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Volume 101, Issue 7

Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses Open Access

Abstract

In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expressing NA N1 gene of AIV A/Chicken/Malaysia/5858/2004, and chicken IL-12 gene. Our data shows that the N1 and IL-12 proteins were successfully expressed from the recombinants with 48 kD and 70 kD molecular weights, respectively. Upon inoculation into specific-pathogen-free (SPF) chickens at 10 p.f.u. ml, levels of CD3+/CD4+ and CD3+/CD8+ populations were higher in the wild-type fowlpox virus FP9 strain, compared to those of rFWPV-N1 and rFWPV-N1-IL-12 at weeks 2 and 5 time points. Furthermore, rFWPV-N1-IL-12 showed a suppressive effect on chicken body weight within 4 weeks after inoculation. We suggest that co-expression of N1 with or without IL-12 offers undesirable quality as a potential AIV vaccine candidate.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): avian influenza virus , cytokine , IL-12 , neuraminidase and recombinant fowlpox virus
Funding
This study was supported by the:
  • Ministry of Higher Education Malaysia (Institute of Bioscience’s Higher Institution Centre of Excellence (IBS HICoE)) (Award 6369101)
    • Principle Award Recipient: Abdul Rahman Omar
  • Ministry of Higher Education Malaysia (Universiti Putra Malaysia's Geran Putra) (Award 9623200)
    • Principle Award Recipient: Abdul Razak Mariatulqabtiah
  • Universiti Putra Malaysia's Graduate Research Fellowship (GRF) (Award -)
    • Principle Award Recipient: Nadzreeq Nor Majid
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2020-05-19
2024-04-23
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Negligible effect of chicken cytokine IL-12 integration into recombinant fowlpox viruses expressing avian influenza virus neuraminidase N1 on host cellular immune responses
J. Gen. Virol. 101, 772 (2020); https://doi.org/10.1099/jgv.0.001428
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