@article{mbs:/content/journal/jgv/10.1099/jgv.0.001359, author = "Simari, Milagros Belén and Goñi, Sandra Elizabeth and Luppo, Victoria Celina and Fabbri, Cintia Marcela and Argüelles, Marcelo Horacio and Lozano, Mario Enrique and Morales, Maria Alejandra and Iglesias, Néstor Gabriel", title = "Specific diagnostic method for St. Louis encephalitis virus using a non-structural protein as the antigen", journal= "Journal of General Virology", year = "2020", volume = "101", number = "2", pages = "168-174", doi = "https://doi.org/10.1099/jgv.0.001359", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001359", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "Flavivirus", keywords = "SLEV diagnosis", keywords = "NS5", abstract = "St. Louis encephalitis virus (SLEV) is a mosquito-borne re-emerging flavivirus in Argentina. It is currently necessary to develop specific serological tests that can efficiently discriminate the flaviviruses that circulate in our country. The immunoassays to diagnose SLEV lack specificity because they are based on the detection of structural viral proteins and the human immunoglobulins produced during infection against these proteins cross-react with other flaviviruses. Here, we describe an enzyme-immunoassay designed to detect human IgG antibodies specific to the viral non-structural protein NS5. The results indicate that NS5 is a promising antigen useful to discriminate SLEV from other circulating flaviviruses.", }