently become a popular vertebrate model to exploit infection and immunity. Antiviral RNA interference (RNAi) has been illuminated in various model organisms, including , , and mice. However, to date, there is no report on the antiviral RNAi pathway of zebrafish. Here, we have evaluated the possible use of zebrafish to study antiviral RNAi with Sindbis virus (SINV), vesicular stomatitis virus (VSV) and Nodamura virus (NoV). We find that SINVs and NoVs induce the production of virus-derived small interfering RNAs (vsiRNAs), the hallmark of antiviral RNAi, with a preference for a length of 22 nucleotides, after infection of larval zebrafish. Meanwhile, the suppressor of RNAi (VSR) protein, NoV B2, may affect the accumulation of the NoV in zebrafish. Furthermore, taking advantage of the fact that zebrafish argonaute-2 (Ago2) protein is naturally deficient in cleavage compared with that of mammals, we provide evidence that the slicing activity of human Ago2 can virtually inhibit the accumulation of RNA virus after being ectopically expressed in larval zebrafish. Thus, zebrafish may be a unique model organism to study the antiviral RNAi pathway.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antiviral response , antiviral RNA interference , argonaute-2 protein , RNA virus and zebrafish
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31770179)
    • Principle Award Recipient: yangli
  • National Natural Science Foundation of China (Award 91640111)
    • Principle Award Recipient: yangli
© 2021 The Authors
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/content/journal/jgv/10.1099/jgv.0.001552
2021-01-28
2024-04-19
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Zebrafish as an animal model for the antiviral RNA interference pathway
J. Gen. Virol. 102, 001552 (2021); https://doi.org/10.1099/jgv.0.001552
/content/journal/jgv/10.1099/jgv.0.001552
/content/journal/jgv/10.1099/jgv.0.001552
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