1887

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Volume 99, Issue 2

The use of novel epitope-tagged arenaviruses reveals that Rab5c-positive endosomal membranes are targeted by the LCMV matrix protein Free

Abstract

We report the development of recombinant New World (Junín; JUNV) and Old World (lymphocytic choriomeningitis virus; LCMV) mammarenaviruses that encode an HA-tagged matrix protein (Z). These viruses permit the robust affinity purification of Z from infected cells or virions, as well as the detection of Z by immunofluorescent microscopy. Importantly, the HA-tagged viruses grow with wild-type kinetics in a multi-cycle growth assay. Using these viruses, we report a novel description of JUNV Z localization in infected cells, as well as the first description of colocalization between LCMV Z and the GTPase Rab5c. This latter result, when combined with our previous findings that LCMV genome and glycoprotein also colocalize with Rab5c, suggest that LCMV may target Rab5c-positive membranes for preassembly of virus particles prior to budding. The recombinant viruses reported here will provide the field with new tools to better study Z protein functionality and identify key Z protein interactions with host machinery.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): arenavirus , HA epitope tag , Junín (JUNV) , lymphocytic choriomeningitis (LCMV) , Matrix protein (Z) and Rab5c
© 2018 The Authors
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/content/journal/jgv/10.1099/jgv.0.001004
2018-02-01
2023-09-23
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The use of novel epitope-tagged arenaviruses reveals that Rab5c-positive endosomal membranes are targeted by the LCMV matrix protein
J. Gen. Virol. 99, 187 (2018); https://doi.org/10.1099/jgv.0.001004
/content/journal/jgv/10.1099/jgv.0.001004
/content/journal/jgv/10.1099/jgv.0.001004
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