1887

Abstract

The spike glycoprotein (S) of infectious bronchitis virus (IBV) comprises two subunits, S1 and S2. We have previously demonstrated that the S2 subunit of the avirulent Beau-R strain is responsible for its extended cellular tropism for Vero cells. Two recombinant infectious bronchitis viruses (rIBVs) have been generated; the immunogenic S1 subunit is derived from the IBV vaccine strain, H120, or the virulent field strain, QX, within the genetic background of Beau-R. The rIBVs BeauR-H120(S1) and BeauR-QX(S1) are capable of replicating in primary chicken kidney cell cultures and in Vero cells. These results demonstrate that rIBVs are able to express S1 subunits from genetically diverse strains of IBV, which will enable the rational design of a future generation of IBV vaccines that may be grown in Vero cells.

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2018-10-24
2020-01-24
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References

  1. Cavanagh D, Naqi S. Infectious bronchitis. In Saif YM, Barnes HJ, Glisson JR, Fadly AM, McDougald LR et al. (editors) Diseases of Poultry, 11th ed. Ames, Iowa: Iowa State University Press; 2003; pp.101–119
    [Google Scholar]
  2. Britton P, Cavanagh D. Avian coronavirus diseases and infectious bronchitis vaccine development. In Thiel V. (editor) Coronaviruses: Molecular and Cellular Biology Norfolk, UK: Caister Academic Press; 2007; pp.161–181
    [Google Scholar]
  3. Hodgson T, Casais R, Dove B, Britton P, Cavanagh D. Recombinant infectious bronchitis coronavirus Beaudette with the spike protein gene of the pathogenic M41 strain remains attenuated but induces protective immunity. J Virol 2004;78:13804–13811 [CrossRef][PubMed]
    [Google Scholar]
  4. Ambali AG, Jones RC. Early pathogenesis in chicks of infection with an enterotropic strain of infectious bronchitis virus. Avian Dis 1990;34:809–817 [CrossRef][PubMed]
    [Google Scholar]
  5. Cavanagh D. Coronavirus avian infectious bronchitis virus. Vet Res 2007;38:281–297 [CrossRef][PubMed]
    [Google Scholar]
  6. Lambrechts C, Pensaert M, Ducatelle R. Challenge experiments to evaluate cross-protection induced at the trachea and kidney level by vaccine strains and Belgian nephropathogenic isolates of avian infectious bronchitis virus. Avian Pathol 1993;22:577–590 [CrossRef][PubMed]
    [Google Scholar]
  7. Ziegler AF, Ladman BS, Dunn PA, Schneider A, Davison S et al. Nephropathogenic infectious bronchitis in Pennsylvania chickens 1997–2000. Avian Dis 2002;46:847–858 [CrossRef][PubMed]
    [Google Scholar]
  8. Sánchez CM, Izeta A, Sánchez-Morgado JM, Alonso S, Sola I et al. Targeted recombination demonstrates that the spike gene of transmissible gastroenteritis coronavirus is a determinant of its enteric tropism and virulence. J Virol 1999;73:7607–7618[PubMed]
    [Google Scholar]
  9. Tekes G, Hofmann-Lehmann R, Bank-Wolf B, Maier R, Thiel HJ et al. Chimeric feline coronaviruses that encode type II spike protein on type I genetic background display accelerated viral growth and altered receptor usage. J Virol 2010;84:1326–1333 [CrossRef][PubMed]
    [Google Scholar]
  10. Cunningham CH, Spring MP, Nazerian K. Replication of avian infectious bronchitis virus in African green monkey kidney cell line VERO. J Gen Virol 1972;16:423–427 [CrossRef][PubMed]
    [Google Scholar]
  11. Otsuki K, Noro K, Yamamoto H, Tsubokura M. Studies on avian infectious bronchitis virus (IBV). II. Propagation of IBV in several cultured cells. Arch Virol 1979;60:115–122[PubMed]
    [Google Scholar]
  12. Casais R, Thiel V, Siddell SG, Cavanagh D, Britton P. Reverse genetics system for the avian coronavirus infectious bronchitis virus. J Virol 2001;75:12359–12369 [CrossRef][PubMed]
    [Google Scholar]
  13. Casais R, Dove B, Cavanagh D, Britton P. Recombinant avian infectious bronchitis virus expressing a heterologous spike gene demonstrates that the spike protein is a determinant of cell tropism. J Virol 2003;77:9084–9089 [CrossRef][PubMed]
    [Google Scholar]
  14. Bickerton E, Maier HJ, Stevenson-Leggett P, Armesto M, Britton P. The S2 subunit of infectious bronchitis virus beaudette is a determinant of cellular tropism. J Virol 2018;92: [CrossRef][PubMed]
    [Google Scholar]
  15. Frazatti-Gallina NM, Mourão-Fuches RM, Paoli RL, Silva ML, Miyaki C et al. Vero-cell rabies vaccine produced using serum-free medium. Vaccine 2004;23:511–517 [CrossRef][PubMed]
    [Google Scholar]
  16. Montagnon BJ, Fanget B, Nicolas AJ. The large-scale cultivation of VERO cells in micro-carrier culture for virus vaccine production. Preliminary results for killed poliovirus vaccine. Dev Biol Stand 1981;47:55–64[PubMed]
    [Google Scholar]
  17. Govorkova EA, Murti G, Meignier B, de Taisne C, Webster RG. African green monkey kidney (Vero) cells provide an alternative host cell system for influenza A and B viruses. J Virol 1996;70:5519–5524[PubMed]
    [Google Scholar]
  18. Kistner O, Barrett PN, Mundt W, Reiter M, Schober-Bendixen S et al. Development of a mammalian cell (Vero) derived candidate influenza virus vaccine. Vaccine 1998;16:960–968 [CrossRef][PubMed]
    [Google Scholar]
  19. Beaudette FR, Hudson CB. Cultivation of the virus of infectious bronchitis. J Am Vet Med Assoc 1937;90:51–60
    [Google Scholar]
  20. Cavanagh D, Davis PJ, Pappin DJ, Binns MM, Boursnell ME et al. Coronavirus IBV: partial amino terminal sequencing of spike polypeptide S2 identifies the sequence Arg-Arg-Phe-Arg-Arg at the cleavage site of the spike precursor propolypeptide of IBV strains Beaudette and M41. Virus Res 1986;4:133–143 [CrossRef][PubMed]
    [Google Scholar]
  21. Bijlenga G, Cook JK, Gelb J, de Wit JJ. Development and use of the H strain of avian infectious bronchitis virus from the Netherlands as a vaccine: a review. Avian Pathol 2004;33:550–557 [CrossRef][PubMed]
    [Google Scholar]
  22. Wang YD, Wang YL, Zhang Z, Fan G, Jiang Y et al. Isolation and identification of glandular stomach type IBV (QX IBV) in chickens. Chinese Journal of Animal Quarantine 1998;15:1–3
    [Google Scholar]
  23. Bande F, Arshad SS, Omar AR, Hair-Bejo M, Mahmuda A et al. Global distributions and strain diversity of avian infectious bronchitis virus: a review. Anim Health Res Rev 2017;18:70–83 [CrossRef][PubMed]
    [Google Scholar]
  24. Worthington KJ, Currie RJ, Jones RC. A reverse transcriptase-polymerase chain reaction survey of infectious bronchitis virus genotypes in Western Europe from 2002 to 2006. Avian Pathol 2008;37:247–257 [CrossRef][PubMed]
    [Google Scholar]
  25. Hennion R, Hill G. The preparation of chicken kidney cell cultures for virus propagation. In Maier HJ, Bickerton E, Britton P. (editors) Coronaviruses: Methods and Protocolsvol. 1282 Methods in Molecular Biology Springer; 2015; pp.57–62
    [Google Scholar]
  26. Penzes Z, Tibbles K, Shaw K, Britton P, Brown TD et al. Characterization of a replicating and packaged defective RNA of avian coronavirus infectious bronchitis virus. Virology 1994;203:286–293 [CrossRef][PubMed]
    [Google Scholar]
  27. Pénzes Z, Wroe C, Brown TD, Britton P, Cavanagh D. Replication and packaging of coronavirus infectious bronchitis virus defective RNAs lacking a long open reading frame. J Virol 1996;70:8660–8668[PubMed]
    [Google Scholar]
  28. Stirrups K, Shaw K, Evans S, Dalton K, Cavanagh D et al. Leader switching occurs during the rescue of defective RNAs by heterologous strains of the coronavirus infectious bronchitis virus. J Gen Virol 2000;81:791–801 [CrossRef][PubMed]
    [Google Scholar]
  29. Britton P, Evans S, Dove B, Davies M, Casais R et al. Generation of a recombinant avian coronavirus infectious bronchitis virus using transient dominant selection. J Virol Methods 2005;123:203–211 [CrossRef][PubMed]
    [Google Scholar]
  30. Boursnell ME, Brown TD, Foulds IJ, Green PF, Tomley FM et al. Completion of the sequence of the genome of the coronavirus avian infectious bronchitis virus. J Gen Virol 1987;68:57–77 [CrossRef][PubMed]
    [Google Scholar]
  31. Bonfield JK, Smith K, Staden R. A new DNA sequence assembly program. Nucleic Acids Res 1995;23:4992–4999 [CrossRef][PubMed]
    [Google Scholar]
  32. Armesto M, Bentley K, Bickerton E, Keep S, Britton P et al. Coronavirus reverse genetics. In Bridgen A. (editor) Reverse Genetics of RNA Viruses: Applications and Perspectives United Kingdom: Wiley-Blackwell Press; 2012; pp.27–63
    [Google Scholar]
  33. Valastro V, Holmes EC, Britton P, Fusaro A, Jackwood MW et al. S1 gene-based phylogeny of infectious bronchitis virus: an attempt to harmonize virus classification. Infect Genet Evol 2016;39:349–364 [CrossRef][PubMed]
    [Google Scholar]
  34. Promkuntod N, van Eijndhoven RE, de Vrieze G, Gröne A, Verheije MH. Mapping of the receptor-binding domain and amino acids critical for attachment in the spike protein of avian coronavirus infectious bronchitis virus. Virology 2014;448:26–32 [CrossRef][PubMed]
    [Google Scholar]
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