1887

Abstract

Species D is the largest of the seven species of human mastadenoviruses (HAdV), but few of its multiple types are associated with asevere disease, e.g. epidemic keratoconjunctivitis. Many other types are hardly ever associated with significant diseases in immunocompetent patients, but have been isolated from the diarrhoeal faeces of terminal AIDS patients suggesting their role as opportunistic pathogens. Three novel HAdV-D strains were isolated from the faeces of three immunocompromised adult patients (clinical diagnoses: lymphoma, myelodysplastic syndrome and AIDS CDC3B, respectively). These strains were not typeable by imputed serology of the hexon and fibre gene and therefore complete genomic sequences were generated by next-generation sequencing (NGS). All three strains were multiple recombinants and fulfilled the criteria for designation as types 73, 74 and 75 with the penton/hexon/fibre genotype codes P67H45F27, P70H74F51 and P75H26F29, respectively. A novel genomic backbone and also a novel hexon neutralization epitope sequence were discovered in type 74, and a novel penton sequence in type 75. At the complete genome level, types 73, 74 and 75 were closely related neither to each other nor to type 70, which was previously isolated in the same region. However, these four HAdV-D types were closely related to each other in single genes and gene regions, e.g. penton, E1 and E4 due to recombination events in their phylogeny. In conclusion, regional co-circulation of opportunistic HAdV-D types facilitated co- and super-infections, which are essential for homologous recombination, and thus resulted in the evolution of novel genotypes by lateral gene transfer.

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2017-11-02
2019-09-18
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References

  1. Ryan MA, Gray GC, Smith B, McKeehan JA, Hawksworth AW et al. Large epidemic of respiratory illness due to adenovirus types 7 and 3 in healthy young adults. Clin Infect Dis 2002;34:577–582 [CrossRef][PubMed]
    [Google Scholar]
  2. Jones MS, Harrach B, Ganac RD, Gozum MM, Dela Cruz WP et al. New adenovirus species found in a patient presenting with gastroenteritis. J Virol 2007;81:5978–5984 [CrossRef][PubMed]
    [Google Scholar]
  3. Walsh MP, Chintakuntlawar A, Robinson CM, Madisch I, Harrach B et al. Evidence of molecular evolution driven by recombination events influencing tropism in a novel human adenovirus that causes epidemic keratoconjunctivitis. PLoS One 2009;4:e5635 [CrossRef][PubMed]
    [Google Scholar]
  4. Lion T. Adenovirus infections in immunocompetent and immunocompromised patients. Clin Microbiol Rev 2014;27:441–462 [CrossRef][PubMed]
    [Google Scholar]
  5. Mynarek M, Ganzenmueller T, Mueller-Heine A, Mielke C, Gonnermann A et al. Patient, virus, and treatment-related risk factors in pediatric adenovirus infection after stem cell transplantation: results of a routine monitoring program. Biol Blood Marrow Transplant 2014;20:250–256 [CrossRef][PubMed]
    [Google Scholar]
  6. Ganzenmueller T, Buchholz S, Harste G, Dammann E, Trenschel R et al. High lethality of human adenovirus disease in adult allogeneic stem cell transplant recipients with high adenoviral blood load. J Clin Virol 2011;52:55–59 [CrossRef][PubMed]
    [Google Scholar]
  7. Hage E, Liebert GU, Bergs S, Ganzenmueller T, Heim A. Human mastadenovirus type 70: a novel, multiple recombinant species D mastadenovirus isolated from diarrhoeal faeces of a haematopoietic stem cell transplantation recipient. J Gen Virol 2015;96:2734–2742 [CrossRef][PubMed]
    [Google Scholar]
  8. Robinson CM, Seto D, Jones MS, Dyer DW, Chodosh J. Molecular evolution of human species D adenoviruses. Infect Genet Evol 2011;11:1208–1217 [CrossRef][PubMed]
    [Google Scholar]
  9. Robinson CM, Singh G, Lee JY, Dehghan S, Rajaiya J et al. Molecular evolution of human adenoviruses. Sci Rep 2013;3:1812 [CrossRef][PubMed]
    [Google Scholar]
  10. Singh G, Robinson CM, Dehghan S, Jones MS, Dyer DW et al. Homologous recombination in E3 genes of human adenovirus species D. J Virol 2013;87:12481–12488 [CrossRef][PubMed]
    [Google Scholar]
  11. Rosen L. A hemagglutination-inhibition technique for typing adenoviruses. Am J Hyg 1960;71:120–128[PubMed]
    [Google Scholar]
  12. Madisch I, Wölfel R, Harste G, Pommer H, Heim A. Molecular identification of adenovirus sequences: a rapid scheme for early typing of human adenoviruses in diagnostic samples of immunocompetent and immunodeficient patients. J Med Virol 2006;78:1210–1217 [CrossRef][PubMed]
    [Google Scholar]
  13. Wigand R. Pitfalls in the identification of adenoviruses. J Virol Methods 1987;16:161–169 [CrossRef][PubMed]
    [Google Scholar]
  14. Seto D, Chodosh J, Brister JR, Jones MS.Members of the Adenovirus Research Community Using the whole-genome sequence to characterize and name human adenoviruses. J Virol 2011;85:5701–5702 [CrossRef][PubMed]
    [Google Scholar]
  15. Khoo SH, Bailey AS, de Jong JC, Mandal BK. Adenovirus infections in human immunodeficiency virus-positive patients: clinical features and molecular epidemiology. J Infect Dis 1995;172:629–637 [CrossRef][PubMed]
    [Google Scholar]
  16. Ivanova OE, Yurashko OV, Eremeeva TP, Baikova OY, Morozova NS et al. Adenovirus isolation rates in acute flaccid paralysis patients. J Med Virol 2012;84:75–80 [CrossRef][PubMed]
    [Google Scholar]
  17. Kosulin K, Geiger E, Vécsei A, Huber WD, Rauch M et al. Persistence and reactivation of human adenoviruses in the gastrointestinal tract. Clin Microbiol Infect 2016;22:381.e1–381.e8 [CrossRef][PubMed]
    [Google Scholar]
  18. Lynch JP, Fishbein M, Echavarria M. Adenovirus. Semin Respir Crit Care Med 2011;32:494–511 [CrossRef][PubMed]
    [Google Scholar]
  19. Lukashev AN, Ivanova OE, Eremeeva TP, Iggo RD. Evidence of frequent recombination among human adenoviruses. J Gen Virol 2008;89:380–388 [CrossRef][PubMed]
    [Google Scholar]
  20. De Jong JC, Wermenbol AG, Verweij-Uijterwaal MW, Slaterus KW, Wertheim-van Dillen P et al. Adenoviruses from human immunodeficiency virus-infected individuals, including two strains that represent new candidate serotypes Ad50 and Ad51 of species B1 and D, respectively. J Clin Microbiol 1999;37:3940–3945[PubMed]
    [Google Scholar]
  21. Hierholzer JC, Wigand R, Anderson LJ, Adrian T, Gold JW. Adenoviruses from patients with AIDS: a plethora of serotypes and a description of five new serotypes of subgenus D (types 43–47). J Infect Dis 1988;158:804–813 [CrossRef][PubMed]
    [Google Scholar]
  22. Echavarría M. Adenoviruses in immunocompromised hosts. Clin Microbiol Rev 2008;21:704–715 [CrossRef][PubMed]
    [Google Scholar]
  23. Alissa Alkhalaf M, Al Qurashi YM, Guiver M, Cooper RJ. Genome sequences of three species d adenoviruses isolated from AIDS patients. Genome Announc 2014;2:e01267-13 [CrossRef][PubMed]
    [Google Scholar]
  24. Hage E, Huzly D, Ganzenmueller T, Beck R, Schulz TF et al. A human adenovirus species B subtype 21a associated with severe pneumonia. J Infect 2014;69:490–499 [CrossRef][PubMed]
    [Google Scholar]
  25. Cheng CY, Gilson T, Wimmer P, Schreiner S, Ketner G et al. Role of E1B55K in E4orf6/E1B55K E3 ligase complexes formed by different human adenovirus serotypes. J Virol 2013;87:6232–6245 [CrossRef][PubMed]
    [Google Scholar]
  26. Madisch I, Harste G, Pommer H, Heim A. Phylogenetic analysis of the main neutralization and hemagglutination determinants of all human adenovirus prototypes as a basis for molecular classification and taxonomy. J Virol 2005;79:15265–15276 [CrossRef][PubMed]
    [Google Scholar]
  27. Lole KS, Bollinger RC, Paranjape RS, Gadkari D, Kulkarni SS et al. Full-length human immunodeficiency virus type 1 genomes from subtype C-infected seroconverters in India, with evidence of intersubtype recombination. J Virol 1999;73:152–160[PubMed]
    [Google Scholar]
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