1887

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Volume 102, Issue 2

Identification of miRNAs encoded by Autographa californica nucleopolyhedrovirus Free

Abstract

Two Autographa californica nucleopolyhedrovirus (AcMNPV) encoded miRNAs, AcMNPV-miR-1 and AcMNPV-miR-3, have been reported by us in 2013 and 2019, respectively. Here, we present an integrated investigation of AcMNPV-encoded miRNAs, which include the above two miRNAs and three additional newly identified miRNAs. Six candidate miRNAs were predicted through small RNA deep sequencing and bioinformatics, of which, five were validated. Three miRNAs are located opposite the coding sequences, the other two are located in the coding sequences of viral genes. Targets in both virus and host were predicted and subsequently tested using dual-luciferase reporter assays. The validated targets were found mainly in AcMNPV, except for the targets of AcMNPV-miR-4, which are all host genes. Based on reporter assays, the five miRNAs predominantly function by down-regulating their targets. The transcription start sites of these miRNAs were bioinformatic screened based on known baculovirus promoter motifs. Our study reveals that AcMNPV-encoded miRNAs function as fine modulators of the interactions between host and virus by regulating viral and/or host genes.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Baculovirus; AcMNPV , Host , miRNA , Regulation and Target
Funding
This study was supported by the:
  • Natural Science Foundation of Guangdong Province (Award 2015A030313100)
    • Principle Award Recipient: Jinwen Wang
  • National Natural Science Foundation of China (Award 30970139)
    • Principle Award Recipient: Xunzhang Wang
  • National Natural Science Foundation of China (Award 31872024)
    • Principle Award Recipient: Jinwen Wang
© 2021 The Authors
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/content/journal/jgv/10.1099/jgv.0.001510
2020-11-25
2024-04-18
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Identification of miRNAs encoded by Autographa californica nucleopolyhedrovirus
J. Gen. Virol. 102, 001510 (2021); https://doi.org/10.1099/jgv.0.001510
/content/journal/jgv/10.1099/jgv.0.001510
/content/journal/jgv/10.1099/jgv.0.001510
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