RT Journal Article SR Electronic(1) A1 Han, Kelei A1 Zheng, Hongying A1 Ji, Mengfei A1 Cui, Weijun A1 Hu, Shuzhen A1 Peng, Jiejun A1 Zhao, Jinping A1 Lu, Yuwen A1 Lin, Lin A1 Liu, Yong A1 Chen, Jianping A1 Yan, FeiYR 2020 T1 A single amino acid in coat protein of Pepper mild mottle virus determines its subcellular localization and the chlorosis symptom on leaves of pepper JF Journal of General Virology, VO 101 IS 5 SP 565 OP 570 DO https://doi.org/10.1099/jgv.0.001398 PB Microbiology Society, SN 1465-2099, AB Pepper mild mottle virus (PMMoV) causes serious economic losses in pepper production in China. In a survey for viral diseases on pepper, two PMMoV isolates (named PMMoV-ZJ1 and PMMoV-ZJ2) were identified with different symptoms in Zhejiang province. Sequence alignment analysis suggested there were only four amino acid differences between the isolates: Val262Gly, Ile629Met and Ala1164Thr in the replicase, and Asp20Asn in the coat protein. Infectious cDNA clones of both isolates were constructed and shown to cause distinctive symptoms. Chlorosis symptoms appeared only on PMMoV-ZJ2-infected plants and the Asp20Asn substitution in the CP was shown to be responsible. Confocal assays revealed that the subcellular localization pattern of the two CPs was different, CP20Asp was mainly located at the cell periphery, whereas most CP20Asn located in the chloroplast. Thus, a single amino acid in the CP determined the chlorosis symptom, accompanied by an altered subcellular localization., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001398