1887

Abstract

Yak is an iconic species of the Qinghai–Tibet Plateau, which is the world's highest plateau. Here, a total of 541 yak diarrhoeic samples were collected from 69 farms in four provinces in the Qinghai–Tibet Plateau from April 2015 to June 2018, and 73.6 % of samples were detected as Bovine Rotavirus A (BRVA) positive by RT-PCR assay. Two G genotypes (G6, G10) and two P genotypes (P[1], P[11]) were determined, in which G6P[1] BRVA was the predominant strain. Moreover, VP7 and VP4 of these G6P[1] strains showed unique amino acid mutations, such that they clustered into an independent branch in the phylogenetic tree. A strain of BRVA designated as RVA/Yak-tc/CHN/QH-1/2015/G6P[1] was isolated successfully using MA104 cells, and the virus titre was determined as 10 TCID ml. The genome of strain QH-1 had a G6-P[1]-I2-R2-C2-M2-A3-N3-T6-E2-H3 genotype constellation. QH-1 was identified as a reassortment strain of BRVA, human RVA and ovine RVA based on the nucleotide identity and phylogenetic tree of 11 gene segments, indicating its public health significance. To the best of our knowledge, this is the first report on the molecular prevalence and genome characteristics of BRVA in yak, contributing to further understanding of the epidemic and genetic evolution of BRVA.

Keyword(s): prevalence , genome , BRVA , G6P[1] and yak
Funding
This study was supported by the:
  • Nan Yan , The Innovation Team of Beef Cattle, Sichuan Province
  • Nan Yan , The 13th Five-Year Plan National Science and Technology Support Program , (Award 2016YFD0500907)
  • Not Applicable , The innovation team for emerging animal diseases on Qinghai-Tibet Plateau, Southwest Minzu University , (Award 2020NTD02)
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/content/journal/jgv/10.1099/jgv.0.001426
2020-05-19
2020-06-04
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