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Abstract

In comparison to the extensive characterization of haemagglutinin antibodies of avian influenza virus (AIV), the role of neuraminidase (NA) as an immunogen is less well understood. This study describes the construction and cellular responses of recombinant fowlpox viruses (rFWPV) strain FP9, co-expressing NA N1 gene of AIV A/Chicken/Malaysia/5858/2004, and chicken IL-12 gene. Our data shows that the N1 and IL-12 proteins were successfully expressed from the recombinants with 48 kD and 70 kD molecular weights, respectively. Upon inoculation into specific-pathogen-free (SPF) chickens at 10 p.f.u. ml, levels of CD3+/CD4+ and CD3+/CD8+ populations were higher in the wild-type fowlpox virus FP9 strain, compared to those of rFWPV-N1 and rFWPV-N1-IL-12 at weeks 2 and 5 time points. Furthermore, rFWPV-N1-IL-12 showed a suppressive effect on chicken body weight within 4 weeks after inoculation. We suggest that co-expression of N1 with or without IL-12 offers undesirable quality as a potential AIV vaccine candidate.

Funding
This study was supported by the:
  • Abdul Rahman Omar , Ministry of Higher Education Malaysia (Institute of Bioscience’s Higher Institution Centre of Excellence (IBS HICoE)) , (Award 6369101)
  • Abdul Razak Mariatulqabtiah , Ministry of Higher Education Malaysia (Universiti Putra Malaysia's Geran Putra) , (Award 9623200)
  • Nadzreeq Nor Majid , Universiti Putra Malaysia's Graduate Research Fellowship (GRF) , (Award -)
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/content/journal/jgv/10.1099/jgv.0.001428
2020-05-19
2020-06-04
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