1887

Abstract

Human respiratory syncytial virus (HRSV) is an important respiratory pathogen causing a spectrum of illness, from common cold-like symptoms, to bronchiolitis and pneumonia requiring hospitalization in infants, the immunocompromised and the elderly. HRSV exists as two antigenic subtypes, A and B, which typically cycle biannually in separate seasons. There are many unresolved questions in HRSV biology regarding the interactions and interplay of the two subtypes. Therefore, we generated a reverse genetics system for a subtype A HRSV from the 2011 season (A11) to complement our existing subtype B reverse genetics system. We obtained the sequence (HRSV) directly from an unpassaged clinical sample and generated the recombinant (r) HRSV. A version of the virus expressing enhanced green fluorescent protein (EGFP) from an additional transcription unit in the fifth (5) position of the genome, rHRSVEGFP(5), was also generated. rHRSV and rHRSVEGFP(5) grew comparably in cell culture. To facilitate animal co-infection studies, we derivatized our subtype B clinical isolate using reverse genetics toexpress the red fluorescent protein (dTom)-expressing rHRSVdTom(5). These viruses were then used to study simultaneous co-infection of the respiratory tract. Following intranasal infection, both rHRSVEGFP(5) and rHRSVdTom(5) infected cotton rats targeting the same cell populations and demonstrating that co-infection occurs . The implications of this finding on viral evolution are important since it shows that inter-subtype cooperativity and/or competition is feasible during the natural course of the infection.

Funding
This study was supported by the:
  • Medical Research Council (Award G0801001)
    • Principle Award Recipient: Paul Duprex
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2020-07-29
2021-08-02
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