1887

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Volume 99, Issue 3

Specific transcriptional and post-transcriptional regulation of the major immediate early gene of GaHV-2 during the lytic, latent and reactivation phases Free

Abstract

Transcriptional and post-transcriptional mechanisms are involved in the switch between the lytic, latent and reactivation phases of the viral cycle in herpesviruses. During the productive phases, herpesvirus gene expression is characterized by a temporally regulated cascade of immediate early (IE), early (E) and late (L) genes. In alphaherpesviruses, the major product of the IE gene is a transcriptional regulator that initiates the cascade of gene expression that is essential for viral replication. In this study, we redefine the infected cell protein 4 () gene of the oncogenic Marek’s disease virus (MDV or gallid herpesvirus 2) as a 9438 nt gene ended with four alternative poly(A) signals and controlled by two alternative promoters containing essentially ubiquitous functional response elements (GC, TATA and CCAAT boxes). The distal promoter is associated with gene expression during the lytic and the latent phases, whereas the proximal promoter is associated with the expression of this gene during the reactivation phase. Both promoters are regulated by DNA methylation during the viral cycle and are hypermethylated during latency. Transcript analyses showed to consist of three exons and two introns, the alternative splicing of which is associated with five predicted nested ORFs. We show that the gene is highly and specifically regulated by transcriptional and post-transcriptional mechanisms during the three phases of the GaHV-2 viral cycle, with a clear difference in expression between the lytic phase and reactivation from latency in our model.

  • Received:
  • Accepted:
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Keyword(s): epigenetic , GaHV-2 , herpesvirus , ICP4 , splicing and transcriptional regulation
© 2018 The Authors
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2018-03-01
2024-04-20
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Specific transcriptional and post-transcriptional regulation of the major immediate early ICP4 gene of GaHV-2 during the lytic, latent and reactivation phases
J. Gen. Virol. 99, 355 (2018); https://doi.org/10.1099/jgv.0.001007
/content/journal/jgv/10.1099/jgv.0.001007
/content/journal/jgv/10.1099/jgv.0.001007
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