1887

Journal of General Virology

Diaphorina citri densovirus is a persistently infecting virus with a hybrid genome organization and unique transcription strategy No Access

Abstract

Diaphorina citri densovirus (DcDV) is an ambisense densovirus with a 5071 nt genome. Phylogenetic analysis places DcDV in an intermediate position between those in the and genera, a finding that is consistent with the observation that DcDV possesses an -like non-structural (NS) protein-gene cassette, but a capsid-protein (VP) gene cassette resembling those of other ambisense densoviruses. DcDV is maternally transmitted to 100 % of the progeny of infected female , and the progeny of infected females carry DcDV as a persistent infection without outward phenotypic effects. We were unable to infect naïve individuals by oral inoculation, however low levels of transient viral replication are detected following intrathoracic injection of DcDV virions into uninfected insects. Transcript mapping indicates that DcDV produces one transcript each from the NS and VP gene cassettes and that these transcripts are polyadenylated at internal sites to produce a ~2.2 kb transcript encoding the NS proteins and a ~2.4 kb transcript encoding the VP proteins. Additionally, we found that transcriptional readthrough leads to the production of longer non-canonical transcripts from both genomic strands.

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Keyword(s): Asian Citrus Psyllid , densovirus , parvovirus and Diaphorina citri
© 2019 The Authors
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/content/journal/jgv/10.1099/jgv.0.001371
2019-12-19
2020-01-24
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Diaphorina citri densovirus is a persistently infecting virus with a hybrid genome organization and unique transcription strategy
Microbiology Society , jgv001371; https://doi.org/10.1099/jgv.0.001371
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