1887

Abstract

In Japan, tulip-growing areas have been plagued by viral diseases for decades, but the viruses causing the damage remain undescribed. In this study, and plants mechanically inoculated with crude sap from a symptomatic tulip flower exhibited necrosis symptoms. Additionally, flexuous and filamentous virus particles were detected by electron microscopy analysis. Moreover, we determined the complete sequences of two genomic segments of the tulip streak virus (TuSV), which is a new virus associated with streaking symptoms, on the basis of a next-generation sequencing analysis. Homology analyses of the amino acid sequence of RNA-dependent RNA polymerase and the terminal sequence of the genomic RNA indicated that TuSV is associated with viruses in the family , but differs substantially from other reported viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001525
2020-11-23
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/102/2/vir001525.html?itemId=/content/journal/jgv/10.1099/jgv.0.001525&mimeType=html&fmt=ahah

References

  1. Niisato Y, Niisato Y, Niisato Y, Niisato Y. Dynamics of production and trade of flower bulbs in the Netherlands, China and Japan. https://core.ac.uk/download/pdf/160745818.pdf. 2018. [accessed 20 Aug 2020].
  2. Ministry of Agriculture, Forestry and Fisheries 2019; Statistics on flowering plants. https://www.maff.go.jp/j/seisan/kaki/flower/f-kaboku.html 11 Jun 2020
  3. McGovern RJ, Elmer WH. Diseases of Tulip. In: Handbook of Florists' Crops Diseases. Handbook of Plant Disease Management Cham: Springer,; 2017
    [Google Scholar]
  4. Morikawa T, Nomura Y, Yamamoto T, Natsuaki T. Partial characterization of virus-like particles associated with tulip mild mottle mosaic. Jpn J Phytopathol 1995; 61:578–581 [View Article]
    [Google Scholar]
  5. Morikawa T, Ohoura K, Yamamoto T, Nomura Y, Matsumoto M et al. Occurrence of virus-like diseases of tulip, mild mottle mosaic and streaking, in Toyama prefecture. Bull Toyama Agric Res Ctr 1995; 16:55–66
    [Google Scholar]
  6. García ML, BóE D, da Graça JV, Gago-Zachert S, Hammond J et al. Proposal 2017.002P.A.v2.Ophioviridae_ren. Change the name of the family Ophioviridae to Aspiviridae and adopt nonlatinised binomial names for its constituent species. http://ictv.global/proposals-17/2017.002P.A.v2.Ophioviridae_ren.zip [accessed 25 Apr 2018] .
  7. Morikawa T, Chikuo Y, Natsuaki T. Transmission of tulip mild mottle mosaic by Olpidium brassicae . Jpn J Phytopathol 1997; 63:504
    [Google Scholar]
  8. Morikawa T, Horii K, Mochizuki T, Ohki T, Tsuda S et al. Transmissibility with Tulip streak virus and Tulip mild mottle mosaic virus, and host specificity of Olpidium spp. Proc. Assoc. Plant Prot Hokuriku 2007; 56:37–40
    [Google Scholar]
  9. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for illumina sequence data. Bioinformatics 2014; 30:2114–2120 [View Article][PubMed]
    [Google Scholar]
  10. Nurk S, Meleshko D, Korobeynikov A, Pevzner PA. metaSPAdes: a new versatile metagenomic assembler. Genome Res 2017; 27:824–834 [View Article][PubMed]
    [Google Scholar]
  11. Mielke N, Muehlbach H-P. A novel, multipartite, negative-strand RNA virus is associated with the ringspot disease of European mountain ash (Sorbus aucuparia L.). J Gen Virol 2007; 88:1337–1346 [View Article][PubMed]
    [Google Scholar]
  12. Elliott RM, Brennan B. Emerging phleboviruses. Curr Opin Virol 2014; 5:50–57 [View Article][PubMed]
    [Google Scholar]
  13. Navarro B, Minutolo M, De Stradis A, Palmisano F, Alioto D et al. The first phlebo-like virus infecting plants: a case study on the adaptation of negative-stranded RNA viruses to new hosts. Mol Plant Pathol 2018; 19:1075–1089 [View Article][PubMed]
    [Google Scholar]
  14. Falk BW, Tsai JH. Biology and molecular biology of viruses in the genus Tenuivirus . Annu Rev Phytopathol 1998; 36:139–163 [View Article][PubMed]
    [Google Scholar]
  15. Li C-X, Shi M, Tian J-H, Lin X-D, Kang Y-J et al. Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. elife 2015; 4:e05378 [View Article][PubMed]
    [Google Scholar]
  16. Wright AA, Szostek SA, Beaver-Kanuya E, Harper SJ. Diversity of three bunya-like viruses infecting apple. Arch Virol 2018; 163:3339–3343 [View Article][PubMed]
    [Google Scholar]
  17. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Evol Genet Anal 2016; 33:1870–1874
    [Google Scholar]
  18. Liu D-Y, Tesh RB, Travassos da Rosa APA, Peters CJ, Yang Z et al. Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses. J Gen Virol 2003; 84:465–473 [View Article][PubMed]
    [Google Scholar]
  19. International Committee on Taxonomy of Viruses ICTV master species list 2019.v1. https://talk.ictvonline.org/files/master-species-lists/m/msl/9601 29 Apr 2020
  20. Vaira AM, Miozzi L, Vallino M, Carra A, Lenzi R et al. Evidence of new viruses infecting freesia hybrids showing necrotic disease. Acta Hortic 2018; 1193:21–28 [View Article]
    [Google Scholar]
  21. Xin M, Cao M, Liu W, Ren Y, Zhou X et al. Two negative-strand RNA viruses identified in watermelon represent a novel clade in the order Bunyavirales. Front Microbiol 2017; 8:1514 [View Article][PubMed]
    [Google Scholar]
  22. Csorba T, Kontra L, Burgyán J. viral silencing suppressors: Tools forged to fine-tune host-pathogen coexistence. Virology 2015; 479-480:85–103 [View Article][PubMed]
    [Google Scholar]
  23. Gupta AK, Hein GL, Graybosch RA, Tatineni S. Octapartite negative-sense RNA genome of High Plains wheat mosaic virus encodes two suppressors of RNA silencing. Virology 2018; 518:152–162 [View Article][PubMed]
    [Google Scholar]
  24. Leastro MO, Pallás V, Resende RO, Sánchez-Navarro JA. The movement proteins (NSm) of distinct tospoviruses peripherally associate with cellular membranes and interact with homologous and heterologous NSm and nucleocapsid proteins. Virology 2015; 478:39–49 [View Article][PubMed]
    [Google Scholar]
  25. Ishikawa K, Maejima K, Komatsu K, Netsu O, Keima T et al. Fig mosaic emaravirus p4 protein is involved in cell-to-cell movement. J Gen Virol 2013; 94:682–686 [View Article][PubMed]
    [Google Scholar]
  26. Yu C, Karlin DG, Lu Y, Wright K, Chen J et al. Experimental and bioinformatic evidence that raspberry leaf blotch emaravirus P4 is a movement protein of the 30K superfamily. J Gen Virol 2013; 94:2117–2128 [View Article][PubMed]
    [Google Scholar]
  27. Nishiguchi M, Kobayashi K. Attenuated plant viruses: preventing virus diseases and understanding the molecular mechanism. J Gen Plant Pathol 2011; 77:221–229 [View Article]
    [Google Scholar]
  28. Starr EP, Nuccio EE, Pett-Ridge J, Banfield JF, Firestone MK. Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil. Proc Natl Acad Sci U S A 2019; 116:25900–25908 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001525
Loading
/content/journal/jgv/10.1099/jgv.0.001525
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error