1887

Journal of General Virology

Volume 101, Issue 5

Parainfluenza virus 5 fusion protein maintains pre-fusion stability but not fusogenic activity following mutation of a transmembrane leucine/isoleucine domain Free

Abstract

The paramyxoviruses Hendra virus (HeV) and parainfluenza virus 5 (PIV5) require the fusion (F) protein to efficiently infect cells. For fusion to occur, F undergoes dramatic, essentially irreversible conformational changes to merge the viral and cell membranes into a continuous bilayer. Recently, a transmembrane (TM) domain leucine/isoleucine (L/I) zipper was shown to be critical in maintaining the expression, stability and pre-fusion conformation of HeV F, allowing for fine-tuned timing of membrane fusion. To analyse the effect of the TM domain L/I zipper in another paramyxovirus, we created alanine mutations to the TM domain of PIV5 F, a paramyxovirus model system. Our data show that while the PIV5 F TM L/I zipper does not significantly affect total expression and only modestly affects surface expression and pre-fusion stability, it is critical for fusogenic activity. These results suggest that the roles of TM L/I zipper motifs differ among members of the family .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): fusogenic activity , leucine/isoleucine zipper , pre-fusion stability and transmembrane domain
Funding
This study was supported by the:
  • National Institute of General Medical Sciences (Award 2P20 RR02017)
    • Principle Award Recipient: Rebecca Ellis Dutch
  • National Institute of Allergy and Infectious Diseases (Award R01AI051517)
    • Principle Award Recipient: Rebecca Ellis Dutch
© 2020 The Authors
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2020-02-25
2021-07-26
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Parainfluenza virus 5 fusion protein maintains pre-fusion stability but not fusogenic activity following mutation of a transmembrane leucine/isoleucine domain
J. Gen. Virol. 101, 467 (2020); https://doi.org/10.1099/jgv.0.001399
/content/journal/jgv/10.1099/jgv.0.001399
/content/journal/jgv/10.1099/jgv.0.001399
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