1887

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Volume 99, Issue 9

Prevalence of Porcine teschovirus genotypes in Hunan, China: identification of novel viral species and genotypes Free

Abstract

Porcine teschovirus (PTV) comprises at least 13 genotypes (PTV 1–13). Here, the genotypes of field strains prevalent among pig populations in Hunan Province, China, were identified. Multiple PTV genotypes, including all genotypes except PTV 7 and 8, were found co-circulating in the pig populations, reflecting a high genetic diversity. Moreover, we identified nine novel PTV genotypes, provisionally designated as PTV 14–22. PTV 21-HuN41 and PTV 21-HuN42 were successfully isolated, and their nearly complete genomes were sequenced. Homology comparison of the polyprotein genes of PTV 21-HuN41–42 to those of other known PTVs revealed low identities, ranging from 70.1 to 71.9 % (nucleotide identity) and 75.4 to 77.6 % (amino acid identity). Moreover, PTV 21-HuN41–42 were identified as a novel teschovirus species (tentatively Teschovirus B), based on the analyses of phylogenetics and evolutionary divergence. The findings of this study are expected to greatly enrich our knowledge of PTV genotypes.

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Keyword(s): novel genotype , phylogenetic analysis , Porcine teschovirus and VP1 gene
© 2018 The Authors
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2018-07-30
2024-04-19
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References

  1. Zell R, Delwart E, Gorbalenya AE, Hovi T, King AMQ et al. ICTV Virus Taxonomy Profile: Picornaviridae. J Gen Virol 2017; 98:2421–2422 [View Article][PubMed]
     [Google Scholar]
  2. Villanova F, Cui S, Ai X, Leal É. Analysis of full-length genomes of porcine teschovirus (PTV) and the effect of purifying selection on phylogenetic trees. Arch Virol 2016; 161:1199–1208 [View Article][PubMed]
     [Google Scholar]
  3. Yang T, Yu X, Luo B, Yan M, Li R et al. Epidemiology and molecular characterization of Porcine teschovirus in Hunan, China. Transbound Emerg Dis 2018; 65:480–490 [View Article][PubMed]
     [Google Scholar]
  4. Boros Á, Nemes C, Pankovics P, Kapusinszky B, Delwart E et al. Porcine teschovirus in wild boars in Hungary. Arch Virol 2012; 157:1573–1578 [View Article][PubMed]
     [Google Scholar]
  5. Knowles NJ. Porcine enteric picornaviruses. Diseases of swine 2006; 9:337–354
     [Google Scholar]
  6. Trefny L. Hromadna onemocnemi vepru na Tesinsku. Zverolek Obz 1930; 23:235–236
     [Google Scholar]
  7. Prodelalova J, Malenovska H, Valicek L. Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea. Veterinární Medicína 2009; 54:461–466
     [Google Scholar]
  8. Deng MY, Millien M, Jacques-Simon R, Flanagan JK, Bracht AJ et al. Diagnosis of Porcine teschovirus encephalomyelitis in the Republic of Haiti. J Vet Diagn Invest 2012; 24:671–678 [View Article][PubMed]
     [Google Scholar]
  9. Dunne HW, Gobble JL, Hokanson JF, Kradel DC, Bubash GR. Porcine reproductive failure associated with a newly identified "SMEDI" group of picorna viruses. Am J Vet Res 1965; 26:1284–1297[PubMed]
     [Google Scholar]
  10. Lin W, Cui S, Zell R. Phylogeny and evolution of porcine teschovirus 8 isolated from pigs in China with reproductive failure. Arch Virol 2012; 157:1387–1391 [View Article][PubMed]
     [Google Scholar]
  11. Zhang CF, Cui SJ, Hu S, Zhang Z, Guo Q et al. Isolation and characterization of the first Chinese strain of porcine Teschovirus-8. J Virol Methods 2010; 167:208–213 [View Article][PubMed]
     [Google Scholar]
  12. Meyer RC, Woods GT, Simon J. Pneumonitis in an enterovirus infection in swine. J Comp Pathol 1966; 76:397–405 [View Article][PubMed]
     [Google Scholar]
  13. Matias FF, Arruda B, Stevenson G, Schwartz K, Madson D et al. Development of polioencephalomyelitis in cesarean-derived colostrum-deprived pigs following experimental inoculation with either Teschovirus A serotype 2 or serotype 11. Viruses 2017; 9:
     [Google Scholar]
  14. Salles MW, Scholes SF, Dauber M, Strebelow G, Wojnarowicz C et al. Porcine teschovirus polioencephalomyelitis in western Canada. J Vet Diagn Invest 2011; 23:367–373 [View Article][PubMed]
     [Google Scholar]
  15. Buitrago D, Cano-Gómez C, Agüero M, Fernandez-Pacheco P, Gómez-Tejedor C et al. A survey of porcine picornaviruses and adenoviruses in fecal samples in Spain. J Vet Diagn Invest 2010; 22:763–766 [View Article][PubMed]
     [Google Scholar]
  16. Sozzi E, Barbieri I, Lavazza A, Lelli D, Moreno A et al. Molecular characterization and phylogenetic analysis of VP1 of porcine enteric picornaviruses isolates in Italy. Transbound Emerg Dis 2010; 57:434–442 [View Article][PubMed]
     [Google Scholar]
  17. Cano-Gómez C, Fernández-Pinero J, García-Casado MA, Zell R, Jiménez-Clavero MA. Characterization of PTV-12, a newly described porcine teschovirus serotype: in vivo infection and cross-protection studies. J Gen Virol 2017; 98:1636–1645 [View Article][PubMed]
     [Google Scholar]
  18. Zell R, Dauber M, Krumbholz A, Henke A, Birch-Hirschfeld E et al. Porcine teschoviruses comprise at least eleven distinct serotypes: molecular and evolutionary aspects. J Virol 2001; 75:1620–1631 [View Article][PubMed]
     [Google Scholar]
  19. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 2013; 30:2725–2729 [View Article][PubMed]
     [Google Scholar]
  20. Darriba D, Taboada GL, Doallo R, Posada D. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 2012; 9:772 [View Article][PubMed]
     [Google Scholar]
  21. Martin DP, Murrell B, Golden M, Khoosal A, Muhire B. RDP4: detection and analysis of recombination patterns in virus genomes. Virus Evol 2015; 1:v3 [View Article][PubMed]
     [Google Scholar]
  22. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS et al. Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 1999; 73:152–160[PubMed]
     [Google Scholar]
  23. Cano-Gómez C, Palero F, Buitrago MD, García-Casado MA, Fernández-Pinero J et al. Analyzing the genetic diversity of teschoviruses in Spanish pig populations using complete VP1 sequences. Infect Genet Evol 2011; 11:2144–2150 [View Article][PubMed]
     [Google Scholar]
  24. Chiu SC, Hu SC, Chang CC, Chang CY, Huang CC et al. The role of porcine teschovirus in causing diseases in endemically infected pigs. Vet Microbiol 2012; 161:88–95 [View Article][PubMed]
     [Google Scholar]
  25. Sun H, Gao H, Chen M, Lan D, Hua X et al. New serotypes of porcine teschovirus identified in Shanghai, China. Arch Virol 2015; 160:831–835 [View Article][PubMed]
     [Google Scholar]
  26. Cano-Gómez C, García-Casado MA, Soriguer R, Palero F, Jiménez-Clavero MA. Teschoviruses and sapeloviruses in faecal samples from wild boar in Spain. Vet Microbiol 2013; 165:115–122 [View Article][PubMed]
     [Google Scholar]
  27. Wang B, Tian ZJ, Gong DQ, Li DY, Wang Y et al. Isolation of serotype 2 porcine teschovirus in China: evidence of natural recombination. Vet Microbiol 2010; 146:138–143 [View Article][PubMed]
     [Google Scholar]
  28. Qiu Z, Wang Z, Zhang B, Zhang J, Cui S. The prevalence of porcine teschovirus in the pig population in northeast of China. J Virol Methods 2013; 193:209–214 [View Article][PubMed]
     [Google Scholar]
  29. Jiménez-Clavero MA, Fernández C, Ortiz JA, Pro J, Carbonell G et al. Teschoviruses as indicators of porcine fecal contamination of surface water. Appl Environ Microbiol 2003; 69:6311–6315 [View Article][PubMed]
     [Google Scholar]
  30. Donin DG, de Arruda Leme R, Alfieri AF, Alberton GC, Alfieri AA. First report of Porcine teschovirus (PTV), Porcine sapelovirus (PSV) and Enterovirus G (EV-G) in pig herds of Brazil. Trop Anim Health Prod 2014; 46:523–528 [View Article][PubMed]
     [Google Scholar]
  31. Prodělalová J. The survey of porcine teschoviruses, sapeloviruses and enteroviruses B infecting domestic pigs and wild boars in the Czech Republic between 2005 and 2011. Infect Genet Evol 2012; 12:1447–1451 [View Article][PubMed]
     [Google Scholar]
  32. Son KY, Kim DS, Matthijnssens J, Kwon HJ, Park JG et al. Molecular epidemiology of Korean porcine sapeloviruses. Arch Virol 2014; 159:1175–1180 [View Article][PubMed]
     [Google Scholar]
  33. Wang L, Ge C, Wang D, Li Y, Hu J. The survey of porcine teschoviruses, porcine circovirus and porcine transmissible gastroenteritis virus infecting piglets in clinical specimens in China. Trop Anim Health Prod 2013; 45:1087–1091 [View Article][PubMed]
     [Google Scholar]
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Prevalence of Porcine teschovirus genotypes in Hunan, China: identification of novel viral species and genotypes
J. Gen. Virol. 99, 1261 (2018); https://doi.org/10.1099/jgv.0.001129
/content/journal/jgv/10.1099/jgv.0.001129
/content/journal/jgv/10.1099/jgv.0.001129
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