Construction and characterization of an infectious molecular clone of novel duck reovirus Free

Abstract

Novel duck reovirus (NDRV), the prototype strain of the species (ARV), is currently an infectious agent for ducks. Studies on NDRV replication and pathogenesis have been hampered by the lack of an available reverse-genetics system. In this study, a plasmid-based reverse-genetics system that is free of helper viruses has been developed. In this system, 10 full-length gene segments of wild-type NDRV TH11 strain are transfected into BSR-T7/5 cells that express bacteriophage T7 RNA polymerase. Production of infectious virus was shown by the inoculation of cell lysate derived from transfected cells into 10-day-old duck embryos. The growth kinetics and infectivity of the recombinant strains were identical to those of the wild-type strain. These viruses grew well and were genetically stable both and . Altogether, these results show the successful production of an infectious clone for NDRV. The infectious clone reported will be further used to elucidate the mechanisms of host tropism, viral replication and pathogenesis, as well as immunological changes induced by NDRV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.001036
2018-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/99/4/449.html?itemId=/content/journal/jgv/10.1099/jgv.0.001036&mimeType=html&fmt=ahah

References

  1. Benavente J, Martínez-Costas J. Avian reovirus: structure and biology. Virus Res 2007; 123:105–119 [View Article][PubMed]
    [Google Scholar]
  2. Zhang X, Tang J, Walker SB, O'Hara D, Nibert ML et al. Structure of avian orthoreovirus virion by electron cryomicroscopy and image reconstruction. Virology 2005; 343:25–35 [View Article][PubMed]
    [Google Scholar]
  3. Bányai K, Dandár E, Dorsey KM, Mató T, Palya V. The genomic constellation of a novel avian orthoreovirus strain associated with runting-stunting syndrome in broilers. Virus Genes 2011; 42:82–89 [View Article][PubMed]
    [Google Scholar]
  4. Heffels-Redmann U, Muller H, Kaleta EF. Structural and biological characteristics of reoviruses isolated from Muscovy ducks (Cairina moschata). Avian Pathol 1992; 21:481–491 [View Article][PubMed]
    [Google Scholar]
  5. Woźniakowski G, Samorek-Salamonowicz E, Gaweł A. Occurrence of reovirus infection in Muscovy ducks (Cairina moschata) in south western Poland. Pol J Vet Sci 2014; 17:299–305 [View Article][PubMed]
    [Google Scholar]
  6. Yun T, Yu B, Ni Z, Ye W, Chen L et al. Genomic characteristics of a novel reovirus from Muscovy duckling in China. Vet Microbiol 2014; 168:261–271 [View Article][PubMed]
    [Google Scholar]
  7. Chen ZY, Zhu YQ, Li CF, Liu GQ. Characterization of Novel Duck reovirus from Outbreaks in China. Emerg Infect Dis 2011; 18:1209–1211 [Crossref]
    [Google Scholar]
  8. Zhu YQ, Li CF, Bi ZL, Chen ZY, Meng CC et al. Molecular characterization of a novel reovirus isolated from Pekin ducklings in China. Arch Virol 2015; 160:365–369 [View Article][PubMed]
    [Google Scholar]
  9. Li N, Hong T, Wang Y, Wang Y, Yu K et al. The pathogenicity of novel duck reovirus in Cherry Valley ducks. Vet Microbiol 2016; 192:181–185 [View Article][PubMed]
    [Google Scholar]
  10. Liu Q, Zhang G, Huang Y, Ren G, Chen L et al. Isolation and characterization of a reovirus causing spleen necrosis in Pekin ducklings. Vet Microbiol 2011; 148:200–206 [View Article][PubMed]
    [Google Scholar]
  11. Zheng X, Wang D, Ning K, Liang T, Wang M et al. A duck reovirus variant with a unique deletion in the sigma C gene exhibiting high pathogenicity in Pekin ducklings. Virus Res 2016; 215:37–41 [View Article][PubMed]
    [Google Scholar]
  12. Johne R, Reetz J, Kaufer BB, Trojnar E. Generation of an avian-mammalian rotavirus reassortant by using a helper virus-dependent reverse genetics system. J Virol 2015; 90:1439–1443 [View Article][PubMed]
    [Google Scholar]
  13. Kobayashi T, Antar AA, Boehme KW, Danthi P, Eby EA et al. A plasmid-based reverse genetics system for animal double-stranded RNA viruses. Cell Host Microbe 2007; 1:147–157 [View Article][PubMed]
    [Google Scholar]
  14. Kawagishi T, Kanai Y, Tani H, Shimojima M, Saijo M et al. Reverse genetics for fusogenic bat-borne orthoreovirus associated with acute respiratory tract infections in humans: Role of outer capsid protein σC in viral replication and pathogenesis. PLoS Pathog 2016; 12:e1005455 [View Article][PubMed]
    [Google Scholar]
  15. Kaname Y, Celma CC, Kanai Y, Roy P. Recovery of African horse sickness virus from synthetic RNA. J Gen Virol 2013; 94:2259–2265 [View Article][PubMed]
    [Google Scholar]
  16. Boyce M, Celma CC, Roy P. Development of reverse genetics systems for bluetongue virus: recovery of infectious virus from synthetic RNA transcripts. J Virol 2008; 82:8339–8348 [View Article][PubMed]
    [Google Scholar]
  17. Pretorius JM, Huismans H, Theron J. Establishment of an entirely plasmid-based reverse genetics system for Bluetongue virus. Virology 2015; 486:71–77 [View Article][PubMed]
    [Google Scholar]
  18. Matsuo E, Saeki K, Roy P, Kawano J. Development of reverse genetics for Ibaraki virus to produce viable VP6-tagged IBAV. FEBS Open Bio 2015; 5:445–453 [View Article][PubMed]
    [Google Scholar]
  19. Yang T, Zhang J, Xu Q, Sun E, Li J et al. Development of a reverse genetics system for epizootic hemorrhagic disease virus and evaluation of novel strains containing duplicative gene rearrangements. J Gen Virol 2015; 96:2714–2720 [View Article][PubMed]
    [Google Scholar]
  20. Komoto S, Sasaki J, Taniguchi K. Reverse genetics system for introduction of site-specific mutations into the double-stranded RNA genome of infectious rotavirus. Proc Natl Acad Sci USA 2006; 103:4646–4651 [View Article][PubMed]
    [Google Scholar]
  21. Trask SD, Taraporewala ZF, Boehme KW, Dermody TS, Patton JT. Dual selection mechanisms drive efficient single-gene reverse genetics for rotavirus. Proc Natl Acad Sci USA 2010; 107:18652–18657 [View Article][PubMed]
    [Google Scholar]
  22. Kanai Y, Komoto S, Kawagishi T, Nouda R, Nagasawa N et al. Entirely plasmid-based reverse genetics system for rotaviruses. Proc Natl Acad Sci USA 2017; 114:2349–2354 [View Article][PubMed]
    [Google Scholar]
  23. Glass RI, Parashar U, Patel M, Gentsch J, Jiang B. Rotavirus vaccines: successes and challenges. J Infect 2014; 68:S9–S18 [View Article][PubMed]
    [Google Scholar]
  24. Navarro A, Trask SD, Patton JT. Generation of genetically stable recombinant rotaviruses containing novel genome rearrangements and heterologous sequences by reverse genetics. J Virol 2013; 87:6211–6220 [View Article][PubMed]
    [Google Scholar]
  25. Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS. An improved reverse genetics system for mammalian orthoreoviruses. Virology 2010; 398:194–200 [View Article][PubMed]
    [Google Scholar]
  26. Reed LJ, Muench H. A simple method of estimation of 50% end points. Am J Hyg 1938; 27:493–497
    [Google Scholar]
  27. Bi Z, Zhu Y, Chen ZY, Li CF, Meng CC et al. Prokaryotic expression and polyclonal antibody preparation of σC protein of novel duck reovirus. Acta Agriculturae Zhejiangensis 2014; 26:882–886 (in Chinese)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.001036
Loading
/content/journal/jgv/10.1099/jgv.0.001036
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most cited Most Cited RSS feed