@article{mbs:/content/journal/jgv/10.1099/jgv.0.000854, author = "Zhang, Chi and Yi, Lizhu and Feng, Shuangshuang and Liu, Xueqin and Su, Jianguo and Lin, Li and Tu, Jiagang", title = "MicroRNA miR-214 inhibits snakehead vesiculovirus replication by targeting the coding regions of viral N and P", journal= "Journal of General Virology", year = "2017", volume = "98", number = "7", pages = "1611-1619", doi = "https://doi.org/10.1099/jgv.0.000854", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000854", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "snakehead vesiculovirus", keywords = "replication", keywords = "interferon", keywords = "miR-214", keywords = "microRNA", abstract = "Snakeheadvesiculovirus (SHVV), a new member of the family Rhabdoviridae, has caused enormous economic losses in snakehead fish culture during the past years in China; however, little is known about the molecular mechanisms of its pathogenicity. MicroRNAs (miRNAs) are small non-coding RNAs that play important roles in virus infection. In this study, we identified that SHVV infection downregulated miR-214 in striped snakehead (SSN-1) cells in a time- and dose-dependent manner. Notably, transfecting SSN-1 cells with miR-214 mimic significantly inhibitedSHVV replication, whereas miR-214 inhibitor promoted it, suggesting that miR-214 acted as a negative regulator of SHVV replication. Our study further demonstrated that N and P of SHVV were the target genes of miR-214. Over-expression of P, but not N, inhibited IFN-α production in SHVV-infected cells, which could be restored by over-expression of miR-214. Taken together, these results suggest that miR-214 is downregulated during SHVV infection, and the downregulated miR-214 in turn increased N and P expression and decreased IFN-α production, thus facilitating SHVV replication. This study provides a better understanding of the molecular mechanisms on the pathogenesis of SHVV and a potential antiviral strategy against SHVV infection.", }