1887

Abstract

Emerging coronaviruses represent serious threats to human and animal health worldwide, and no approved therapeutics are currently available. Here, we used Transmissible gastroenteritis virus (TGEV) as the alpha-coronavirus model, and investigated the antiviral properties of curcumin against TGEV. Our results demonstrated that curcumin strongly inhibited TGEV proliferation and viral protein expression in a dose-dependent manner. We also observed that curcumin exhibited direct virucidal abilities in a dose-, temperature- and time-dependent manner. Furthermore, time-of-addition assays showed that curcumin mainly acted in the early phase of TGEV replication. Notably, in an adsorption assay, curcumin at 40 µM resulted in a reduction in viral titres of 3.55 log TCID ml, indicating that curcumin possesses excellent inhibitory effects on the adsorption of TGEV. Collectively, we demonstrate for the first time that curcumin has virucidal activity and virtual inhibition against TGEV, suggesting that curcumin might be a candidate drug for effective control of TGEV infection.

Funding
This study was supported by the:
  • Hubei Provincial Department of Education (Award B2016303)
    • Principle Award Recipient: Lilan Xie
  • Natural Science Foundation of Hubei Province (Award 2018CFB662)
    • Principle Award Recipient: Lilan Xie
  • National Natural Science Foundation of China (Award 31402181)
    • Principle Award Recipient: Lilan Xie
  • National Natural Science Foundation of China (Award 31972692)
    • Principle Award Recipient: Lilan Xie
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2020-07-17
2024-10-11
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References

  1. Perlman S. Research driven by curiosity: the journey from basic molecular biology and virology to studies of human pathogenic coronaviruses. PLoS Pathog 2015; 11:e1005023 [View Article][PubMed]
    [Google Scholar]
  2. Payne S. Chapter 17 - Family Coronaviridae Viruses, Academic Press; 2017 pp 149–158
    [Google Scholar]
  3. Brian DA, Baric RS. Coronavirus genome structure and replication. Curr Top Microbiol Immunol 2005; 287:1–30 [View Article][PubMed]
    [Google Scholar]
  4. Mullan BP, Davies GT, Cutler RS. Simulation of the economic impact of transmissible gastroenteritis on commercial pig production in Australia. Aust Vet J 1994; 71:151–154 [View Article][PubMed]
    [Google Scholar]
  5. Xia L, Yang Y, Wang J, Jing Y, Yang Q. Impact of TGEV infection on the pig small intestine. Virol J 2018; 15:102 [View Article][PubMed]
    [Google Scholar]
  6. Ren X, Meng F, Yin J, Li G, Li X et al. Action mechanisms of lithium chloride on cell infection by transmissible gastroenteritis coronavirus. PLoS One 2011; 6:e18669 [View Article][PubMed]
    [Google Scholar]
  7. Zou H, Zarlenga DS, Sestak K, Suo S, Ren X. Transmissible gastroenteritis virus: identification of M protein-binding peptide ligands with antiviral and diagnostic potential. Antiviral Res 2013; 99:383–390 [View Article][PubMed]
    [Google Scholar]
  8. Moghadamtousi SZ, Kadir HA, Hassandarvish P, Tajik H, Abubakar S et al. A review on antibacterial, antiviral, and antifungal activity of curcumin. Biomed Res Int 2014; 2014:186864 [View Article][PubMed]
    [Google Scholar]
  9. Praditya D, Kirchhoff L, Brüning J, Rachmawati H, Steinmann J et al. Anti-infective properties of the golden spice curcumin. Front Microbiol 2019; 10:912 [View Article][PubMed]
    [Google Scholar]
  10. Balasubramanian A, Pilankatta R, Teramoto T, Sajith AM, Nwulia E et al. Inhibition of dengue virus by curcuminoids. Antiviral Res 2019; 162:71–78 [View Article][PubMed]
    [Google Scholar]
  11. Hesari A, Ghasemi F, Salarinia R, Biglari H, Tabar Molla Hassan A et al. Effects of curcumin on NF-κB, AP-1, and Wnt/β-catenin signaling pathway in hepatitis B virus infection. J Cell Biochem 2018; 119:7898–7904 [View Article][PubMed]
    [Google Scholar]
  12. Mounce BC, Cesaro T, Carrau L, Vallet T, Vignuzzi M. Curcumin inhibits Zika and Chikungunya virus infection by inhibiting cell binding. Antiviral Res 2017; 142:148–157 [View Article][PubMed]
    [Google Scholar]
  13. Zouharova D, Lipenska I, Fojtikova M, Kulich P, Neca J et al. Antiviral activities of 2,6-diaminopurine-based acyclic nucleoside phosphonates against herpesviruses: in vitro study results with pseudorabies virus (PrV, SuHV-1). Vet Microbiol 2016; 184:84–93 [View Article][PubMed]
    [Google Scholar]
  14. Wei Z, Burwinkel M, Palissa C, Ephraim E, Schmidt MFG. Antiviral activity of zinc salts against transmissible gastroenteritis virus in vitro. Vet Microbiol 2012; 160:468–472 [View Article][PubMed]
    [Google Scholar]
  15. Zhou Y, Wu W, Xie L, Wang D, Ke Q et al. Cellular RNA helicase DDX1 is involved in transmissible gastroenteritis virus nsp14-Induced interferon-beta production. Front Immunol 2017; 8:940 [View Article][PubMed]
    [Google Scholar]
  16. Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin: a short review. Life Sci 2006; 78:2081–2087 [View Article][PubMed]
    [Google Scholar]
  17. Colpitts CC, Schang LM, Rachmawati H, Frentzen A, Pfaender S et al. Turmeric curcumin inhibits entry of all hepatitis C virus genotypes into human liver cells. Gut 2014; 63:1137–1149 [View Article][PubMed]
    [Google Scholar]
  18. Xu W, Li Q. Progress in the development of aminopeptidase N (APN/CD13) inhibitors. Curr Med Chem Anticancer Agents 2005; 5:281–301 [View Article][PubMed]
    [Google Scholar]
  19. Yang J, Zhu D, Liu S, Shao M, Liu Y et al. Curcumin enhances radiosensitization of nasopharyngeal carcinoma by regulating circRNA network. Mol Carcinog 2020; 59:202–214 [View Article][PubMed]
    [Google Scholar]
  20. An K, Fang L, Luo R, Wang D, Xie L et al. Quantitative proteomic analysis reveals that transmissible gastroenteritis virus activates the Jak-STAT1 signaling pathway. J Proteome Res 2014; 13:5376–5390 [View Article][PubMed]
    [Google Scholar]
  21. Ali A, Banerjea AC. Curcumin inhibits HIV-1 by promoting Tat protein degradation. Sci Rep 2016; 6:27539 [View Article][PubMed]
    [Google Scholar]
  22. Dai J, Gu L, Su Y, Wang Q, Zhao Y et al. Inhibition of curcumin on influenza A virus infection and influenzal pneumonia via oxidative stress, TLR2/4, p38/JNK MAPK and NF-κB pathways. Int Immunopharmacol 2018; 54:177–187 [View Article][PubMed]
    [Google Scholar]
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