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Abstract

Rotavirus A (RVA) is a major diarrhoea-causing pathogen in young animals and children. The zoonotic potential of RVA has received extensive attention in recent years. In May 2018, an outbreak of diarrhoea among piglets occurred on a swine farm in Sichuan province, PR China. RVA was detected in 95.7 % (22/23) of piglet samples, 60 % (9/15) of sow samples and 100 % (3/3) of pig-breeder faecal samples. The predominant RVA genotype on this swine farm was G3P[13], and G3P[13] RVA was also detected in the three breeder faecal samples. Three G3P[13] RVA strains were isolated from a piglet faecal sample, a sow faecal sample and a pig-breeder faecal sample, and were named SCLS-X1, SCLS-3 and SCLS-R3, respectively. The complete sequences of 11 gene segments of these three isolates were derived. Phylogenetic analysis showed that ten gene segments (VP7, VP4, VP1–VP3 and NSP1–NSP5) of pig-breeder isolate SCLS-R3 were closely related to pig isolates SCLS-X1 and SCLS-3 from this farm. Only the VP6 gene shared higher homology with human RVA strain I321. Therefore, a G3P[13] porcine RVA strain most likely infected pig breeders. These results provided the first complete epidemiological link demonstrating interspecies transmission of G3P[13] RVA from pigs to human. Our data contribute to an improved understanding of the genetic evolution and interspecies transmission of RVA.

Funding
This study was supported by the:
  • the Innovation Team of Beef Cattle, Sichuan Province
    • Principle Award Recipient: ChengTang
  • the innovation team for emerging animal diseases on Qinghai-Tibet Plateau, Southwest Minzu University (Award 2020NTD02)
    • Principle Award Recipient: ChengTang
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/content/journal/jgv/10.1099/jgv.0.001532
2020-12-09
2021-10-25
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References

  1. Estes MK, Greenberg HB. Rotaviruses. In Knipe DM, Howley PM. (editors) Fields Virology, 6th ed. Philadelphia: Lippincott Williams & Wilkins; 2013 pp 1347–1401
    [Google Scholar]
  2. Glass RI, Parashar UD, Bresee JS, Turcios R, Fischer TK et al. Rotavirus vaccines: current prospects and future challenges. Lancet 2006; 368:323–332 [View Article][PubMed]
    [Google Scholar]
  3. Bányai K, László B, Duque J, Steele AD, Nelson EA et al. Systematic review of regional and temporal trends in global rotavirus strain diversity in the pre rotavirus vaccine era: insights for understanding the impact of rotavirus vaccination programs. Vaccine 2012; 30:A122–A130 [View Article][PubMed]
    [Google Scholar]
  4. Komoto S, Tacharoenmuang R, Guntapong R, Ide T, Sinchai P et al. Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission. J Gen Virol 2017; 98:532–538 [View Article][PubMed]
    [Google Scholar]
  5. Ghosh S, Kobayashi N. Exotic rotaviruses in animals and rotaviruses in exotic animals. Virusdisease 2014; 25:158–172 [View Article][PubMed]
    [Google Scholar]
  6. Palombo EA, Clark R, Bishop RF. Characterisation of a "European-like" serotype G8 human rotavirus isolated in Australia. J Med Virol 2000; 60:56–62 [View Article][PubMed]
    [Google Scholar]
  7. Park S, Matthijnssens J, Saif LJ, Kim HJ, Park JG et al. Reassortment among bovine, porcine and human rotavirus strains results in G8P[7] and G6P[7] strains isolated from cattle in South Korea. Vet Microbiol 2011; 152:55–66 [View Article][PubMed]
    [Google Scholar]
  8. Steyer A, Poljšak-Prijatelj M, Barlič-Maganja D, Marin J. Human, porcine and bovine rotaviruses in Slovenia: evidence of interspecies transmission and genome reassortment. J Gen Virol 2008; 89:1690–1698 [View Article][PubMed]
    [Google Scholar]
  9. Mukherjee A, Mullick S, Kobayashi N, Chawla-Sarkar M. The first identification of rare human group A rotavirus strain G3P[10] with severe infantile diarrhea in eastern India. Infect Genet Evol 2012; 12:1933–1937 [View Article][PubMed]
    [Google Scholar]
  10. Vlasova AN, Amimo JO, Saif LJ. Porcine rotaviruses: epidemiology, immune responses and control strategies. Viruses 2017; 9:48 [View Article][PubMed]
    [Google Scholar]
  11. Ward ML, Mijatovic-Rustempasic S, Roy S, Rungsrisuriyachai K, Boom JA et al. Molecular characterization of the first G24P[14] rotavirus strain detected in humans. Infect Genet Evol 2016; 43:338–342 [View Article][PubMed]
    [Google Scholar]
  12. Dóró R, Farkas SL, Martella V, Bányai K. Zoonotic transmission of rotavirus: surveillance and control. Expert Rev Anti Infect Ther 2015; 13:1337–1350 [View Article][PubMed]
    [Google Scholar]
  13. Matthijnssens J, Heylen E, Zeller M, Rahman M, Lemey P et al. Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 2010; 27:2431–2436 [View Article][PubMed]
    [Google Scholar]
  14. Cañon Jones H, Cortes H, Gaggero A, Levican J, Castillo-Ruiz M et al. High genetic diversity of species a rotaviruses detected in swine farms in Chile. J Gen Virol 2017; 98:539–547 [View Article][PubMed]
    [Google Scholar]
  15. Ennima I, Sebbar G, Harif B, Amzazi S, Loutfi C et al. Isolation and identification of group A rotaviruses among neonatal diarrheic calves, Morocco. BMC Res Notes 2016; 9:261 [View Article][PubMed]
    [Google Scholar]
  16. Sato K, Inaba Y, Miura Y, Tokuhisa S, Matumoto M. Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence. Arch Virol 1982; 73:45–50 [View Article][PubMed]
    [Google Scholar]
  17. Wang Y, Zhang J, Liu P. Clinical and molecular epidemiologic trends reveal the important role of rotavirus in adult infectious gastroenteritis, in Shanghai, China. Infect Genet Evol 2017; 47:143–154 [View Article][PubMed]
    [Google Scholar]
  18. Trojnar E, Sachsenröder J, Twardziok S, Reetz J, Otto PH et al. Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses. J Gen Virol 2013; 94:136–142 [View Article][PubMed]
    [Google Scholar]
  19. Wu FT, Bányai K, Jiang B, Liu LT, Marton S et al. Novel G9 rotavirus strains co-circulate in children and pigs, Taiwan. Sci Rep 2017; 7:40731 [View Article][PubMed]
    [Google Scholar]
  20. Hu L, Crawford SE, Czako R, Cortes-Penfield NW, Smith DF et al. Cell attachment protein VP8* of a human rotavirus specifically interacts with A-type histo-blood group antigen. Nature 2012; 485:256–259 [View Article][PubMed]
    [Google Scholar]
  21. Sun X, Dang L, Li D, Qi J, Wang M et al. Structural basis of Glycan recognition in globally predominant human P[8] rotavirus. Virol Sin 2020; 35:156–170 [View Article][PubMed]
    [Google Scholar]
  22. Zeller M, Heylen E, De Coster S, Van Ranst M, Matthijnssens J. Full genome characterization of a porcine-like human G9P[6] rotavirus strain isolated from an infant in Belgium. Infect Genet Evol 2012; 12:1492–1500 [View Article][PubMed]
    [Google Scholar]
  23. Doan YH, Nakagomi T, Aboudy Y, Silberstein I, Behar-Novat E et al. Identification by full-genome analysis of a bovine rotavirus transmitted directly to and causing diarrhea in a human child. J Clin Microbiol 2013; 51:182–189 [View Article][PubMed]
    [Google Scholar]
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