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Abstract

Triniti virus (TNTV) has been isolated in Trinidad and Tobago and in Brazil. To date little is known about this virus, which is classified as an ungrouped virus within the family Togaviridae. Here, three isolates of TNTV were characterized both genetically and antigenically. The genome was shown to contain three RNA segments: small (S), medium (M) and large (L). Genome organization, protein sizes and protein motifs were similar to those of viruses in the genus Orthobunyavirus, family Peribunyaviridae. Antigenic reactivity revealed the three TNTV isolates to be closely related, but no serologic cross-reaction with other orthobunyaviruses. Morphological observation by transmission electron microscopy indicated that virus size and symmetry were compatible with those of viruses in the family Peribunyaviridae. Our serological, morphological and molecular results support the taxonomic reclassification of TNTV as a member of the genus Orthobunyavirus, family Peribunyaviridae.

  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2018-12-14
2024-12-08
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References

  1. Karabatsos N. International Catalogue of Arboviruses, including certain other viruses of vertebrates; 1985
  2. Spence L, Anderson CR, Aitken TH, Downs WG. Triniti virus, a new agent isolated from trinidadian mosquitoes. Am J Trop Med Hyg 1964; 13:114–117 [View Article][PubMed]
    [Google Scholar]
  3. El Mekki AA, Nieuwenhuysen P, van der Groen G, Pattyn SR. Characterization of some ungrouped viruses. Trans R Soc Trop Med Hyg 1981; 75:799–806 [View Article][PubMed]
    [Google Scholar]
  4. Calisher CH, Shope RE, Brandt W, Casals J, Karabatsos N et al. Proposed antigenic classification of registered arboviruses I. Togaviridae, Alphavirus. Intervirology 1980; 14:229–232 [View Article][PubMed]
    [Google Scholar]
  5. Lefkowitz EJ, Dempsey DM, Hendrickson RC, Orton RJ, Siddell SG et al. Virus taxonomy: the database of the International Committee on Taxonomy of Viruses (ICTV). Nucleic Acids Research 1977; 2017:1–10
    [Google Scholar]
  6. Hervé JP, Dégallier N, Travassos da Rosa APA, Pinheiro Fp SFG. Arboviroses - aspectos ecológicos - instituto evandro chagas: 50 anos de contribuição às ciências biológicas e à medicina tropical; 1986409–437
  7. Travassos da Rosa JFS, Travassos da Rosa APA, Vasconcelos, Pinheiro, Rodrigues et al. Arboviruses isolated in the Evandro Chagas Institute, including some described for the first time in the Brazilian Amazon region, their known hosts, and their pathology for man. An Overview of Arbovirology in Brazil and Neighbouring Countries Belém: Instituto Evandro Chagas; 1998 pp. 19–31
    [Google Scholar]
  8. Fulton F, Dumbell KR. The serological comparison of strains of influenza virus. J Gen Microbiol 1949; 3:97–111 [View Article][PubMed]
    [Google Scholar]
  9. Rothberg JM, Hinz W, Rearick TM, Schultz J, Mileski W et al. An integrated semiconductor device enabling non-optical genome sequencing. Nature 2011; 475:348–352 [View Article][PubMed]
    [Google Scholar]
  10. Sanger F, Coulson AR. A rapid method for determining sequences in DNA by primed synthesis with DNA polymerase. J Mol Biol 1975; 94:441–448 [View Article][PubMed]
    [Google Scholar]
  11. Chevreux B, Wetter T, Suhai S. Genome sequence assembly using trace signals and additional sequence information. computer science and biology . Proceedings of the German Conference on Bioinformatics 199945–56
    [Google Scholar]
  12. Pei J, Grishin N V, Road FP. Multiple Sequence Alignment Methods Totowa, NJ: Humana Press; 2014
    [Google Scholar]
  13. Myung IJ. Tutorial on maximum likelihood estimation. J Math Psychol 2003; 47:90–100 [View Article]
    [Google Scholar]
  14. Stamatakis A. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article][PubMed]
    [Google Scholar]
  15. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  16. Katoh K, Misawa K, Kuma K, Miyata T. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 2002; 30:3059–3066 [View Article][PubMed]
    [Google Scholar]
  17. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS et al. Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 1999; 73:152–160[PubMed]
    [Google Scholar]
  18. Müller R, Poch O, Delarue M, Bishop DH, Bouloy M et al. Rift Valley fever virus L segment: correction of the sequence and possible functional role of newly identified regions conserved in RNA-dependent polymerases. J Gen Virol 1994; 75:1345–1352 [View Article][PubMed]
    [Google Scholar]
  19. Dixon LK, Alonso C, Escribano JM, Martins JM, Revilla Y et al. ICTV virus taxonomy 2012. ICTV Commitee 2012153–162
    [Google Scholar]
  20. Maes P, Alkhovsky SV, Bào Y, Beer M, Birkhead M et al. Taxonomy of the family Arenaviridae and the order Bunyavirales: update 2018. Arch Virol 2018; 163:2295–2310 [View Article][PubMed]
    [Google Scholar]
  21. Bridgen A, Weber F, Fazakerley JK, Elliott RM. Bunyamwera bunyavirus nonstructural protein NSs is a nonessential gene product that contributes to viral pathogenesis. Proc Natl Acad Sci USA 2001; 98:664–669 [View Article][PubMed]
    [Google Scholar]
  22. Lambert AJ, Lanciotti RS. Molecular characterization of medically important viruses of the genus Orthobunyavirus. J Gen Virol 2008; 89:2580–2585 [View Article][PubMed]
    [Google Scholar]
  23. Pachler K, Růžek D, Nowotny N. Molecular characterization of the African orthobunyavirus Ilesha virus. Infect Genet Evol 2013; 20:124–130 [View Article][PubMed]
    [Google Scholar]
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