%0 Journal Article %A Liu, Fang %A Wang, Honglei %A Du, Li %A Wei, Zeyu %A Zhang, Qiong %A Feng, Wen-hai %T MicroRNA-30c targets the interferon–alpha/beta receptor beta chain to promote type 2 PRRSV infection %D 2018 %J Journal of General Virology, %V 99 %N 12 %P 1671-1680 %@ 1465-2099 %R https://doi.org/10.1099/jgv.0.001166 %K IFNAR2 %K PRRSV %K miR-30c %K IFN-I %I Microbiology Society, %X Porcine reproductive and respiratory syndrome (PRRS) is one of the most important diseases in pigs. MicroRNAs (miRNAs) have emerged as an important regulator of virus–host cell interactions and miR-30c has been found to facilitate PRRSV replication. Here, we found that the interferon-alpha/beta receptor beta chain (IFNAR2) was down-regulated, while miR-30c was up-regulated during HV (a highly pathogenic type 2 PRRSV strain) and CH-1a (a classic type 2 PRRSV strain) infection. Subsequently, using bioinformatics analysis, we predicted that the IFNAR2 was targeted by miR-30c. A luciferase assay verified that the 3′ UTR of IFNAR2 was targeted by miR-30c, as a mutation on either the target sequence or the miR-30c seed sequence reversed the luciferase activity. In addition, miR-30c and IFNAR2 mRNA were physically co-localized in RNA-induced silencing complex (RISC). Importantly, we showed that miR-30c also impaired the induction of IFN-stimulated genes (ISGs) by targeting IFNAR2. Our findings further reveal the mechanism of miR-30c promoting PRRSV replication. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001166