1887

Abstract

G9P[8] rotavirus A (RVA) has been identified as the predominant genotype circulating in Yunnan, China. To elucidate the molecular characteristics of its genetic composition at the whole-genome level, the genomes of 12 strains isolated from paediatric patients with diarrhoea were fully sequenced and characterized. Eleven of the 12 strains were genotyped as G9-P[8]-I1-R1-C1-M1-A1-N1-T1-E1-H1, which is closely related to the Wa-like genotype 1 constellation. In contrast, one strain was genotyped as G9-P[8]-I1-R1-C1-M1-A1-N2-T1-E1-H1, with the NSP2 gene characterized as a DS-1 like genotype. Bayesian phylogenetic analysis indicated that G9 strains had emerged in 1932 with an estimated average evolutionary rate of 1.63×10 substitutions/site/year. Considering the high prevalence and fast evolutionary rate of G9P[8] rotaviruses, our results suggest that G9P[8] RVA should be strictly monitored in China.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/jgv.0.000807
2017-06-14
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/98/6/1163.html?itemId=/content/journal/jgv/10.1099/jgv.0.000807&mimeType=html&fmt=ahah

References

  1. Greenberg HB, Estes MK. Rotaviruses: from pathogenesis to vaccination. Gastroenterology 2009;136:1939–1951 [CrossRef][PubMed]
    [Google Scholar]
  2. Dennehy PH. Rotavirus vaccines—an update. Vaccine 2007;25:3137–3141 [CrossRef][PubMed]
    [Google Scholar]
  3. Li K, Lin XD, Huang KY, Zhang B, Shi M et al. Identification of novel and diverse rotaviruses in rodents and insectivores, and evidence of cross-species transmission into humans. Virology 2016;494:168–177 [CrossRef][PubMed]
    [Google Scholar]
  4. Wang CM, Chen SC, Chen KT. Current status of rotavirus vaccines. World J Pediatr 2015;11:300–308 [CrossRef][PubMed]
    [Google Scholar]
  5. Theamboonlers A, Maiklang O, Thongmee T, Chieochansin T, Vuthitanachot V et al. Complete genotype constellation of human rotavirus group A circulating in Thailand, 2008–2011. Infect Genet Evol 2014;21:295–302 [CrossRef][PubMed]
    [Google Scholar]
  6. Than VT, Baek IH, Kim W. Whole genomic analysis reveals the co-evolutionary phylodynamics of Korean G9P[8] human rotavirus strains. Arch Virol 2013;158:1795–1803 [CrossRef][PubMed]
    [Google Scholar]
  7. Nyaga MM, Jere KC, Peenze I, Mlera L, van Dijk AA et al. Sequence analysis of the whole genomes of five African human G9 rotavirus strains. Infect Genet Evol 2013;16:62–77 [CrossRef][PubMed]
    [Google Scholar]
  8. Wang X, Shi L, Qiao M, Wang Y, He M et al. [Phylogenetic analyses and characterization of the VP7 genes of serotype G9 rotaviruses circulating in Nanjing (China), 2012–2013]. Bing Du Xue Bao 2015;31:425–432[PubMed]
    [Google Scholar]
  9. Ghosh S, Kobayashi N. Whole-genomic analysis of rotavirus strains: current status and future prospects. Future Microbiol 2011;6:1049–1065 [CrossRef][PubMed]
    [Google Scholar]
  10. Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Bányai K et al. Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 2008;153:1621–1629 [CrossRef][PubMed]
    [Google Scholar]
  11. Dian Z, Fan M, Wang B, Feng Y, Ji H et al. The prevalence and genotype distribution of rotavirus A infection among children with acute gastroenteritis in Kunming, China. Arch Virol 2017;162:281–285 [CrossRef][PubMed]
    [Google Scholar]
  12. Wang YH, Pang BB, Ghosh S, Zhou X, Shintani T et al. Molecular epidemiology and genetic evolution of the whole genome of G3P[8] human rotavirus in Wuhan, China, from 2000 through 2013. PLoS One 2014;9:e88850 [CrossRef][PubMed]
    [Google Scholar]
  13. McDonald SM, Matthijnssens J, McAllen JK, Hine E, Overton L et al. Evolutionary dynamics of human rotaviruses: balancing reassortment with preferred genome constellations. PLoS Pathog 2009;5:e1000634 [CrossRef][PubMed]
    [Google Scholar]
  14. Yamamoto SP, Kaida A, Kubo H, Iritani N. Gastroenteritis outbreaks caused by a DS-1-like G1P[8] rotavirus strain, Japan, 2012-2013. Emerg Infect Dis 2014;20:1030–1033 [CrossRef][PubMed]
    [Google Scholar]
  15. Yamamoto SP, Kaida A, Ono A, Kubo H, Iritani N. Detection and characterization of a human G9P[4] rotavirus strain in Japan. J Med Virol 2015;87:1311–1318 [CrossRef][PubMed]
    [Google Scholar]
  16. Drummond AJ, Suchard MA, Xie D, Rambaut A. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol 2012;29:1969–1973 [CrossRef][PubMed]
    [Google Scholar]
  17. Doan YH, Nakagomi T, Agbemabiese CA, Nakagomi O. Changes in the distribution of lineage constellations of G2P[4] rotavirus A strains detected in Japan over 32 years (1980–2011). Infect Genet Evol 2015;34:423–433 [CrossRef][PubMed]
    [Google Scholar]
  18. Phan TG, Okitsu S, Maneekarn N, Ushijima H. Genetic heterogeneity, evolution and recombination in emerging G9 rotaviruses. Infect Genet Evol 2007;7:656–663 [CrossRef][PubMed]
    [Google Scholar]
  19. Teodoroff TA, Tsunemitsu H, Okamoto K, Katsuda K, Kohmoto M et al. Predominance of porcine rotavirus G9 in Japanese piglets with diarrhea: close relationship of their VP7 genes with those of recent human G9 strains. J Clin Microbiol 2005;43:1377–1384 [CrossRef][PubMed]
    [Google Scholar]
  20. Esona MD, Mijatovic-Rustempasic S, Foytich K, Roy S, Banyai K et al. Human G9P[8] rotavirus strains circulating in Cameroon, 1999–2000: genetic relationships with other G9 strains and detection of a new G9 subtype. Infect Genet Evol 2013;18:315–324 [CrossRef][PubMed]
    [Google Scholar]
  21. Zeller M, Patton JT, Heylen E, de Coster S, Ciarlet M et al. Genetic analyses reveal differences in the VP7 and VP4 antigenic epitopes between human rotaviruses circulating in Belgium and rotaviruses in Rotarix and RotaTeq. J Clin Microbiol 2012;50:966–976 [CrossRef][PubMed]
    [Google Scholar]
  22. Matthijnssens J, Heylen E, Zeller M, Rahman M, Lemey P et al. Phylodynamic analyses of rotavirus genotypes G9 and G12 underscore their potential for swift global spread. Mol Biol Evol 2010;27:2431–2436 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/jgv.0.000807
Loading
/content/journal/jgv/10.1099/jgv.0.000807
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error