%0 Journal Article %A Abbadi, Miriam %A Zamperin, Gianpiero %A Gastaldelli, Michele %A Pascoli, Francesco %A Rosani, Umberto %A Milani, Adelaide %A Schivo, Alessia %A Rossetti, Emanuele %A Turolla, Edoardo %A Gennari, Lorenzo %A Toffan, Anna %A Arcangeli, Giuseppe %A Venier, Paola %T Identification of a newly described OsHV-1 µvar from the North Adriatic Sea (Italy) %D 2018 %J Journal of General Virology, %V 99 %N 5 %P 693-703 %@ 1465-2099 %R https://doi.org/10.1099/jgv.0.001042 %K OsHV-1-PT %K Crassostrea gigas %K complete genome %K de novo assembly %K next generation sequencing %K Ostreid herpesvirus type 1 μVar %I Microbiology Society, %X The surveillance activities for abnormal bivalve mortality events in Italy include the diagnosis of ostreid herpesvirus type 1 (OsHV-1) in symptomatic oysters. OsHV-1-positive oysters (Crassostrea gigas) were used as a source for in vivo virus propagation and a virus-rich sample was selected to perform shotgun sequencing based on Illumina technology. Starting from this unpurified supernatant sample from gills and mantle, we generated 3.5 million reads (2×300 bp) and de novo assembled the whole genome of an Italian OsHV-1 microvariant (OsHV-1-PT). The OsHV-1-PT genome encodes 125 putative ORFs, 7 of which had not previously been predicted in other sequenced Malacoherpesviridae. Overall, OsHV-1-PT displays typical microvariant OsHV-1 genome features, while few polymorphisms (0.08 %) determine its uniqueness. As little is known about the genetic determinants of OsHV-1 virulence, comparing complete OsHV-1 genomes supports a better understanding of the virus pathogenicity and provides new insights into virus–host interactions. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.001042