1887

Abstract

The viral landscape of the honey bee () has changed as a consequence of the global spread of the parasitic mite and accompanying virulent strains of the iflavirus deformed wing virus (DWV), which the mite vectors. The presence of DWV in honey bee populations is known to influence the occurrence of other viruses, suggesting that the current known virome of may be undercharacterized. Here we tested this hypothesis by examining the honey bee virome in Australia, which is uniquely free of parasitic mites or DWV. Using a high-throughput sequencing (HTS) approach, we examined the RNA virome from nine pools of across Australia. In addition to previously reported honey bee viruses, several other insect viruses were detected, including strains related to aphid lethal paralysis virus (ALPV) and virus (RhPV), which have recently been identified as infecting honey bees in the USA, as well as several other viruses recently found in spp. A further 42 putative novel insect virus genomes spanning the order were assembled, which significantly increases the known viral diversity in Among these novel genomes, we identified several that were similar (but different) to key viruses, such as DWV, that warrant further investigation. We propose that may be preferentially infected with viruses of the order and that a diverse population of these viruses may be representative of a -free landscape.

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2018-06-01
2024-03-29
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