RT Journal Article SR Electronic(1) A1 Yang, Taotao A1 Yu, Xinglong A1 Yan, Meijun A1 Luo, Binyu A1 Li, Runcheng A1 Qu, Tailong A1 Luo, Zhang A1 Ge, Meng A1 Zhao, DunYR 2017 T1 Molecular characterization of Porcine sapelovirus in Hunan, China JF Journal of General Virology, VO 98 IS 11 SP 2738 OP 2747 DO https://doi.org/10.1099/jgv.0.000951 PB Microbiology Society, SN 1465-2099, AB Porcine sapeloviruses (PSVs) are widely distributed in pig populations; however, little information on their evolutionary history and the mechanisms driving their divergence is available. Therefore, in the present study, 241 fecal samples and 91 intestinal contents collected from pigs at 26 farms in Hunan, China, were tested for the presence of PSVs. The overall PSV positivity rate was 46.39 %, with a particularly high infection rate detected in nursery and fattening pigs. A total of 29 PSV strains (PSV-HuNs) were isolated, with these showing high genetic diversity based on phylogenetic and pairwise distance analyses of the capsid-protein gene sequences. Incongruence between phylognetic trees of the capsid-protein and 3CD regions indicated frequent recombination within the PSV-HuNs, and a putative recombinant hotspot near the 3′ end of the P1 region was identified. Our results suggested that recombination played an important role in driving PSV genetic diversity and evolution., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000951