1887

Abstract

A novel negative-sense RNA virus, Aedes aegypti anphevirus, was recently identified in wild Aedes aegypti mosquitoes. We show that this virus is also present in the Aag2 Aedes aegypti cell line and characterize its complete genome and evolutionary history. The Aedes aegypti anphevirus genome is estimated to be 12 916 nucleotides in length, contains four genes and has a genome structure similar to that of other anpheviruses. Phylogenetically, Aedes aegypti anphevirus falls within an unclassified group of insect-specific viruses in the order Mononegavirales that form a sister-group to the chuviruses. Notably, the Aag2 cell line used here was also experimentally infected with dengue virus and naturally contained a Phasi Charoen-like virus and cell-fusing agent virus. All four viruses were at relatively high abundance, with 0.5 % of sequence reads assigned to Aedes aegypti anphevirus. The Aag2 cell line is therefore permissive to efficient co-infection with dengue virus and multiple insect-specific viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001079
2018-05-09
2019-11-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/99/6/832.html?itemId=/content/journal/jgv/10.1099/jgv.0.001079&mimeType=html&fmt=ahah

References

  1. Shi M, Neville P, Nicholson J, Eden JS, Imrie A et al. High-resolution metatranscriptomics reveals the ecological dynamics of mosquito-associated RNA viruses in western Australia. J Virol 2017;91:e00680-17 [CrossRef][PubMed]
    [Google Scholar]
  2. Tree MO, McKellar DR, Kieft KJ, Watson AM, Ryman KD et al. Insect-specific flavivirus infection is restricted by innate immunity in the vertebrate host. Virology 2016;497:81–91 [CrossRef][PubMed]
    [Google Scholar]
  3. Li CX, Shi M, Tian JH, Lin XD, Kang YJ et al. Unprecedented genomic diversity of RNA viruses in arthropods reveals the ancestry of negative-sense RNA viruses. Elife 2015;4:e05378 [CrossRef][PubMed]
    [Google Scholar]
  4. Shi M, Lin XD, Vasilakis N, Tian JH, Li CX et al. Divergent viruses discovered in arthropods and vertebrates revise the evolutionary history of the Flaviviridae and related viruses. J Virol 2016;90:659–669 [CrossRef][PubMed]
    [Google Scholar]
  5. Lewis SH, Quarles KA, Yang Y, Tanguy M, Frézal L et al. Pan-arthropod analysis reveals somatic piRNAs as an ancestral defence against transposable elements. Nat Ecol Evol 2018;2:174–181 [CrossRef][PubMed]
    [Google Scholar]
  6. Zakrzewski M, Rašić G, Darbro J, Krause L, Poo YS et al. Mapping the virome in wild-caught Aedes aegypti from Cairns and Bangkok. Sci Rep 2018;8:4690 [CrossRef][PubMed]
    [Google Scholar]
  7. Walker T, Johnson PH, Moreira LA, Iturbe-Ormaetxe I, Frentiu FD et al. The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations. Nature 2011;476:450–453 [CrossRef][PubMed]
    [Google Scholar]
  8. Barletta AB, Silva MC, Sorgine MH. Validation of Aedes aegypti Aag-2 cells as a model for insect immune studies. Parasit Vectors 2012;5:148 [CrossRef][PubMed]
    [Google Scholar]
  9. Varjak M, Donald CL, Mottram TJ, Sreenu VB, Merits A et al. Characterization of the Zika virus induced small RNA response in Aedes aegypti cells. PLoS Negl Trop Dis 2017;11:e0006010 [CrossRef][PubMed]
    [Google Scholar]
  10. Sim S, Dimopoulos G. Dengue virus inhibits immune responses in Aedes aegypti cells. PLoS One 2010;5:e10678 [CrossRef][PubMed]
    [Google Scholar]
  11. Peleg J. Growth of arboviruses in primary tissue culture of Aedes aegypti embryos. Am J Trop Med Hyg 1968;17:219–223 [CrossRef][PubMed]
    [Google Scholar]
  12. Chandler JA, Thongsripong P, Green A, Kittayapong P, Wilcox BA et al. Metagenomic shotgun sequencing of a Bunyavirus in wild-caught Aedes aegypti from Thailand informs the evolutionary and genomic history of the Phleboviruses. Virology 2014;464-465:312–319 [CrossRef][PubMed]
    [Google Scholar]
  13. Zhang G, Etebari K, Asgari S. Wolbachia suppresses cell fusing agent virus in mosquito cells. J Gen Virol 2016;97:3427–3432 [CrossRef][PubMed]
    [Google Scholar]
  14. Maringer K, Yousuf A, Heesom KJ, Fan J, Lee D et al. Proteomics informed by transcriptomics for characterising active transposable elements and genome annotation in Aedes aegypti. BMC Genomics 2017;18:101 [CrossRef][PubMed]
    [Google Scholar]
  15. Yamao T, Eshita Y, Kihara Y, Satho T, Kuroda M et al. Novel virus discovery in field-collected mosquito larvae using an improved system for rapid determination of viral RNA sequences (RDV ver4.0). Arch Virol 2009;154:153–158 [CrossRef][PubMed]
    [Google Scholar]
  16. Grabherr MG, Haas BJ, Yassour M, Levin JZ, Thompson DA et al. Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol 2011;29:644–652 [CrossRef][PubMed]
    [Google Scholar]
  17. Buchfink B, Xie C, Huson DH. Fast and sensitive protein alignment using DIAMOND. Nat Methods 2015;12:59–60 [CrossRef][PubMed]
    [Google Scholar]
  18. Kearse M, Moir R, Wilson A, Stones-Havas S, Cheung M et al. Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 2012;28:1647–1649 [CrossRef][PubMed]
    [Google Scholar]
  19. Langmead B, Salzberg SL. Fast gapped-read alignment with Bowtie 2. Nat Methods 2012;9:357–359 [CrossRef][PubMed]
    [Google Scholar]
  20. Hulo C, de Castro E, Masson P, Bougueleret L, Bairoch A et al. ViralZone: a knowledge resource to understand virus diversity. Nucleic Acids Res 2011;39:D576–D582 [CrossRef][PubMed]
    [Google Scholar]
  21. Pfaller CK, Cattaneo R, Schnell MJ. Reverse genetics of mononegavirales: how they work, new vaccines, and new cancer therapeutics. Virology 2015;479-480:331–344 [CrossRef][PubMed]
    [Google Scholar]
  22. Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 2013;30:772–780 [CrossRef][PubMed]
    [Google Scholar]
  23. Capella-Gutiérrez S, Silla-Martínez JM, Gabaldón T. trimAl: a tool for automated alignment trimming in large-scale phylogenetic analyses. Bioinformatics 2009;25:1972–1973 [CrossRef][PubMed]
    [Google Scholar]
  24. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 2003;52:696–704 [CrossRef][PubMed]
    [Google Scholar]
  25. Fauver JR, Grubaugh ND, Krajacich BJ, Weger-Lucarelli J, Lakin SM et al. West African Anopheles gambiae mosquitoes harbor a taxonomically diverse virome including new insect-specific flaviviruses, mononegaviruses, and totiviruses. Virology 2016;498:288–299 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001079
Loading
/content/journal/jgv/10.1099/jgv.0.001079
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error