1887

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Volume 99, Issue 3

Two novel thermally resistant endolysins encoded by pseudo T-even bacteriophages RB43 and RB49 Free

Abstract

Identification and cloning of genes as well as biochemical characterization of the gene products were carried out for two novel endolysins of pseudo T-even lytic bacteriophages RB43 and RB49, which represent different myovirus groups of the subfamily . Genes RB43ORF159c and RB49р102 were cloned in cells, and their products were purified to electrophoretic homogeneity with an up to 80 % yield of total activity. In respect to substrate specificity, both enzymes were found to be lytic -alanoyl--glutamate peptidases belonging to the M15 family. The pH optimum functioning of both endolysins was within the range 7.0–9.0, whereas the optimal values of ionic strength were different for the two proteins (25 mM vs 100 mM for the RB43 and RB49 endolysins respectively). Both peptidases were thermally resistant, with the RB43 endolysin being more stable (it restored 81 % of enzyme activity and 96 % of secondary structure after a 10 min heating at 90 °C) than its RB49 counterpart (27 and 77% respectively). The possible origin of genes of lytic -alanoyl--glutamate peptidases of myoviruses as a result of horizontal transfer in the variable parts of genomes between unrelated phages having a common host is discussed.

  • Received:
  • Accepted:
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Keyword(s): bacterial lysis , bacteriophage , endolysin , horizontal gene transfer , l-alanoyl-d-glutamate peptidase and pseudo T-even
© 2018 The Authors
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2018-03-01
2024-04-19
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Two novel thermally resistant endolysins encoded by pseudo T-even bacteriophages RB43 and RB49
J. Gen. Virol. 99, 402 (2018); https://doi.org/10.1099/jgv.0.001014
/content/journal/jgv/10.1099/jgv.0.001014
/content/journal/jgv/10.1099/jgv.0.001014
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