1887

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Volume 98, Issue 11

Patchy DNA forms of the Zika virus RNA genome are generated following infection in mosquito cell cultures and in mosquitoes Free

Abstract

Zika virus (ZIKV) is a mosquito-borne flavivirus and has historically been reported to cause mild symptomatic diseases during human infections. More recently, the explosion of microcephaly among infants born to ZIKV-infected women has made ZIKV a global public health concern. While ZIKV causes acute human diseases, infections of vector mosquitoes are basically non-pathogenic, allowing persistent infections and conferring lifelong ability to transmit the virus. Recent studies have revealed that DNA forms of arboviral RNA genomes play a significant role in viral persistence in mosquitoes. We have initiated experiments to determine whether ZIKV generates viral DNA (vDNA) forms following infection in mosquitoes. Here we show that vDNAs are generated following ZIKV infection both in mosquito cell cultures and in its primary vector . vDNA formation is more extensive in RNA interference (RNAi)-deficient -derived C6/36 cells compared to RNAi-proficient mosquito cells. In addition, vDNAs are generated via multiple template-switching events.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Arbovirus , endogenous viral elements , Flavivirus , mosquito vectors , persistent infections and Zika virus
© Wadsworth Center, New York State Department of Health, 2017
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2017-11-01
2024-04-23
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Patchy DNA forms of the Zika virus RNA genome are generated following infection in mosquito cell cultures and in mosquitoes
J. Gen. Virol. 98, 2731 (2017); https://doi.org/10.1099/jgv.0.000945
/content/journal/jgv/10.1099/jgv.0.000945
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