1887

Abstract

The surveillance activities for abnormal bivalve mortality events in Italy include the diagnosis of ostreid herpesvirus type 1 (OsHV-1) in symptomatic oysters. OsHV-1-positive oysters (Crassostrea gigas) were used as a source for in vivo virus propagation and a virus-rich sample was selected to perform shotgun sequencing based on Illumina technology. Starting from this unpurified supernatant sample from gills and mantle, we generated 3.5 million reads (2×300 bp) and de novo assembled the whole genome of an Italian OsHV-1 microvariant (OsHV-1-PT). The OsHV-1-PT genome encodes 125 putative ORFs, 7 of which had not previously been predicted in other sequenced Malacoherpesviridae. Overall, OsHV-1-PT displays typical microvariant OsHV-1 genome features, while few polymorphisms (0.08 %) determine its uniqueness. As little is known about the genetic determinants of OsHV-1 virulence, comparing complete OsHV-1 genomes supports a better understanding of the virus pathogenicity and provides new insights into virus–host interactions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001042
2018-03-26
2019-09-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/99/5/693.html?itemId=/content/journal/jgv/10.1099/jgv.0.001042&mimeType=html&fmt=ahah

References

  1. Nicolas JL, Comps M, Cochennec N. Herpes-like virus infecting Pacific oyster larvae, Crassostrea gigas. Bull Eur Ass Fish Pathol 1992; 12: 11
    [Google Scholar]
  2. Renault T, Le Deuff RM, Cochennec N, Maffart P. Herpesviruses associated with mortalities among Pacific oyster, Crassostrea gigas, in France – comparative study. Rev Med Vet 1994; 145: 735– 742
    [Google Scholar]
  3. Burge CA, Griffin FJ, Friedman CS. Mortality and herpesvirus infections of the Pacific oyster Crassostrea gigas in Tomales Bay, California, USA. Dis Aquat Organ 2006; 72: 31– 43 [CrossRef] [PubMed]
    [Google Scholar]
  4. Renault T, Moreau P, Faury N, Pepin JF, Segarra A et al. Analysis of clinical Ostreid herpesvirus 1 (Malacoherpesviridae) specimens by sequencing amplified fragments from three virus genome areas. J Virol 2012; 86: 5942– 5947 [CrossRef] [PubMed]
    [Google Scholar]
  5. Paul-Pont I, Dhand NK, Whittington RJ. Spatial distribution of mortality in Pacific oysters Crassostrea gigas: reflection on mechanisms of OsHV-1 transmission. Dis Aquat Organ 2013; 105: 127– 138 [CrossRef] [PubMed]
    [Google Scholar]
  6. EFSA, European Food Safety Authority EFSA Panel on Animal Health and Welfare (AHAW): scientific opinion on oyster mortality. EFSA J 2015; 13: 4122– 4180 [Crossref]
    [Google Scholar]
  7. OIE, Office International des Epizooties Infection with Ostreid herpesvirus 1 microvariants. Manual of Diagnostic for Aquatic Animals Chap. 2.4.5 2015
    [Google Scholar]
  8. Bai C, Gao W, Wang C, Yu T, Zhang T et al. Identification and characterization of Ostreid herpesvirus 1 associated with massive mortalities of Scapharca broughtonii broodstocks in China. Dis Aquat Organ 2016; 118: 65– 75 [CrossRef] [PubMed]
    [Google Scholar]
  9. Davison AJ, Trus BL, Cheng N, Steven AC, Watson MS et al. A novel class of herpesvirus with bivalve hosts. J Gen Virol 2005; 86: 41– 53 [CrossRef] [PubMed]
    [Google Scholar]
  10. Segarra A, Pépin JF, Arzul I, Morga B, Faury N et al. Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008. Virus Res 2010; 153: 92– 99 [CrossRef] [PubMed]
    [Google Scholar]
  11. Jenkins C, Hick P, Gabor M, Spiers Z, Fell SA et al. Identification and characterisation of an Ostreid herpesvirus-1 microvariant (OsHV-1 µ-var) in Crassostrea gigas (Pacific oysters) in Australia. Dis Aquat Organ 2013; 105: 109– 126 [CrossRef] [PubMed]
    [Google Scholar]
  12. Burioli EAV, Prearo M, Riina MV, Bona MC, Fioravanti ML et al. Ostreid herpesvirus type 1 genomic diversity in wild populations of Pacific oyster Crassostrea gigas from Italian coasts. J Invertebr Pathol 2016; 137: 71– 83 [CrossRef] [PubMed]
    [Google Scholar]
  13. Burioli EAV, Prearo M, Houssin M. Complete genome sequence of Ostreid herpesvirus type 1 µVar isolated during mortality events in the Pacific oyster Crassostrea gigas in France and Ireland. Virology 2017; 509: 239– 251 [CrossRef] [PubMed]
    [Google Scholar]
  14. Renault T, Arzul I, Lipart C. Development and use of an internal standard for oyster herpesvirus 1 detection by PCR. J Virol Methods 2004; 121: 17– 23 [CrossRef] [PubMed]
    [Google Scholar]
  15. Webb SC, Fidler A, Renault T. Primers for PCR-based detection of ostreid herpes virus-1 (OsHV-1): application in a survey of New Zealand molluscs. Aquaculture 2007; 272: 126– 139 [CrossRef]
    [Google Scholar]
  16. Batista FM, Arzul I, Pepin JF, Ruano F, Friedman CS et al. Detection of Ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: a critical review. J Virol Methods 2007; 139: 1– 11 [CrossRef] [PubMed]
    [Google Scholar]
  17. Martenot C, Oden E, Travaillé E, Malas JP, Houssin M. Detection of different variants of Ostreid herpesvirus 1 in the Pacific oyster, Crassostrea gigas between 2008 and 2010. Virus Res 2011; 160: 25– 31 [CrossRef] [PubMed]
    [Google Scholar]
  18. Savin KW, Cocks BG, Wong F, Sawbridge T, Cogan N et al. A neurotropic herpesvirus infecting the gastropod, abalone, shares ancestry with oyster herpesvirus and a herpesvirus associated with the amphioxus genome. Virol J 2010; 7: 308 [CrossRef] [PubMed]
    [Google Scholar]
  19. Ren W, Chen H, Renault T, Cai Y, Bai C et al. Complete genome sequence of acute viral necrosis virus associated with massive mortality outbreaks in the Chinese scallop, Chlamys farreri. Virol J 2013; 10: 110 [CrossRef] [PubMed]
    [Google Scholar]
  20. Xia J, Bai C, Wang C, Song X, Huang J. Complete genome sequence of Ostreid herpesvirus-1 associated with mortalities of Scapharca broughtonii broodstocks. Virol J 2015; 12: 110 [CrossRef] [PubMed]
    [Google Scholar]
  21. FAO, Food and Agriculture Organisation of the United States Fisheries and Aquaculture Department, Dateset Global Aquaculture Production. 2017; On-Line Query
  22. Arzul I, Renault T, Lipart C, Davison AJ. Evidence for interspecies transmission of oyster herpesvirus in marine bivalves. J Gen Virol 2001; 82: 865– 870 [CrossRef] [PubMed]
    [Google Scholar]
  23. Schikorski D, Faury N, Pepin JF, Saulnier D, Tourbiez D et al. Experimental Ostreid herpesvirus 1 infection of the Pacific oyster Crassostrea gigas: kinetics of virus DNA detection by q-PCR in seawater and in oyster samples. Virus Res 2011; 155: 28– 34 [CrossRef] [PubMed]
    [Google Scholar]
  24. Schikorski D, Renault T, Saulnier D, Faury N, Moreau P et al. Experimental infection of Pacific oyster Crassostrea gigas spat by Ostreid herpesvirus 1: demonstration of oyster spat susceptibility. Vet Res 2011; 42: 27 [CrossRef] [PubMed]
    [Google Scholar]
  25. Paul-Pont I, Evans O, Dhand NK, Whittington RJ. Experimental infections of Pacific oyster Crassostrea gigas using the Australian Ostreid herpesvirus-1 (OsHV-1) µVar strain. Dis Aquat Organ 2015; 113: 137– 147 [CrossRef] [PubMed]
    [Google Scholar]
  26. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000; 25: 25– 29 [CrossRef] [PubMed]
    [Google Scholar]
  27. The Gene Ontology Consortium Expansion of the Gene Ontology knowledgebase and resources. Nucleic Acids Res 2017; 45: D331– D338 [CrossRef] [PubMed]
    [Google Scholar]
  28. Götz S, García-Gómez JM, Terol J, Williams TD, Nagaraj SH et al. High-throughput functional annotation and data mining with the Blast2GO suite. Nucleic Acids Res 2008; 36: 3420– 3435 [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001042
Loading
/content/journal/jgv/10.1099/jgv.0.001042
Loading

Data & Media loading...

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error