1887

Abstract

Chronic wasting disease (CWD) is an emergent prion disease affecting cervid species in North America, Canada, South Korea, and recently, Norway. Detection of CWD has been advanced by techniques that rely on amplification of low levels of prion amyloid to a detectable level. However, the increased sensitivity of amplification assays is often compromised by inhibitors and/or activators in complex biologic samples including body fluids, excreta, or the environment. Here, we adapt real-time quaking-induced conversion conditions to specifically detect CWD prions in fecal samples from both experimentally infected deer and naturally infected elk and estimate environmental contamination. The results have application to detection, surveillance and management of CWD, and potentially to other protein-misfolding diseases.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000844
2017-07-13
2019-09-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/98/7/1953.html?itemId=/content/journal/jgv/10.1099/jgv.0.000844&mimeType=html&fmt=ahah

References

  1. Sigurdson CJ. A prion disease of cervids: chronic wasting disease. Vet Res 2008;39:41 [CrossRef][PubMed]
    [Google Scholar]
  2. Williams ES. Chronic wasting disease. Vet Pathol 2005;42:530–549 [CrossRef][PubMed]
    [Google Scholar]
  3. Williams ES, Young S. Chronic wasting disease of captive mule deer: a spongiform encephalopathy. J Wildl Dis 1980;16:89–98 [CrossRef][PubMed]
    [Google Scholar]
  4. Miller MW, Williams ES, Hobbs NT, Wolfe LL. Environmental sources of prion transmission in mule deer. Emerg Infect Dis 2004;10:1003–1006 [CrossRef][PubMed]
    [Google Scholar]
  5. Mathiason CK, Powers JG, Dahmes SJ, Osborn DA, Miller KV et al. Infectious prions in the saliva and blood of deer with chronic wasting disease. Science 2006;314:133–136 [CrossRef][PubMed]
    [Google Scholar]
  6. Safar JG, Lessard P, Tamgüney G, Freyman Y, Deering C et al. Transmission and detection of prions in feces. J Infect Dis 2008;198:81–89 [CrossRef][PubMed]
    [Google Scholar]
  7. Haley NJ, Seelig DM, Zabel MD, Telling GC, Hoover EA. Detection of CWD prions in urine and saliva of deer by transgenic mouse bioassay. PLoS One 2009;4:e4848 [CrossRef][PubMed]
    [Google Scholar]
  8. Tamgüney G, Miller MW, Wolfe LL, Sirochman TM, Glidden DV et al. Asymptomatic deer excrete infectious prions in faeces. Nature 2009;461:529–532 [CrossRef][PubMed]
    [Google Scholar]
  9. Haley NJ, Mathiason CK, Carver S, Zabel M, Telling GC et al. Detection of chronic wasting disease prions in salivary, urinary, and intestinal tissues of deer: potential mechanisms of prion shedding and transmission. J Virol 2011;85:6309–6318 [CrossRef][PubMed]
    [Google Scholar]
  10. Henderson DM, Manca M, Haley NJ, Denkers ND, Nalls AV et al. Rapid antemortem detection of CWD prions in deer saliva. PLoS One 2013;8:e74377 [CrossRef][PubMed]
    [Google Scholar]
  11. Henderson DM, Denkers ND, Hoover CE, Garbino N, Mathiason CK et al. Longitudinal detection of prion shedding in Saliva and urine by chronic wasting disease-infected deer by real-time quaking-Induced conversion. J Virol 2015;89:9338–9347 [CrossRef][PubMed]
    [Google Scholar]
  12. Prusiner SB, Groth DF, Bolton DC, Kent SB, Hood LE. Purification and structural studies of a major scrapie prion protein. Cell 1984;38:127–134 [CrossRef][PubMed]
    [Google Scholar]
  13. Telling GC, Parchi P, Dearmond SJ, Cortelli P, Montagna P et al. Evidence for the conformation of the pathologic isoform of the prion protein enciphering and propagating prion diversity. Science 1996;274:2079–2082 [CrossRef][PubMed]
    [Google Scholar]
  14. Colby DW, Prusiner SB. Prions. Cold Spring Harb Perspect Biol 2011;3:a006833 [CrossRef][PubMed]
    [Google Scholar]
  15. Gabizon R, Telling G, Meiner Z, Halimi M, Kahana I et al. Insoluble wild-type and protease-resistant mutant prion protein in brains of patients with inherited prion disease. Nat Med 1996;2:59–64 [CrossRef][PubMed]
    [Google Scholar]
  16. Castilla J, Saá P, Hetz C, Soto C. In vitro generation of infectious scrapie prions. Cell 2005;121:195–206 [CrossRef][PubMed]
    [Google Scholar]
  17. Saborio GP, Permanne B, Soto C. Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding. Nature 2001;411:810–813 [CrossRef][PubMed]
    [Google Scholar]
  18. Atarashi R, Sano K, Satoh K, Nishida N. Real-time quaking-induced conversion: a highly sensitive assay for prion detection. Prion 2011;5:150–153 [CrossRef][PubMed]
    [Google Scholar]
  19. Wilham JM, Orrú CD, Bessen RA, Atarashi R, Sano K et al. Rapid end-point quantitation of prion seeding activity with sensitivity comparable to bioassays. PLoS Pathog 2010;6:e1001217 [CrossRef][PubMed]
    [Google Scholar]
  20. Colby DW, Zhang Q, Wang S, Groth D, Legname G et al. Prion detection by an amyloid seeding assay. Proc Natl Acad Sci USA 2007;104:20914–20919 [CrossRef][PubMed]
    [Google Scholar]
  21. Atarashi R, Satoh K, Sano K, Fuse T, Yamaguchi N et al. Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. Nat Med 2011;17:175–178 [CrossRef][PubMed]
    [Google Scholar]
  22. Mcguire LI, Peden AH, Orrú CD, Wilham JM, Appleford NE et al. Real time quaking-induced conversion analysis of cerebrospinal fluid in sporadic Creutzfeldt-Jakob disease. Ann Neurol 2012;72:278–285 [CrossRef][PubMed]
    [Google Scholar]
  23. Orrú CD, Wilham JM, Raymond LD, Kuhn F, Schroeder B et al. Prion disease blood test using immunoprecipitation and improved quaking-induced conversion. MBio 2011;2:e00078-11 [CrossRef][PubMed]
    [Google Scholar]
  24. Henderson DM, Davenport KA, Haley NJ, Denkers ND, Mathiason CK et al. Quantitative assessment of prion infectivity in tissues and body fluids by real-time quaking-induced conversion. J Gen Virol 2015;96:210–219 [CrossRef][PubMed]
    [Google Scholar]
  25. Zanusso G, Monaco S, Pocchiari M, Caughey B. Advanced tests for early and accurate diagnosis of Creutzfeldt-Jakob disease. Nat Rev Neurol 2016;12:325–333 [CrossRef][PubMed]
    [Google Scholar]
  26. Mcguire LI, Poleggi A, Poggiolini I, Suardi S, Grznarova K et al. Cerebrospinal fluid real-time quaking-induced conversion is a robust and reliable test for sporadic creutzfeldt-jakob disease: an international study. Ann Neurol 2016;80:160–165 [CrossRef][PubMed]
    [Google Scholar]
  27. Haley NJ, Siepker C, Walter WD, Thomsen BV, Greenlee JJ et al. Antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsy specimens from White-Tailed deer by real-time quaking-induced conversion. J Clin Microbiol 2016;54:1108–1116 [CrossRef][PubMed]
    [Google Scholar]
  28. Haley NJ, Siepker C, Hoon-Hanks LL, Mitchell G, Walter WD et al. Seeded amplification of chronic wasting disease prions in nasal brushings and recto-anal mucosa-associated lymphoid tissues from elk by real-time quaking-induced conversion. J Clin Microbiol 2016;54:1117–1126 [CrossRef][PubMed]
    [Google Scholar]
  29. Orrú CD, Groveman BR, Hughson AG, Zanusso G, Coulthart MB et al. Rapid and sensitive RT-QuIC detection of human Creutzfeldt-Jakob disease using cerebrospinal fluid. MBio 2015;6:e02451-14 [CrossRef][PubMed]
    [Google Scholar]
  30. Denkers ND, Henderson DM, Mathiason CK, Hoover EA. Enhanced prion detection in biological samples by magnetic particle extraction and real-time quaking-induced conversion. J Gen Virol 2016;97:2023–2029 [CrossRef][PubMed]
    [Google Scholar]
  31. Hoover CE, Davenport KA, Henderson DM, Pulscher LA, Mathiason CK et al. Detection and quantification of CWD Prions in fixed Paraffin embedded tissues by real-time auaking-induced conversion. Sci Rep 2016;6:25098 [CrossRef][PubMed]
    [Google Scholar]
  32. Rogers LL. Seasonal changes in defecation rates of free-ranging white-tailed deer. J Wildl Manage 1987;51:330–333 [CrossRef]
    [Google Scholar]
  33. Haley NJ, Mathiason CK, Zabel MD, Telling GC, Hoover EA. Detection of sub-clinical CWD infection in conventional test-negative deer long after oral exposure to urine and feces from CWD+ deer. PLoS One 2009;4:e7990 [CrossRef][PubMed]
    [Google Scholar]
  34. Pulford B, Spraker TR, Wyckoff AC, Meyerett C, Bender H et al. Detection of PrPCWD in feces from naturally exposed Rocky Mountain elk (Cervus elaphus nelsoni) using protein misfolding cyclic amplification. J Wildl Dis 2012;48:425–434 [CrossRef][PubMed]
    [Google Scholar]
  35. Saunders SE, Yuan Q, Bartz JC, Bartelt-Hunt S. Effects of solution chemistry and aging time on prion protein adsorption and replication of soil-bound prions. PLoS One 2011;6:e18752 [CrossRef][PubMed]
    [Google Scholar]
  36. Cheng YC, Hannaoui S, John TR, Dudas S, Czub S et al. Early and non-invasive detection of chronic wasting disease prions in elk feces by real-time quaking induced conversion. PLoS One 2016;11:e0166187 [CrossRef][PubMed]
    [Google Scholar]
  37. Renter DG, Gnad DP, Sargeant JM, Hygnstrom SE. Prevalence and serovars of Salmonella in the feces of free-ranging white-tailed deer (Odocoileus virginianus) in Nebraska. J Wildl Dis 2006;42:699–703 [CrossRef][PubMed]
    [Google Scholar]
  38. Reed PE, Mulangu S, Cameron KN, Ondzie AU, Joly D et al. A new approach for monitoring ebolavirus in wild great apes. PLoS Negl Trop Dis 2014;8:e3143 [CrossRef][PubMed]
    [Google Scholar]
  39. Denkers ND, Hayes-Klug J, Anderson KR, Seelig DM, Haley NJ et al. Aerosol transmission of chronic wasting disease in white-tailed deer. J Virol 2013;87:1890–1892 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000844
Loading
/content/journal/jgv/10.1099/jgv.0.000844
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error